The proliferation of normal human breast tissue implanted into athymic nude mice is stimulated by estrogen but not progesterone.

2.50
Hdl Handle:
http://hdl.handle.net/10541/99428
Title:
The proliferation of normal human breast tissue implanted into athymic nude mice is stimulated by estrogen but not progesterone.
Authors:
Laidlaw, I J; Clarke, Robert B; Howell, Anthony ( 0000-0002-3879-5991 ) ; Owen, A W; Potten, Christopher S; Anderson, Elizabeth
Abstract:
In order to resolve the question of which ovarian steroid stimulates normal human mammary epithelial cell proliferation, we have implanted pieces of normal human breast tissue subcutaneously into athymic nude mice. These mice were then treated with slow-release pellets containing estradiol (E2) or progesterone (P) such that serum levels of E2 and P were increased to those seen in normal women. The proliferative activity of the tissue implants was assessed by uptake of tritiated thymidine and steroid receptor expression was measured immunocytochemically. Insertion of a 2 mg E2 pellet 14 days after tissue implantation increased the thymidine labeling index (TLI) from a median of 0.4% (n = 34) to a median of 2.1% after 7 days (n = 43; P < 0.001 by Mann Whitney U test). In contrast, treatment with a P pellet (4 mg) had no effect upon the TLI whereas P (4 mg) in combination with E2 (2 mg) had no effect over and above that of E2 alone. There was a significant correlation between the increase in TLI and either the E2 content of the pellets (P < 0.001 by linear regression) or the serum E2 levels achieved (P < 0.001). Expression of the P receptor was increased 15- to 20-fold by E2 treatment. We conclude that E2 is sufficient to stimulate human breast epithelial cell proliferation at physiologically relevant concentrations and that P does not affect proliferation either alone or after E2 priming.
Affiliation:
Department of Surgery, University Hospital of South Manchester, United Kingdom.
Citation:
The proliferation of normal human breast tissue implanted into athymic nude mice is stimulated by estrogen but not progesterone. 1995, 136 (1):164-71 Endocrinology
Journal:
Endocrinology
Issue Date:
Jan-1995
URI:
http://hdl.handle.net/10541/99428
PubMed ID:
7828527
Type:
Article
Language:
en
ISSN:
0013-7227; 10.1210/en.136.1.164
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLaidlaw, I Jen
dc.contributor.authorClarke, Robert Ben
dc.contributor.authorHowell, Anthonyen
dc.contributor.authorOwen, A Wen
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorAnderson, Elizabethen
dc.date.accessioned2010-05-20T15:15:51Z-
dc.date.available2010-05-20T15:15:51Z-
dc.date.issued1995-01-
dc.identifier.citationThe proliferation of normal human breast tissue implanted into athymic nude mice is stimulated by estrogen but not progesterone. 1995, 136 (1):164-71 Endocrinologyen
dc.identifier.issn0013-7227-
dc.identifier.issn10.1210/en.136.1.164-
dc.identifier.pmid7828527-
dc.identifier.urihttp://hdl.handle.net/10541/99428-
dc.description.abstractIn order to resolve the question of which ovarian steroid stimulates normal human mammary epithelial cell proliferation, we have implanted pieces of normal human breast tissue subcutaneously into athymic nude mice. These mice were then treated with slow-release pellets containing estradiol (E2) or progesterone (P) such that serum levels of E2 and P were increased to those seen in normal women. The proliferative activity of the tissue implants was assessed by uptake of tritiated thymidine and steroid receptor expression was measured immunocytochemically. Insertion of a 2 mg E2 pellet 14 days after tissue implantation increased the thymidine labeling index (TLI) from a median of 0.4% (n = 34) to a median of 2.1% after 7 days (n = 43; P < 0.001 by Mann Whitney U test). In contrast, treatment with a P pellet (4 mg) had no effect upon the TLI whereas P (4 mg) in combination with E2 (2 mg) had no effect over and above that of E2 alone. There was a significant correlation between the increase in TLI and either the E2 content of the pellets (P < 0.001 by linear regression) or the serum E2 levels achieved (P < 0.001). Expression of the P receptor was increased 15- to 20-fold by E2 treatment. We conclude that E2 is sufficient to stimulate human breast epithelial cell proliferation at physiologically relevant concentrations and that P does not affect proliferation either alone or after E2 priming.en
dc.language.isoenen
dc.subjectOestrogen Receptorsen
dc.subject.meshAdult-
dc.subject.meshAnimals-
dc.subject.meshBreast-
dc.subject.meshCell Division-
dc.subject.meshEstradiol-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshMice-
dc.subject.meshMice, Nude-
dc.subject.meshProgesterone-
dc.subject.meshReceptors, Estrogen-
dc.subject.meshReceptors, Progesterone-
dc.subject.meshTissue Transplantation-
dc.subject.meshTransplantation, Heterologous-
dc.subject.meshTransplantation, Heterotopic-
dc.titleThe proliferation of normal human breast tissue implanted into athymic nude mice is stimulated by estrogen but not progesterone.en
dc.typeArticleen
dc.contributor.departmentDepartment of Surgery, University Hospital of South Manchester, United Kingdom.en
dc.identifier.journalEndocrinologyen
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