The Epstein-Barr virus encoded cytokine viral interleukin-10 enhances transformation of human B lymphocytes.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97769
Title:
The Epstein-Barr virus encoded cytokine viral interleukin-10 enhances transformation of human B lymphocytes.
Authors:
Stuart, Amanda D; Stewart, James P; Arrand, John R; Mackett, Mike
Abstract:
In vitro infection of human B lymphocytes with Epstein-Barr virus (EBV) results in their growth transformation and establishment of immortalised lymphoblastoid cell lines. The virus was recently found to encode a homologue of the pleitropic cytokine interleukin-10 (IL-10), which has wide ranging effects on the immune system. We have investigated the effect of this virally encoded growth factor on the ability of EBV to immortalize B lymphocytes from tonsils and from adult and neonatal blood. Recombinant viral interleukin-10 (vIL-10) was found to increase dramatically the growth transformation of B cells from all three populations infected with either the highly transforming type 1 strain B95-8 or the less efficient type 2 strain BL16. This striking enhancement of transforming ability in the presence of viral IL-10 may be in part due to increased viability of the B cells during infection and decreased levels of interferon-gamma, a cytokine known to inhibit EBV transformation. Thus viral IL-10 influences a number of cell types of the immune system to allow the enhanced outgrowth of EBV transformed cells.
Affiliation:
Department of Molecular Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Withington, Manchester.
Citation:
The Epstein-Barr virus encoded cytokine viral interleukin-10 enhances transformation of human B lymphocytes. 1995, 11 (9):1711-9 Oncogene
Journal:
Oncogene
Issue Date:
2-Nov-1995
URI:
http://hdl.handle.net/10541/97769
PubMed ID:
7478598
Type:
Article
Language:
en
ISSN:
0950-9232
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorStuart, Amanda Den
dc.contributor.authorStewart, James Pen
dc.contributor.authorArrand, John Ren
dc.contributor.authorMackett, Mikeen
dc.date.accessioned2010-04-30T15:47:23Z-
dc.date.available2010-04-30T15:47:23Z-
dc.date.issued1995-11-02-
dc.identifier.citationThe Epstein-Barr virus encoded cytokine viral interleukin-10 enhances transformation of human B lymphocytes. 1995, 11 (9):1711-9 Oncogeneen
dc.identifier.issn0950-9232-
dc.identifier.pmid7478598-
dc.identifier.urihttp://hdl.handle.net/10541/97769-
dc.description.abstractIn vitro infection of human B lymphocytes with Epstein-Barr virus (EBV) results in their growth transformation and establishment of immortalised lymphoblastoid cell lines. The virus was recently found to encode a homologue of the pleitropic cytokine interleukin-10 (IL-10), which has wide ranging effects on the immune system. We have investigated the effect of this virally encoded growth factor on the ability of EBV to immortalize B lymphocytes from tonsils and from adult and neonatal blood. Recombinant viral interleukin-10 (vIL-10) was found to increase dramatically the growth transformation of B cells from all three populations infected with either the highly transforming type 1 strain B95-8 or the less efficient type 2 strain BL16. This striking enhancement of transforming ability in the presence of viral IL-10 may be in part due to increased viability of the B cells during infection and decreased levels of interferon-gamma, a cytokine known to inhibit EBV transformation. Thus viral IL-10 influences a number of cell types of the immune system to allow the enhanced outgrowth of EBV transformed cells.en
dc.language.isoenen
dc.subjectFoetal Blooden
dc.subject.meshAdult-
dc.subject.meshB-Lymphocytes-
dc.subject.meshCell Line, Transformed-
dc.subject.meshCell Survival-
dc.subject.meshCell Transformation, Viral-
dc.subject.meshFetal Blood-
dc.subject.meshHerpesvirus 4, Human-
dc.subject.meshHumans-
dc.subject.meshInfant, Newborn-
dc.subject.meshInterleukin-10-
dc.subject.meshLymphocyte Activation-
dc.subject.meshOpen Reading Frames-
dc.subject.meshPalatine Tonsil-
dc.subject.meshSpecies Specificity-
dc.titleThe Epstein-Barr virus encoded cytokine viral interleukin-10 enhances transformation of human B lymphocytes.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Withington, Manchester.en
dc.identifier.journalOncogeneen
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