2.50
Hdl Handle:
http://hdl.handle.net/10541/99021
Title:
Localisation and cellular origin of hyaluronectin.
Authors:
Ponting, Julie M; Kumar, Shant
Abstract:
Hyaluronectin is an extracellular matrix glycoprotein which specifically binds to hyaluronan. Isoforms of hyaluronectin are present in nervous and mesenchymal tissues but, while the nervous tissue isoform has been characterised in some detail, less is known about the mesenchymal isoform. Although its tissue localisation suggests a role in tumour development, neither its cellular origin nor its exact function are known. In this study we demonstrate hyaluronectin synthesis in fibroblasts and smooth muscle cells in vitro. The pattern of immunolocalisation of hyaluronectin in fibroblasts depended on the cell type, length of time spent by the cells in culture and cell density. Immunoreactivity in sparsely plated migratory cells was seen mainly in a patchy distribution at the attached cell surface and in the migration tracks left by the cells on the subtratum. In stationary cells a more uniform distribution associated with the attached cell surface was observed, while in confluent cultures hyaluronectin immunoreactivity was mainly seen as a network of fibrillar material above the cell. The pattern of staining was distinct from that of other hyaluronan-binding proteins. Immunoprecipitation, using antihyaluronectin antibodies, of the substratum-attached material deposited by human fetal fibroblasts revealed a family of proteins ranging from 22 to 90 kDa, the major protein being of approximately 60 kDa. These results lead us to propose that hyaluronectin plays an important role in cell migration, probably by regulation of hyaluronan distribution and binding.
Affiliation:
Department of Clinical Research, Christie Hospital, Manchester, UK.
Citation:
Localisation and cellular origin of hyaluronectin. 1995, 187 ( Pt 2):331-46 J. Anat.
Journal:
Journal of Anatomy
Issue Date:
Oct-1995
URI:
http://hdl.handle.net/10541/99021
PubMed ID:
7591996
Type:
Article
Language:
en
ISSN:
0021-8782
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorPonting, Julie Men
dc.contributor.authorKumar, Shanten
dc.date.accessioned2010-05-17T16:00:33Z-
dc.date.available2010-05-17T16:00:33Z-
dc.date.issued1995-10-
dc.identifier.citationLocalisation and cellular origin of hyaluronectin. 1995, 187 ( Pt 2):331-46 J. Anat.en
dc.identifier.issn0021-8782-
dc.identifier.pmid7591996-
dc.identifier.urihttp://hdl.handle.net/10541/99021-
dc.description.abstractHyaluronectin is an extracellular matrix glycoprotein which specifically binds to hyaluronan. Isoforms of hyaluronectin are present in nervous and mesenchymal tissues but, while the nervous tissue isoform has been characterised in some detail, less is known about the mesenchymal isoform. Although its tissue localisation suggests a role in tumour development, neither its cellular origin nor its exact function are known. In this study we demonstrate hyaluronectin synthesis in fibroblasts and smooth muscle cells in vitro. The pattern of immunolocalisation of hyaluronectin in fibroblasts depended on the cell type, length of time spent by the cells in culture and cell density. Immunoreactivity in sparsely plated migratory cells was seen mainly in a patchy distribution at the attached cell surface and in the migration tracks left by the cells on the subtratum. In stationary cells a more uniform distribution associated with the attached cell surface was observed, while in confluent cultures hyaluronectin immunoreactivity was mainly seen as a network of fibrillar material above the cell. The pattern of staining was distinct from that of other hyaluronan-binding proteins. Immunoprecipitation, using antihyaluronectin antibodies, of the substratum-attached material deposited by human fetal fibroblasts revealed a family of proteins ranging from 22 to 90 kDa, the major protein being of approximately 60 kDa. These results lead us to propose that hyaluronectin plays an important role in cell migration, probably by regulation of hyaluronan distribution and binding.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAdult-
dc.subject.meshAnimals-
dc.subject.meshAntigens, CD44-
dc.subject.meshCattle-
dc.subject.meshCells, Cultured-
dc.subject.meshChick Embryo-
dc.subject.meshExtracellular Matrix Proteins-
dc.subject.meshFemale-
dc.subject.meshFibroblasts-
dc.subject.meshFibronectins-
dc.subject.meshFluorescent Antibody Technique-
dc.subject.meshHumans-
dc.subject.meshMesoderm-
dc.subject.meshMuscle, Smooth-
dc.subject.meshSkin-
dc.subject.meshTalin-
dc.subject.meshTumor Cells, Cultured-
dc.titleLocalisation and cellular origin of hyaluronectin.en
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalJournal of Anatomyen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.