Confirmation of the copy number of chromosome 1 in interphase nuclei from paraffin sections of breast tumours by fluorescence in situ hybridization.
Affiliation
ICI/University Joint Laboratory, University of Leicster, UK.Issue Date
1995-11
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Fluorescence in situ hybridization (FISH) was used to establish the copy number of chromosome 1 in a set of nine breast tumours in which the chromosome had previously been shown to have undergone a variety of rearrangements by loss of heterozygosity studies. In each case, FISH with satellite III DNA from chromosome 1q12 confirmed the results obtained by Southern hybridization. Importantly, in all five cases with rearrangements thought not to involve the centromeric region, FISH showed that the events had not disrupted the gross chromosome structure. This study highlights the potential of using the two techniques together to obtain a clearer picture of both large- and small-scale alterations to chromosomes in solid tumours.Citation
Confirmation of the copy number of chromosome 1 in interphase nuclei from paraffin sections of breast tumours by fluorescence in situ hybridization. 1995, 3 (7):410-6 Chromosome Res.Journal
Chromosome ResearchDOI
10.1007/BF00713890PubMed ID
8528585Type
ArticleLanguage
enISSN
0967-3849ae974a485f413a2113503eed53cd6c53
10.1007/BF00713890
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