Discordant regulation of SCL/TAL-1 mRNA and protein during erythroid differentiation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97776
Title:
Discordant regulation of SCL/TAL-1 mRNA and protein during erythroid differentiation.
Authors:
Murrell, A M; Bockamp, E O; Göttgens, B; Chan, Y S; Cross, Michael A; Heyworth, Clare M; Green, A R
Abstract:
The SCL/TAL1 gene was originally identified by virtue of its rearrangement and transcriptional activation in patients with T cell acute lymphoblastic leukaemia. It encodes a helix-loop-helix transcription factor, is not normally expressed in T cells, but is expressed in erythroid, mast, megakaryocytic and progenitor cells. Over-expression of sense and antisense constructs have implicated SCL as a positive regulator of erythroid differentiation. In addition we have previously shown that SCL mRNA levels undergo biphasic modulation during induced erythroid differentiation of murine erythroleukaemia (MEL) cells with a transient early fall followed by a late rise. In this paper we have studied expression of the SCL protein during erythroid differentiation and also the molecular basis for the raised SCL mRNA levels that accompany erythroid differentiation. We have generated an anti-SCL antiserum and used it to demonstrate that an early transient fall in SCL protein does not occur during induced differentiation of MEL cells. Furthermore SCL protein levels underwent a late fall in three different models of erythroid differentiation and in two models of myeloid differentiation. The fall in SCL protein levels during induced erythroid differentiation contrasted with the concomitant marked rise in SCL mRNA levels. These observations have significant implications for the mechanism by which SCL may regulate erythropoiesis. In addition we have demonstrated that the stability of SCL mRNA was only marginally enhanced during erythroid differentiation of MEL cells, whereas the activity of a luciferase reporter construct driven by the SCL promoter was increased 11- to 17-fold. Up-regulation of transcription therefore accounted for most of the increase in SCL mRNA levels during erythroid differentiation.
Affiliation:
University of Cambridge, Department of Haematology, MRC Centre, UK.
Citation:
Discordant regulation of SCL/TAL-1 mRNA and protein during erythroid differentiation. 1995, 11 (1):131-9 Oncogene
Journal:
Oncogene
Issue Date:
6-Jul-1995
URI:
http://hdl.handle.net/10541/97776
PubMed ID:
7624120
Type:
Article
Language:
en
ISSN:
0950-9232
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMurrell, A Men
dc.contributor.authorBockamp, E Oen
dc.contributor.authorGöttgens, Ben
dc.contributor.authorChan, Y Sen
dc.contributor.authorCross, Michael Aen
dc.contributor.authorHeyworth, Clare Men
dc.contributor.authorGreen, A Ren
dc.date.accessioned2010-04-30T15:42:27Z-
dc.date.available2010-04-30T15:42:27Z-
dc.date.issued1995-07-06-
dc.identifier.citationDiscordant regulation of SCL/TAL-1 mRNA and protein during erythroid differentiation. 1995, 11 (1):131-9 Oncogeneen
dc.identifier.issn0950-9232-
dc.identifier.pmid7624120-
dc.identifier.urihttp://hdl.handle.net/10541/97776-
dc.description.abstractThe SCL/TAL1 gene was originally identified by virtue of its rearrangement and transcriptional activation in patients with T cell acute lymphoblastic leukaemia. It encodes a helix-loop-helix transcription factor, is not normally expressed in T cells, but is expressed in erythroid, mast, megakaryocytic and progenitor cells. Over-expression of sense and antisense constructs have implicated SCL as a positive regulator of erythroid differentiation. In addition we have previously shown that SCL mRNA levels undergo biphasic modulation during induced erythroid differentiation of murine erythroleukaemia (MEL) cells with a transient early fall followed by a late rise. In this paper we have studied expression of the SCL protein during erythroid differentiation and also the molecular basis for the raised SCL mRNA levels that accompany erythroid differentiation. We have generated an anti-SCL antiserum and used it to demonstrate that an early transient fall in SCL protein does not occur during induced differentiation of MEL cells. Furthermore SCL protein levels underwent a late fall in three different models of erythroid differentiation and in two models of myeloid differentiation. The fall in SCL protein levels during induced erythroid differentiation contrasted with the concomitant marked rise in SCL mRNA levels. These observations have significant implications for the mechanism by which SCL may regulate erythropoiesis. In addition we have demonstrated that the stability of SCL mRNA was only marginally enhanced during erythroid differentiation of MEL cells, whereas the activity of a luciferase reporter construct driven by the SCL promoter was increased 11- to 17-fold. Up-regulation of transcription therefore accounted for most of the increase in SCL mRNA levels during erythroid differentiation.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshAntibodies-
dc.subject.meshAntibody Specificity-
dc.subject.meshBasic Helix-Loop-Helix Transcription Factors-
dc.subject.meshCell Line-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshErythropoiesis-
dc.subject.meshGene Expression Regulation-
dc.subject.meshMice-
dc.subject.meshProto-Oncogene Proteins-
dc.subject.meshRNA, Messenger-
dc.subject.meshTranscription Factors-
dc.subject.meshTranscription, Genetic-
dc.titleDiscordant regulation of SCL/TAL-1 mRNA and protein during erythroid differentiation.en
dc.typeArticleen
dc.contributor.departmentUniversity of Cambridge, Department of Haematology, MRC Centre, UK.en
dc.identifier.journalOncogeneen

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