2.50
Hdl Handle:
http://hdl.handle.net/10541/97749
Title:
A radiation hybrid panel for human chromosome 6q.
Authors:
Orphanos, Vassilis; Greaves, Martin J; Santibanez-Koref, Mauro F; Fox, M; Edwards, Y H; Boyle, John M
Abstract:
A panel of 63 radiation-reduced hybrids has been derived from a mouse cell line containing a neo-marked human Chromosome (Chr) 6, primarily to provide a resource for higher resolution localization of new markers. Hybrids were generated with radiation doses of 40-400 Gy, selected in G418, and were shown by PCR to contain the neo gene. PCR was also used to score the retention of 15 loci that map from 6q13 to q25.2 of the current consensus map, plus six other loci assigned to 6q26-q27. An average retention frequency of 27.8% was observed, with the highest frequencies at D6S313 and D6S280 (63.5%) located near the centromere at 6q13, and at D6S283 (68.5%) at 6q16.3-q21, presumably close to the neo integration site. Lowest frequencies (4.8%) were observed for telomeric markers. All markers segregated independently except D6S297 and D6S193. Agreement and some improvement to the current consensus map of 6q was made by mapping 12 loci by the non-parametric statistical method of Falk. In addition, deletion mapping with informative hybrids allowed the ordering of six loci from 6q26 to q27 and permitted some integration of maps of this region.
Affiliation:
Cancer Research Campaign Department of Cancer Genetics, Christie CRC Research Centre, Manchester, UK.
Citation:
A radiation hybrid panel for human chromosome 6q. 1995, 6 (4):285-90 Mamm. Genome
Journal:
Mammalian Genome
Issue Date:
Apr-1995
URI:
http://hdl.handle.net/10541/97749
DOI:
10.1007/BF00352418
PubMed ID:
7613036
Type:
Article
Language:
en
ISSN:
0938-8990
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorOrphanos, Vassilisen
dc.contributor.authorGreaves, Martin Jen
dc.contributor.authorSantibanez-Koref, Mauro Fen
dc.contributor.authorFox, Men
dc.contributor.authorEdwards, Y Hen
dc.contributor.authorBoyle, John Men
dc.date.accessioned2010-04-30T15:32:04Z-
dc.date.available2010-04-30T15:32:04Z-
dc.date.issued1995-04-
dc.identifier.citationA radiation hybrid panel for human chromosome 6q. 1995, 6 (4):285-90 Mamm. Genomeen
dc.identifier.issn0938-8990-
dc.identifier.pmid7613036-
dc.identifier.doi10.1007/BF00352418-
dc.identifier.urihttp://hdl.handle.net/10541/97749-
dc.description.abstractA panel of 63 radiation-reduced hybrids has been derived from a mouse cell line containing a neo-marked human Chromosome (Chr) 6, primarily to provide a resource for higher resolution localization of new markers. Hybrids were generated with radiation doses of 40-400 Gy, selected in G418, and were shown by PCR to contain the neo gene. PCR was also used to score the retention of 15 loci that map from 6q13 to q25.2 of the current consensus map, plus six other loci assigned to 6q26-q27. An average retention frequency of 27.8% was observed, with the highest frequencies at D6S313 and D6S280 (63.5%) located near the centromere at 6q13, and at D6S283 (68.5%) at 6q16.3-q21, presumably close to the neo integration site. Lowest frequencies (4.8%) were observed for telomeric markers. All markers segregated independently except D6S297 and D6S193. Agreement and some improvement to the current consensus map of 6q was made by mapping 12 loci by the non-parametric statistical method of Falk. In addition, deletion mapping with informative hybrids allowed the ordering of six loci from 6q26 to q27 and permitted some integration of maps of this region.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshCell Line-
dc.subject.meshChromosome Mapping-
dc.subject.meshChromosomes, Human, Pair 6-
dc.subject.meshGenetic Markers-
dc.subject.meshHumans-
dc.subject.meshIn Situ Hybridization, Fluorescence-
dc.subject.meshKanamycin Kinase-
dc.subject.meshMice-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPhosphotransferases (Alcohol Group Acceptor)-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshRadiation Chimera-
dc.titleA radiation hybrid panel for human chromosome 6q.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Cancer Genetics, Christie CRC Research Centre, Manchester, UK.en
dc.identifier.journalMammalian Genomeen

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