Confirmation of the copy number of chromosome 1 in interphase nuclei from paraffin sections of breast tumours by fluorescence in situ hybridization.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97942
Title:
Confirmation of the copy number of chromosome 1 in interphase nuclei from paraffin sections of breast tumours by fluorescence in situ hybridization.
Authors:
Loupart, M L; Walker, R; Brammar, W; Varley, Jennifer
Abstract:
Fluorescence in situ hybridization (FISH) was used to establish the copy number of chromosome 1 in a set of nine breast tumours in which the chromosome had previously been shown to have undergone a variety of rearrangements by loss of heterozygosity studies. In each case, FISH with satellite III DNA from chromosome 1q12 confirmed the results obtained by Southern hybridization. Importantly, in all five cases with rearrangements thought not to involve the centromeric region, FISH showed that the events had not disrupted the gross chromosome structure. This study highlights the potential of using the two techniques together to obtain a clearer picture of both large- and small-scale alterations to chromosomes in solid tumours.
Affiliation:
ICI/University Joint Laboratory, University of Leicster, UK.
Citation:
Confirmation of the copy number of chromosome 1 in interphase nuclei from paraffin sections of breast tumours by fluorescence in situ hybridization. 1995, 3 (7):410-6 Chromosome Res.
Journal:
Chromosome Research
Issue Date:
Nov-1995
URI:
http://hdl.handle.net/10541/97942
DOI:
10.1007/BF00713890
PubMed ID:
8528585
Type:
Article
Language:
en
ISSN:
0967-3849
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLoupart, M Len
dc.contributor.authorWalker, Ren
dc.contributor.authorBrammar, Wen
dc.contributor.authorVarley, Jenniferen
dc.date.accessioned2010-05-05T12:19:16Z-
dc.date.available2010-05-05T12:19:16Z-
dc.date.issued1995-11-
dc.identifier.citationConfirmation of the copy number of chromosome 1 in interphase nuclei from paraffin sections of breast tumours by fluorescence in situ hybridization. 1995, 3 (7):410-6 Chromosome Res.en
dc.identifier.issn0967-3849-
dc.identifier.pmid8528585-
dc.identifier.doi10.1007/BF00713890-
dc.identifier.urihttp://hdl.handle.net/10541/97942-
dc.description.abstractFluorescence in situ hybridization (FISH) was used to establish the copy number of chromosome 1 in a set of nine breast tumours in which the chromosome had previously been shown to have undergone a variety of rearrangements by loss of heterozygosity studies. In each case, FISH with satellite III DNA from chromosome 1q12 confirmed the results obtained by Southern hybridization. Importantly, in all five cases with rearrangements thought not to involve the centromeric region, FISH showed that the events had not disrupted the gross chromosome structure. This study highlights the potential of using the two techniques together to obtain a clearer picture of both large- and small-scale alterations to chromosomes in solid tumours.en
dc.language.isoenen
dc.subjectBreast Canceren
dc.subject.meshAlleles-
dc.subject.meshBlotting, Southern-
dc.subject.meshBreast Neoplasms-
dc.subject.meshCentromere-
dc.subject.meshChromosome Aberrations-
dc.subject.meshChromosomes, Human, Pair 1-
dc.subject.meshDNA Probes-
dc.subject.meshDNA, Satellite-
dc.subject.meshFemale-
dc.subject.meshHeterozygote-
dc.subject.meshHumans-
dc.subject.meshIn Situ Hybridization, Fluorescence-
dc.subject.meshMicroscopy, Fluorescence-
dc.subject.meshParaffin Embedding-
dc.subject.meshTrisomy-
dc.titleConfirmation of the copy number of chromosome 1 in interphase nuclei from paraffin sections of breast tumours by fluorescence in situ hybridization.en
dc.typeArticleen
dc.contributor.departmentICI/University Joint Laboratory, University of Leicster, UK.en
dc.identifier.journalChromosome Researchen

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