Growth factor regulation of proliferation in primary cultures of small intestinal epithelium.

2.50
Hdl Handle:
http://hdl.handle.net/10541/98861
Title:
Growth factor regulation of proliferation in primary cultures of small intestinal epithelium.
Authors:
Booth, Catherine; Evans, Gareth S; Potten, Christopher S
Abstract:
Although the intestinal epithelium is one of the most rapidly renewing tissues, little is known about the major growth factors that control the rate of cell replacement and migration. Recently, a primary culture model has been described for the developing rat small intestinal epithelium, which permits epithelial growth while maintaining interactions with associated stromal cells, thereby possessing several contextual advantages over established cell lines (Evans et al., 1992). We have used this model to begin to determine the factors that may be involved in controlling intestinal epithelial cell proliferation. Under the conditions examined, no single growth factor promoted exclusive proliferation of epithelial cells; stromal cell proliferation was also apparent. The most potent stimulators of epithelial proliferation were insulin and insulin-like growth factor 1 (IGF-1). These factors also appeared to inhibit migration of the epithelial cells. 5-10 ng/ml EGF, 5-20 ng/ml TGF alpha, and 10-20 ng/ml PDGF also slightly increased epithelial cell numbers. Cell proliferation was inhibited by 0.1 ng/ml TGF beta-1. In Dulbecco's modified Eagle's medium (DMEM) containing 0.25 IU/ml insulin, glucose levels of 2-3 g/liter permitted epithelial growth with limited expansion of the stromal cell population. Higher levels of glucose further stimulated the nonepithelial cell types. Transferrin was also a potent stimulator of both cell types.
Affiliation:
Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, United Kingdom.
Citation:
Growth factor regulation of proliferation in primary cultures of small intestinal epithelium. 1995, 31 (3):234-43 In Vitro Cell. Dev. Biol. Anim.
Journal:
In Vitro Cellular & Developmental Biology. Animal
Issue Date:
Mar-1995
URI:
http://hdl.handle.net/10541/98861
DOI:
10.1007/BF02639439
PubMed ID:
7757306
Type:
Article
Language:
en
ISSN:
1071-2690
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBooth, Catherineen
dc.contributor.authorEvans, Gareth Sen
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2010-05-14T15:53:04Z-
dc.date.available2010-05-14T15:53:04Z-
dc.date.issued1995-03-
dc.identifier.citationGrowth factor regulation of proliferation in primary cultures of small intestinal epithelium. 1995, 31 (3):234-43 In Vitro Cell. Dev. Biol. Anim.en
dc.identifier.issn1071-2690-
dc.identifier.pmid7757306-
dc.identifier.doi10.1007/BF02639439-
dc.identifier.urihttp://hdl.handle.net/10541/98861-
dc.description.abstractAlthough the intestinal epithelium is one of the most rapidly renewing tissues, little is known about the major growth factors that control the rate of cell replacement and migration. Recently, a primary culture model has been described for the developing rat small intestinal epithelium, which permits epithelial growth while maintaining interactions with associated stromal cells, thereby possessing several contextual advantages over established cell lines (Evans et al., 1992). We have used this model to begin to determine the factors that may be involved in controlling intestinal epithelial cell proliferation. Under the conditions examined, no single growth factor promoted exclusive proliferation of epithelial cells; stromal cell proliferation was also apparent. The most potent stimulators of epithelial proliferation were insulin and insulin-like growth factor 1 (IGF-1). These factors also appeared to inhibit migration of the epithelial cells. 5-10 ng/ml EGF, 5-20 ng/ml TGF alpha, and 10-20 ng/ml PDGF also slightly increased epithelial cell numbers. Cell proliferation was inhibited by 0.1 ng/ml TGF beta-1. In Dulbecco's modified Eagle's medium (DMEM) containing 0.25 IU/ml insulin, glucose levels of 2-3 g/liter permitted epithelial growth with limited expansion of the stromal cell population. Higher levels of glucose further stimulated the nonepithelial cell types. Transferrin was also a potent stimulator of both cell types.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Division-
dc.subject.meshCells, Cultured-
dc.subject.meshEpidermal Growth Factor-
dc.subject.meshEpithelial Cells-
dc.subject.meshEpithelium-
dc.subject.meshGrowth Substances-
dc.subject.meshHumans-
dc.subject.meshInsulin-
dc.subject.meshInsulin-Like Growth Factor I-
dc.subject.meshIntestine, Small-
dc.subject.meshPlatelet-Derived Growth Factor-
dc.subject.meshRats-
dc.subject.meshRats, Wistar-
dc.subject.meshRecombinant Proteins-
dc.subject.meshTransforming Growth Factor alpha-
dc.subject.meshTransforming Growth Factor beta-
dc.titleGrowth factor regulation of proliferation in primary cultures of small intestinal epithelium.en
dc.typeArticleen
dc.contributor.departmentDepartment of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, United Kingdom.en
dc.identifier.journalIn Vitro Cellular & Developmental Biology. Animalen
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