A monoclonal antibody detects heterogeneity in vascular endothelium of tumours and normal tissues.

2.50
Hdl Handle:
http://hdl.handle.net/10541/99786
Title:
A monoclonal antibody detects heterogeneity in vascular endothelium of tumours and normal tissues.
Authors:
Wang, Ji Min; Kumar, Shant; Pye, David A; Van Agthoven, A J; Krupinski, Jerzy; Hunter, Robin D
Abstract:
A new murine monoclonal antibody (MAb), E-9, has been raised using tissue-cultured human umbilical vein endothelial cells. The antigen recognized by this MAb is a peptide of 170 kDa under non-reducing conditions and 96 kDa under reducing conditions. MAb E-9 showed marked heterogeneity in its distribution in various tissues. The antigen recognized by it was present in vascular endothelial cells of all tumours, foetal organs and in regenerating and inflamed tissues. It stained a few normal tissues. However, with the exception of tonsils, staining tended to be weak and limited to a few blood vessels, as revealed by double staining using pan-endothelial antibody (CD31) and antibody to von Willebrand factor, another marker of vascular endothelium. Surprisingly, blood vessels within the placental villi were completely negative. The function of the antigen recognized by MAb E-9 is not known, but its evaluation and use should increase our understanding of angiogenesis.
Affiliation:
Christie Hospital, Manchester, UK.
Citation:
A monoclonal antibody detects heterogeneity in vascular endothelium of tumours and normal tissues. 1993, 54 (3):363-70 Int. J. Cancer
Journal:
International Journal of Cancer
Issue Date:
28-May-1993
URI:
http://hdl.handle.net/10541/99786
DOI:
10.1002/ijc.2910540303
PubMed ID:
8509210
Type:
Article
Language:
en
ISSN:
0020-7136
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Ji Minen
dc.contributor.authorKumar, Shanten
dc.contributor.authorPye, David Aen
dc.contributor.authorVan Agthoven, A Jen
dc.contributor.authorKrupinski, Jerzyen
dc.contributor.authorHunter, Robin Den
dc.date.accessioned2010-05-25T13:59:42Z-
dc.date.available2010-05-25T13:59:42Z-
dc.date.issued1993-05-28-
dc.identifier.citationA monoclonal antibody detects heterogeneity in vascular endothelium of tumours and normal tissues. 1993, 54 (3):363-70 Int. J. Canceren
dc.identifier.issn0020-7136-
dc.identifier.pmid8509210-
dc.identifier.doi10.1002/ijc.2910540303-
dc.identifier.urihttp://hdl.handle.net/10541/99786-
dc.description.abstractA new murine monoclonal antibody (MAb), E-9, has been raised using tissue-cultured human umbilical vein endothelial cells. The antigen recognized by this MAb is a peptide of 170 kDa under non-reducing conditions and 96 kDa under reducing conditions. MAb E-9 showed marked heterogeneity in its distribution in various tissues. The antigen recognized by it was present in vascular endothelial cells of all tumours, foetal organs and in regenerating and inflamed tissues. It stained a few normal tissues. However, with the exception of tonsils, staining tended to be weak and limited to a few blood vessels, as revealed by double staining using pan-endothelial antibody (CD31) and antibody to von Willebrand factor, another marker of vascular endothelium. Surprisingly, blood vessels within the placental villi were completely negative. The function of the antigen recognized by MAb E-9 is not known, but its evaluation and use should increase our understanding of angiogenesis.en
dc.language.isoenen
dc.subjectCanceren
dc.subjectCancer Antigensen
dc.subjectExperimental Canceren
dc.subject.meshAdult-
dc.subject.meshAnimals-
dc.subject.meshAntibodies, Monoclonal-
dc.subject.meshAntigens-
dc.subject.meshAntigens, Neoplasm-
dc.subject.meshCattle-
dc.subject.meshCell Division-
dc.subject.meshCulture Techniques-
dc.subject.meshElectrophoresis, Polyacrylamide Gel-
dc.subject.meshEndothelium, Vascular-
dc.subject.meshFemale-
dc.subject.meshFlow Cytometry-
dc.subject.meshHumans-
dc.subject.meshImmunoglobulin G-
dc.subject.meshMice-
dc.subject.meshMice, Inbred BALB C-
dc.subject.meshMicroscopy, Fluorescence-
dc.subject.meshNeoplasms-
dc.subject.meshNeoplasms, Experimental-
dc.subject.meshPregnancy-
dc.subject.meshUmbilical Veins-
dc.titleA monoclonal antibody detects heterogeneity in vascular endothelium of tumours and normal tissues.en
dc.typeArticleen
dc.contributor.departmentChristie Hospital, Manchester, UK.en
dc.identifier.journalInternational Journal of Canceren

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