Establishment of the marker order pter-NRAS-NGFB-D1S189-D1S252-D1S440-D1S453-D1S514-CEN-D1S442-D1S498-qte r in relation to the centromere on human chromosome 1.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97943
Title:
Establishment of the marker order pter-NRAS-NGFB-D1S189-D1S252-D1S440-D1S453-D1S514-CEN-D1S442-D1S498-qte r in relation to the centromere on human chromosome 1.
Authors:
Hoggard, Nigel; Brintnell, Bill; Hey, Yvonne; Jones, David; Weissenbach, J; Mitchell, Erika L D; Varley, Jennifer
Abstract:
Recent developments in genetic linkage mapping of the human genome have generated a large number of short tandem repeat polymorphic markers (Weissenbach et al. 1992, Gyapay et al. 1994), and eventual integration of these markers into a physical map is a logical progression. A number of Généthon microsatellite (CA repeat) markers have been provisionally localized to 1p13, but their exact position with respect to other sequences is unknown. In order to confirm the order of these markers and their position with respect to known genes within 1p13 and the centromere, we have isolated yeast artificial chromosomes (YACs) corresponding to the markers and have carried out double and triple fluorescence in situ hybridization (FISH) studies. Knowledge of both the order of microsatellite markers and their integration with a physical map of known genes can be an essential component in analysis of disease loci such as human cancer, where regions of chromosomes showing high levels of loss of heterozygosity need to be mapped in detail.
Affiliation:
CRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.
Citation:
Establishment of the marker order pter-NRAS-NGFB-D1S189-D1S252-D1S440-D1S453-D1S514-CEN-D1S442-D1S498-qte r in relation to the centromere on human chromosome 1. 1995, 3 (2):137-8 Chromosome Res.
Journal:
Chromosome Research
Issue Date:
Mar-1995
URI:
http://hdl.handle.net/10541/97943
DOI:
10.1007/BF00710677
PubMed ID:
7749565
Type:
Article
Language:
en
ISSN:
0967-3849
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHoggard, Nigelen
dc.contributor.authorBrintnell, Billen
dc.contributor.authorHey, Yvonneen
dc.contributor.authorJones, Daviden
dc.contributor.authorWeissenbach, Jen
dc.contributor.authorMitchell, Erika L Den
dc.contributor.authorVarley, Jenniferen
dc.date.accessioned2010-05-05T12:22:59Z-
dc.date.available2010-05-05T12:22:59Z-
dc.date.issued1995-03-
dc.identifier.citationEstablishment of the marker order pter-NRAS-NGFB-D1S189-D1S252-D1S440-D1S453-D1S514-CEN-D1S442-D1S498-qte r in relation to the centromere on human chromosome 1. 1995, 3 (2):137-8 Chromosome Res.en
dc.identifier.issn0967-3849-
dc.identifier.pmid7749565-
dc.identifier.doi10.1007/BF00710677-
dc.identifier.urihttp://hdl.handle.net/10541/97943-
dc.description.abstractRecent developments in genetic linkage mapping of the human genome have generated a large number of short tandem repeat polymorphic markers (Weissenbach et al. 1992, Gyapay et al. 1994), and eventual integration of these markers into a physical map is a logical progression. A number of Généthon microsatellite (CA repeat) markers have been provisionally localized to 1p13, but their exact position with respect to other sequences is unknown. In order to confirm the order of these markers and their position with respect to known genes within 1p13 and the centromere, we have isolated yeast artificial chromosomes (YACs) corresponding to the markers and have carried out double and triple fluorescence in situ hybridization (FISH) studies. Knowledge of both the order of microsatellite markers and their integration with a physical map of known genes can be an essential component in analysis of disease loci such as human cancer, where regions of chromosomes showing high levels of loss of heterozygosity need to be mapped in detail.en
dc.language.isoenen
dc.subject.meshCentromere-
dc.subject.meshChromosome Mapping-
dc.subject.meshChromosomes, Artificial, Yeast-
dc.subject.meshChromosomes, Human, Pair 1-
dc.subject.meshDNA, Satellite-
dc.subject.meshGenetic Markers-
dc.subject.meshHumans-
dc.subject.meshIn Situ Hybridization, Fluorescence-
dc.subject.meshLinkage (Genetics)-
dc.subject.meshMale-
dc.titleEstablishment of the marker order pter-NRAS-NGFB-D1S189-D1S252-D1S440-D1S453-D1S514-CEN-D1S442-D1S498-qte r in relation to the centromere on human chromosome 1.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalChromosome Researchen

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