2.50
Hdl Handle:
http://hdl.handle.net/10541/99426
Title:
Alternate splicing produces a novel cyclin D1 transcript.
Authors:
Betticher, Daniel C; Thatcher, Nick; Altermatt, H J; Hoban, P; Ryder, W David J; Heighway, Jim
Abstract:
Using Northern blotting and PCR analysis of cDNA derived from a range of cell lines and tissues, alternate splicing of the cyclin D1 gene (CCND1) mRNA has been demonstrated. The variant transcript shows no splicing at the downstream exon 4 boundary, encoding a protein with an altered carboxy-terminal domain. Investigation of mRNA extracted from mononuclear cells, lung tumour and normal tissue suggests that both transcripts are invariably expressed. However, splicing to produce the two forms of mRNA is modulated, in the heterozygote, by a frequent A/G polymorphism located within the splice donor region of exon 4. Preliminary analysis of patients with resectable non-small cell lung cancer suggests that genotype is associated with shortened event free survival and greater risk of local relapse.
Affiliation:
CRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
Citation:
Alternate splicing produces a novel cyclin D1 transcript. 1995, 11 (5):1005-11 Oncogene
Journal:
Oncogene
Issue Date:
7-Sep-1995
URI:
http://hdl.handle.net/10541/99426
PubMed ID:
7675441
Type:
Article
Language:
en
ISSN:
0950-9232
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBetticher, Daniel Cen
dc.contributor.authorThatcher, Nicken
dc.contributor.authorAltermatt, H Jen
dc.contributor.authorHoban, Pen
dc.contributor.authorRyder, W David Jen
dc.contributor.authorHeighway, Jimen
dc.date.accessioned2010-05-20T15:11:40Z-
dc.date.available2010-05-20T15:11:40Z-
dc.date.issued1995-09-07-
dc.identifier.citationAlternate splicing produces a novel cyclin D1 transcript. 1995, 11 (5):1005-11 Oncogeneen
dc.identifier.issn0950-9232-
dc.identifier.pmid7675441-
dc.identifier.urihttp://hdl.handle.net/10541/99426-
dc.description.abstractUsing Northern blotting and PCR analysis of cDNA derived from a range of cell lines and tissues, alternate splicing of the cyclin D1 gene (CCND1) mRNA has been demonstrated. The variant transcript shows no splicing at the downstream exon 4 boundary, encoding a protein with an altered carboxy-terminal domain. Investigation of mRNA extracted from mononuclear cells, lung tumour and normal tissue suggests that both transcripts are invariably expressed. However, splicing to produce the two forms of mRNA is modulated, in the heterozygote, by a frequent A/G polymorphism located within the splice donor region of exon 4. Preliminary analysis of patients with resectable non-small cell lung cancer suggests that genotype is associated with shortened event free survival and greater risk of local relapse.en
dc.language.isoenen
dc.subjectLung Canceren
dc.subject.meshAged-
dc.subject.meshAlternative Splicing-
dc.subject.meshAmino Acid Sequence-
dc.subject.meshBase Sequence-
dc.subject.meshBlotting, Northern-
dc.subject.meshCarcinoma, Non-Small-Cell Lung-
dc.subject.meshCyclin D1-
dc.subject.meshCyclins-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshLung Neoplasms-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshOncogene Proteins-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshPolymorphism, Genetic-
dc.subject.meshRNA, Messenger-
dc.titleAlternate splicing produces a novel cyclin D1 transcript.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.en
dc.identifier.journalOncogeneen

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