Irradiation induces up-regulation of E9 protein (CD105) in human vascular endothelial cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/99442
Title:
Irradiation induces up-regulation of E9 protein (CD105) in human vascular endothelial cells.
Authors:
Wang, Ji Min; Kumar, Shant; Van Agthoven, A; Kumar, Patricia; Pye, David A; Hunter, Robin D
Abstract:
The use of MAb E-9 raised against tissue-cultured endothelial cells (EC) has shown marked heterogeneity in vascular EC lining the blood vessels of normal and tumour tissues. MAb E-9 is human EC-specific and the protein recognized by it is a homodimer with a molecular mass of 97 kDa. The E-9 protein is resistant to treatment by 3 mM sodium periodate, but is sensitive to 10% trichloroacetic acid and 70% ethanol. E-9 protein has been assigned to a new cluster, CD105, and mapped to human chromosome 9q3. It has approximately 70% homology with type-III cell-surface receptor for transforming growth factor beta (TGF-beta). Recently CD105 has been reported to be the gene in patients with hereditary haemorrhagic telangiectasia. We have examined the effects of radiation on its expression in normal human umbilical-vein endothelial cells (HUVEC) and brain-tumour-derived endothelial cells (BTEC). Irradiation induced dose- and time-dependent up-regulation in the expression of the E-9 protein on the plasma membranes of EC, and also resulted in greater increase in the expression of the E-9 protein in semi-confluent (proliferating) as compared with confluent (non-proliferating) EC. It may well be that, following radiotherapy in cancer patients, E-9 protein is also up-regulated. The presence of increased amounts of E-9 protein in EC makes it an attractive target in the control of angiogenesis, especially after radiotherapy in cancer patients. The time scale involved in the up-regulation of E-9 protein following irradiation has led us to suggest that it may be a secondary event, the primary being the production and release of mitogenic factors (such as basic fibroblast growth factor) from irradiated EC.
Affiliation:
Christie Hospital, Manchester, UK.
Citation:
Irradiation induces up-regulation of E9 protein (CD105) in human vascular endothelial cells. 1995, 62 (6):791-6 Int. J. Cancer
Journal:
International Journal of Cancer
Issue Date:
15-Sep-1995
URI:
http://hdl.handle.net/10541/99442
DOI:
10.1002/ijc.2910620624
PubMed ID:
7558432
Type:
Article
Language:
en
ISSN:
0020-7136
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Ji Minen
dc.contributor.authorKumar, Shanten
dc.contributor.authorVan Agthoven, Aen
dc.contributor.authorKumar, Patriciaen
dc.contributor.authorPye, David Aen
dc.contributor.authorHunter, Robin Den
dc.date.accessioned2010-05-20T15:18:26Z-
dc.date.available2010-05-20T15:18:26Z-
dc.date.issued1995-09-15-
dc.identifier.citationIrradiation induces up-regulation of E9 protein (CD105) in human vascular endothelial cells. 1995, 62 (6):791-6 Int. J. Canceren
dc.identifier.issn0020-7136-
dc.identifier.pmid7558432-
dc.identifier.doi10.1002/ijc.2910620624-
dc.identifier.urihttp://hdl.handle.net/10541/99442-
dc.description.abstractThe use of MAb E-9 raised against tissue-cultured endothelial cells (EC) has shown marked heterogeneity in vascular EC lining the blood vessels of normal and tumour tissues. MAb E-9 is human EC-specific and the protein recognized by it is a homodimer with a molecular mass of 97 kDa. The E-9 protein is resistant to treatment by 3 mM sodium periodate, but is sensitive to 10% trichloroacetic acid and 70% ethanol. E-9 protein has been assigned to a new cluster, CD105, and mapped to human chromosome 9q3. It has approximately 70% homology with type-III cell-surface receptor for transforming growth factor beta (TGF-beta). Recently CD105 has been reported to be the gene in patients with hereditary haemorrhagic telangiectasia. We have examined the effects of radiation on its expression in normal human umbilical-vein endothelial cells (HUVEC) and brain-tumour-derived endothelial cells (BTEC). Irradiation induced dose- and time-dependent up-regulation in the expression of the E-9 protein on the plasma membranes of EC, and also resulted in greater increase in the expression of the E-9 protein in semi-confluent (proliferating) as compared with confluent (non-proliferating) EC. It may well be that, following radiotherapy in cancer patients, E-9 protein is also up-regulated. The presence of increased amounts of E-9 protein in EC makes it an attractive target in the control of angiogenesis, especially after radiotherapy in cancer patients. The time scale involved in the up-regulation of E-9 protein following irradiation has led us to suggest that it may be a secondary event, the primary being the production and release of mitogenic factors (such as basic fibroblast growth factor) from irradiated EC.en
dc.language.isoenen
dc.subject.meshAntibodies, Monoclonal-
dc.subject.meshAntigens, CD-
dc.subject.meshCell Adhesion-
dc.subject.meshCell Count-
dc.subject.meshCell Division-
dc.subject.meshCell Membrane-
dc.subject.meshCell Size-
dc.subject.meshCells, Cultured-
dc.subject.meshElectrophoresis-
dc.subject.meshEndothelium, Vascular-
dc.subject.meshFlow Cytometry-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshNeovascularization, Pathologic-
dc.subject.meshPrecipitin Tests-
dc.subject.meshReceptors, Cell Surface-
dc.subject.meshUp-Regulation-
dc.subject.meshVascular Cell Adhesion Molecule-1-
dc.titleIrradiation induces up-regulation of E9 protein (CD105) in human vascular endothelial cells.en
dc.typeArticleen
dc.contributor.departmentChristie Hospital, Manchester, UK.en
dc.identifier.journalInternational Journal of Canceren

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