Changes of mitochondrial mass in the hemopoietic stem cell line FDCP-mix after treatment with etoposide: a correlative study by multiparameter flow cytometry and confocal and electron microscopy.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97948
Title:
Changes of mitochondrial mass in the hemopoietic stem cell line FDCP-mix after treatment with etoposide: a correlative study by multiparameter flow cytometry and confocal and electron microscopy.
Authors:
Reipert, Siegfried; Berry, Jeff; Hughes, Mike F; Hickman, J A; Allen, Terence D
Abstract:
FDCP-Mix, a pluripotent murine hemopoietic stem cell line which undergoes typical internucleosomal cleavage of DNA when induced to apoptosis by either drugs or withdrawal of growth factor (interleukin-3) was studied after treatment with the topoisomerase II inhibitor etoposide (0.5-4 microM). An increase in autolytic activity was the major early morphological change within the cytoplasm, with mitochondria as the main target for autolytic digestion. Despite this macroautophagy, thin sections showed a high number of mitochondria, suggesting mitochondrial proliferation as a result of drug treatment. This observation of an increase in the number of mitochondria was confirmed by flow cytometric studies of mitochondrial overall mass. Multiparameter flow cytometry of cells double stained with propidium iodide and nonyl-acridine orange gave an accurate assay for mitochondrial mass in relation to cell cycle stages. The increase in mitochondrial mass was found in all cell cycle stages. The results suggest a drug-induced proliferation of mitochondria separate from the processes involved in the doubling of mitochondrial mass during the cell cycle and a decline of mitochondria in the later stages of apoptosis.
Affiliation:
Department of Structural Cell Biology, Paterson Institute for Cancer Research & Christie Hospital, Manchester, United Kingdom.
Citation:
Changes of mitochondrial mass in the hemopoietic stem cell line FDCP-mix after treatment with etoposide: a correlative study by multiparameter flow cytometry and confocal and electron microscopy. 1995, 221 (2):281-8 Exp. Cell Res.
Journal:
Experimental Cell Research
Issue Date:
Dec-1995
URI:
http://hdl.handle.net/10541/97948
DOI:
10.1006/excr.1995.1376
PubMed ID:
7493625
Type:
Article
Language:
en
ISSN:
0014-4827
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorReipert, Siegfrieden
dc.contributor.authorBerry, Jeffen
dc.contributor.authorHughes, Mike Fen
dc.contributor.authorHickman, J Aen
dc.contributor.authorAllen, Terence Den
dc.date.accessioned2010-05-05T12:42:29Z-
dc.date.available2010-05-05T12:42:29Z-
dc.date.issued1995-12-
dc.identifier.citationChanges of mitochondrial mass in the hemopoietic stem cell line FDCP-mix after treatment with etoposide: a correlative study by multiparameter flow cytometry and confocal and electron microscopy. 1995, 221 (2):281-8 Exp. Cell Res.en
dc.identifier.issn0014-4827-
dc.identifier.pmid7493625-
dc.identifier.doi10.1006/excr.1995.1376-
dc.identifier.urihttp://hdl.handle.net/10541/97948-
dc.description.abstractFDCP-Mix, a pluripotent murine hemopoietic stem cell line which undergoes typical internucleosomal cleavage of DNA when induced to apoptosis by either drugs or withdrawal of growth factor (interleukin-3) was studied after treatment with the topoisomerase II inhibitor etoposide (0.5-4 microM). An increase in autolytic activity was the major early morphological change within the cytoplasm, with mitochondria as the main target for autolytic digestion. Despite this macroautophagy, thin sections showed a high number of mitochondria, suggesting mitochondrial proliferation as a result of drug treatment. This observation of an increase in the number of mitochondria was confirmed by flow cytometric studies of mitochondrial overall mass. Multiparameter flow cytometry of cells double stained with propidium iodide and nonyl-acridine orange gave an accurate assay for mitochondrial mass in relation to cell cycle stages. The increase in mitochondrial mass was found in all cell cycle stages. The results suggest a drug-induced proliferation of mitochondria separate from the processes involved in the doubling of mitochondrial mass during the cell cycle and a decline of mitochondria in the later stages of apoptosis.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAcridine Orange-
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshAutolysis-
dc.subject.meshCell Line-
dc.subject.meshColoring Agents-
dc.subject.meshCytoplasm-
dc.subject.meshDNA Topoisomerases, Type II-
dc.subject.meshEnzyme Inhibitors-
dc.subject.meshEtoposide-
dc.subject.meshFlow Cytometry-
dc.subject.meshG2 Phase-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshInterleukin-3-
dc.subject.meshMice-
dc.subject.meshMicroscopy, Confocal-
dc.subject.meshMicroscopy, Electron-
dc.subject.meshMitochondria-
dc.subject.meshPropidium-
dc.titleChanges of mitochondrial mass in the hemopoietic stem cell line FDCP-mix after treatment with etoposide: a correlative study by multiparameter flow cytometry and confocal and electron microscopy.en
dc.typeArticleen
dc.contributor.departmentDepartment of Structural Cell Biology, Paterson Institute for Cancer Research & Christie Hospital, Manchester, United Kingdom.en
dc.identifier.journalExperimental Cell Researchen

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