Isolation and characterisation of a hyaluronan binding protein, hyaluronectin, from human placenta and its colocalisation with hyaluronan.

2.50
Hdl Handle:
http://hdl.handle.net/10541/99018
Title:
Isolation and characterisation of a hyaluronan binding protein, hyaluronectin, from human placenta and its colocalisation with hyaluronan.
Authors:
Ponting, Julie M; Kumar, Shant
Abstract:
Hyaluronan (HA) is a major component of the extracellular matrix and is known to influence cell behaviour and to play a role in angiogenesis, morphogenesis and tissue remodelling, although little is known concerning the regulation of these effects. Until now its detection in the placenta has been by indirect methods, which has led to conflicting conclusions as to its distribution and hence its role. Hyaluronectin (HN) is one of a group of proteins with HA binding ability which may regulate the effects of HA. Although nervous tissue HN has been partly characterised with regard to its distribution, structure and biochemistry, little is known about the mesenchymal isoform and its distribution in placenta has not previously been reported. Using specific probes we have characterised the distribution of HA and HN in human placental tissue. At all stages of development studied (8, 10, 12, 30 and 38 wk gestation) HA and HN were unequivocally colocalised, being distributed in the extracellular matrix of stromal tissue of placental villi, chorioallantoic membranes and umbilical cord. Particularly strong immunoreactivity was observed in the villous stroma immediately adjacent to fibrinoid depositions at sites of denudation of the trophoblast layer. Extraction and characterisation of the HN from placental villi have revealed 4 major glycoproteins of 47, 52, 57 and 67 kDa, this being a different pattern and smaller molecular range than observed for the nervous tissue form. This is the first direct demonstration of the presence of HA and HN in the placenta and identifies an abundant new source of mesenchymal HN. The functions of mesenchymal HN are unknown but may include ion exchange, immunosuppression and regulation of the effects of HA in such roles as maintenance of tissue architecture, cell migration and angiogenesis.
Affiliation:
Department of Clinical Research, Christie Hospital, Manchester, UK.
Citation:
Isolation and characterisation of a hyaluronan binding protein, hyaluronectin, from human placenta and its colocalisation with hyaluronan. 1995, 186 ( Pt 1):131-42 J. Anat.
Journal:
Journal of Anatomy
Issue Date:
Feb-1995
URI:
http://hdl.handle.net/10541/99018
PubMed ID:
7544332
Type:
Article
Language:
en
ISSN:
0021-8782
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorPonting, Julie Men
dc.contributor.authorKumar, Shanten
dc.date.accessioned2010-05-17T15:07:43Z-
dc.date.available2010-05-17T15:07:43Z-
dc.date.issued1995-02-
dc.identifier.citationIsolation and characterisation of a hyaluronan binding protein, hyaluronectin, from human placenta and its colocalisation with hyaluronan. 1995, 186 ( Pt 1):131-42 J. Anat.en
dc.identifier.issn0021-8782-
dc.identifier.pmid7544332-
dc.identifier.urihttp://hdl.handle.net/10541/99018-
dc.description.abstractHyaluronan (HA) is a major component of the extracellular matrix and is known to influence cell behaviour and to play a role in angiogenesis, morphogenesis and tissue remodelling, although little is known concerning the regulation of these effects. Until now its detection in the placenta has been by indirect methods, which has led to conflicting conclusions as to its distribution and hence its role. Hyaluronectin (HN) is one of a group of proteins with HA binding ability which may regulate the effects of HA. Although nervous tissue HN has been partly characterised with regard to its distribution, structure and biochemistry, little is known about the mesenchymal isoform and its distribution in placenta has not previously been reported. Using specific probes we have characterised the distribution of HA and HN in human placental tissue. At all stages of development studied (8, 10, 12, 30 and 38 wk gestation) HA and HN were unequivocally colocalised, being distributed in the extracellular matrix of stromal tissue of placental villi, chorioallantoic membranes and umbilical cord. Particularly strong immunoreactivity was observed in the villous stroma immediately adjacent to fibrinoid depositions at sites of denudation of the trophoblast layer. Extraction and characterisation of the HN from placental villi have revealed 4 major glycoproteins of 47, 52, 57 and 67 kDa, this being a different pattern and smaller molecular range than observed for the nervous tissue form. This is the first direct demonstration of the presence of HA and HN in the placenta and identifies an abundant new source of mesenchymal HN. The functions of mesenchymal HN are unknown but may include ion exchange, immunosuppression and regulation of the effects of HA in such roles as maintenance of tissue architecture, cell migration and angiogenesis.en
dc.language.isoenen
dc.subject.meshAntigens, CD44-
dc.subject.meshBlotting, Western-
dc.subject.meshCarrier Proteins-
dc.subject.meshChromatography, Affinity-
dc.subject.meshChromatography, Agarose-
dc.subject.meshExtracellular Matrix-
dc.subject.meshHumans-
dc.subject.meshHyaluronic Acid-
dc.subject.meshImmunoenzyme Techniques-
dc.subject.meshPlacenta-
dc.subject.meshReceptors, Cell Surface-
dc.subject.meshReceptors, Lymphocyte Homing-
dc.subject.meshUmbilical Cord-
dc.titleIsolation and characterisation of a hyaluronan binding protein, hyaluronectin, from human placenta and its colocalisation with hyaluronan.en
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalJournal of Anatomyen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.