2.50
Hdl Handle:
http://hdl.handle.net/10541/97751
Title:
Expression of Id2 and Id3 mRNA in human lymphocytes.
Authors:
Ishiguro, A; Spirin, K; Shiohara, M; Tobler, A; Norton, John D; Rigolet, M; Shimbo, T; Koeffler, H P
Abstract:
Helix-loop-helix (HLH) transcription factors are involved in cellular growth and differentiation. The Id (inhibitor of DNA binding and differentiation) HLH proteins, in a dominantly negative fashion, regulate transcriptional activities of basic HLH proteins. We examined by northern hybridization the expression of Id2 and Id3 mRNA in human leukemia/lymphoma lines and patient samples, as well as resting and activated normal human lymphocytes from peripheral blood (PBL). The Id2 mRNA was abundantly expressed in 5/12 T-cell and 3/4 B-cell lines, and Id3 mRNA was detected in 4/12 T-cell and 3/4 B-cell lines. Interestingly, Id2, but not Id3, mRNA was strongly expressed in 4/5 T-cell lines infected with human T-cell leukemia virus type I (HTLV-I) (ATL-1k, MT-2, S-LB1) and type II (Mo). Another unexpected finding was that T-cell leukemias and T-cell lines often expressed either Id2 or Id3 mRNA. In addition, resting PBL constitutively expressed prominent levels of Id2 mRNA, but not Id3 mRNA. Upon PHA-stimulation, Id2 expression decreased and Id3 levels increased with biphasic kinetics. Taken together, our studies revealed three unexpected findings which require further analysis: (1) expression of Id2 mRNA is often associated with lymphocytic transformation by HTLV-I or -II; (2) T-cells usually express either Id2 or Id3 mRNA, but B-cells often express both simultaneously; (3) non-dividing, normal PBL express high levels of Id2 and no Id3 mRNA; and with the onset of cellular proliferation, levels of Id2 mRNA decrease while levels of Id3 mRNA increase, suggesting that regulation of expression of these closely related genes is disparate.
Affiliation:
Department of Medicine, Cedars-Sinai Medical Center, UCLA School of Medicine 90048, USA.
Citation:
Expression of Id2 and Id3 mRNA in human lymphocytes. 1995, 19 (12):989-96 Leuk. Res.
Journal:
Leukemia Research
Issue Date:
Dec-1995
URI:
http://hdl.handle.net/10541/97751
DOI:
10.1016/0145-2126(95)00084-4
PubMed ID:
8632670
Type:
Article
Language:
en
ISSN:
0145-2126
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorIshiguro, Aen
dc.contributor.authorSpirin, Ken
dc.contributor.authorShiohara, Men
dc.contributor.authorTobler, Aen
dc.contributor.authorNorton, John Den
dc.contributor.authorRigolet, Men
dc.contributor.authorShimbo, Ten
dc.contributor.authorKoeffler, H Pen
dc.date.accessioned2010-04-30T15:35:23Z-
dc.date.available2010-04-30T15:35:23Z-
dc.date.issued1995-12-
dc.identifier.citationExpression of Id2 and Id3 mRNA in human lymphocytes. 1995, 19 (12):989-96 Leuk. Res.en
dc.identifier.issn0145-2126-
dc.identifier.pmid8632670-
dc.identifier.doi10.1016/0145-2126(95)00084-4-
dc.identifier.urihttp://hdl.handle.net/10541/97751-
dc.description.abstractHelix-loop-helix (HLH) transcription factors are involved in cellular growth and differentiation. The Id (inhibitor of DNA binding and differentiation) HLH proteins, in a dominantly negative fashion, regulate transcriptional activities of basic HLH proteins. We examined by northern hybridization the expression of Id2 and Id3 mRNA in human leukemia/lymphoma lines and patient samples, as well as resting and activated normal human lymphocytes from peripheral blood (PBL). The Id2 mRNA was abundantly expressed in 5/12 T-cell and 3/4 B-cell lines, and Id3 mRNA was detected in 4/12 T-cell and 3/4 B-cell lines. Interestingly, Id2, but not Id3, mRNA was strongly expressed in 4/5 T-cell lines infected with human T-cell leukemia virus type I (HTLV-I) (ATL-1k, MT-2, S-LB1) and type II (Mo). Another unexpected finding was that T-cell leukemias and T-cell lines often expressed either Id2 or Id3 mRNA. In addition, resting PBL constitutively expressed prominent levels of Id2 mRNA, but not Id3 mRNA. Upon PHA-stimulation, Id2 expression decreased and Id3 levels increased with biphasic kinetics. Taken together, our studies revealed three unexpected findings which require further analysis: (1) expression of Id2 mRNA is often associated with lymphocytic transformation by HTLV-I or -II; (2) T-cells usually express either Id2 or Id3 mRNA, but B-cells often express both simultaneously; (3) non-dividing, normal PBL express high levels of Id2 and no Id3 mRNA; and with the onset of cellular proliferation, levels of Id2 mRNA decrease while levels of Id3 mRNA increase, suggesting that regulation of expression of these closely related genes is disparate.en
dc.language.isoenen
dc.subjectLeukaemiaen
dc.subjectCancer Proteinsen
dc.subject.meshB-Lymphocytes-
dc.subject.meshBlotting, Northern-
dc.subject.meshCell Transformation, Viral-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshHelix-Loop-Helix Motifs-
dc.subject.meshHuman T-lymphotropic virus 1-
dc.subject.meshHuman T-lymphotropic virus 2-
dc.subject.meshHumans-
dc.subject.meshInhibitor of Differentiation Protein 2-
dc.subject.meshInhibitor of Differentiation Proteins-
dc.subject.meshLeukemia-
dc.subject.meshLymphocyte Activation-
dc.subject.meshLymphocytes-
dc.subject.meshLymphoma-
dc.subject.meshNeoplasm Proteins-
dc.subject.meshRNA, Messenger-
dc.subject.meshRepressor Proteins-
dc.subject.meshT-Lymphocytes-
dc.subject.meshTranscription Factors-
dc.titleExpression of Id2 and Id3 mRNA in human lymphocytes.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medicine, Cedars-Sinai Medical Center, UCLA School of Medicine 90048, USA.en
dc.identifier.journalLeukemia Researchen

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