Testicular toxicity of the transferrin binding radionuclide 114mIn in adult and neonatal rats.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97936
Title:
Testicular toxicity of the transferrin binding radionuclide 114mIn in adult and neonatal rats.
Authors:
Hoyes, Katherine P; Johnson, C; Johnston, R E; Lendon, R G; Hendry, Jolyon H; Sharma, H L; Morris, Ian D
Abstract:
Adult (70 d) and neonatal (7 d) male rats were dosed (i.p.) with 37 MBq/kg (1 mCi/kg; approximately 1 microgram elemental indium/kg) 114mIn, a transferrin-binding radionuclide. In adults, approximately 0.25% of the injected activity localised within the testis by 48 h postinjection and remained constant for up to 63 d. In neonates, 0.06% of the activity was in the testis by 48 h, and this declined such that by 63 d only 0.03% remained. At 63 d, treated rats had reduced sperm head counts and abnormal testicular histology that was more marked in animals dosed as adults than as neonates. In vitro, uptake of 114mIn into seminiferous tubules isolated from 7-, 20-, or 70-d-old rats was compared with that of 125I. Both radionuclides were readily accumulated by the tubules. Whilst 114In uptake into 20- and 70-d tubules was inhibited by excess transferrin, uptake into 7-d tubules was unchanged. 125I uptake was not affected by excess transferrin. These data support the contention that some radionuclides may cross the blood-testis barrier by utilisation of the physiologic iron-transferrin pathway, which may lead to greater testicular damage in adult compared to neonatal animals.
Affiliation:
Cancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, UK.
Citation:
Testicular toxicity of the transferrin binding radionuclide 114mIn in adult and neonatal rats., 9 (3):297-305 Reprod. Toxicol.
Journal:
Reproductive Toxicology
Issue Date:
5-May-2010
URI:
http://hdl.handle.net/10541/97936
DOI:
10.1016/0890-6238(95)00012-Y
PubMed ID:
7579915
Type:
Article
Language:
en
ISSN:
0890-6238
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHoyes, Katherine Pen
dc.contributor.authorJohnson, Cen
dc.contributor.authorJohnston, R Een
dc.contributor.authorLendon, R Gen
dc.contributor.authorHendry, Jolyon Hen
dc.contributor.authorSharma, H Len
dc.contributor.authorMorris, Ian Den
dc.date.accessioned2010-05-05T10:40:26Z-
dc.date.available2010-05-05T10:40:26Z-
dc.date.issued2010-05-05T10:40:26Z-
dc.identifier.citationTesticular toxicity of the transferrin binding radionuclide 114mIn in adult and neonatal rats., 9 (3):297-305 Reprod. Toxicol.en
dc.identifier.issn0890-6238-
dc.identifier.pmid7579915-
dc.identifier.doi10.1016/0890-6238(95)00012-Y-
dc.identifier.urihttp://hdl.handle.net/10541/97936-
dc.description.abstractAdult (70 d) and neonatal (7 d) male rats were dosed (i.p.) with 37 MBq/kg (1 mCi/kg; approximately 1 microgram elemental indium/kg) 114mIn, a transferrin-binding radionuclide. In adults, approximately 0.25% of the injected activity localised within the testis by 48 h postinjection and remained constant for up to 63 d. In neonates, 0.06% of the activity was in the testis by 48 h, and this declined such that by 63 d only 0.03% remained. At 63 d, treated rats had reduced sperm head counts and abnormal testicular histology that was more marked in animals dosed as adults than as neonates. In vitro, uptake of 114mIn into seminiferous tubules isolated from 7-, 20-, or 70-d-old rats was compared with that of 125I. Both radionuclides were readily accumulated by the tubules. Whilst 114In uptake into 20- and 70-d tubules was inhibited by excess transferrin, uptake into 7-d tubules was unchanged. 125I uptake was not affected by excess transferrin. These data support the contention that some radionuclides may cross the blood-testis barrier by utilisation of the physiologic iron-transferrin pathway, which may lead to greater testicular damage in adult compared to neonatal animals.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshAnimals, Newborn-
dc.subject.meshBody Weight-
dc.subject.meshIndium Radioisotopes-
dc.subject.meshMale-
dc.subject.meshOrgan Size-
dc.subject.meshProtein Binding-
dc.subject.meshRats-
dc.subject.meshRats, Sprague-Dawley-
dc.subject.meshSeminiferous Tubules-
dc.subject.meshSperm Count-
dc.subject.meshSperm Head-
dc.subject.meshTestis-
dc.subject.meshTissue Distribution-
dc.subject.meshTransferrin-
dc.titleTesticular toxicity of the transferrin binding radionuclide 114mIn in adult and neonatal rats.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalReproductive Toxicologyen

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