2.50
Hdl Handle:
http://hdl.handle.net/10541/97505
Title:
Cloning of the murine homolog of the leukemia-associated PML gene.
Authors:
Goddard, A D; Yuan, J Q; Fairbairn, Leslie J; Dexter, T Michael; Borrow, J; Kozak, C; Solomon, E
Abstract:
PML, a Ring-finger protein, participates in the disruption of normal myeloid differentiation when fused to the retinoic acid receptor alpha (RAR alpha) by the translocation between chromosomes (Chrs) 15 and 17 in acute promyelocytic leukemia (APL). As an initial step in the characterization of PML in species other than human, a murine cDNA clone of the PML gene was isolated and sequenced, and the intron/exon organization of the murine locus determined. The predicted amino acid sequence of the mouse PML protein shows 80% similarity to that of its human homolog. However, the mouse and human proteins show greater than 90% similarity in the proposed functional domains of the proteins. Despite its role in the etiology of APL, PML expression is not detectably altered during granulocytic differentiation in a murine in vitro system. Chromosomal localization of the Pml locus by somatic cell hybrids and by linkage analysis indicates that the gene maps to a region of mouse Chr 9 with known linkage homology to the region on human Chr 15q to which PML has been localized.
Affiliation:
Imperial Cancer Research Fund, London, UK.
Citation:
Cloning of the murine homolog of the leukemia-associated PML gene. 1995, 6 (10):732-7 Mamm. Genome
Journal:
Mammalian Genome
Issue Date:
Oct-1995
URI:
http://hdl.handle.net/10541/97505
DOI:
10.1007/BF00354296
PubMed ID:
8563172
Type:
Article
Language:
en
ISSN:
0938-8990
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorGoddard, A Den
dc.contributor.authorYuan, J Qen
dc.contributor.authorFairbairn, Leslie Jen
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorBorrow, Jen
dc.contributor.authorKozak, Cen
dc.contributor.authorSolomon, Een
dc.date.accessioned2010-04-27T16:23:40Z-
dc.date.available2010-04-27T16:23:40Z-
dc.date.issued1995-10-
dc.identifier.citationCloning of the murine homolog of the leukemia-associated PML gene. 1995, 6 (10):732-7 Mamm. Genomeen
dc.identifier.issn0938-8990-
dc.identifier.pmid8563172-
dc.identifier.doi10.1007/BF00354296-
dc.identifier.urihttp://hdl.handle.net/10541/97505-
dc.description.abstractPML, a Ring-finger protein, participates in the disruption of normal myeloid differentiation when fused to the retinoic acid receptor alpha (RAR alpha) by the translocation between chromosomes (Chrs) 15 and 17 in acute promyelocytic leukemia (APL). As an initial step in the characterization of PML in species other than human, a murine cDNA clone of the PML gene was isolated and sequenced, and the intron/exon organization of the murine locus determined. The predicted amino acid sequence of the mouse PML protein shows 80% similarity to that of its human homolog. However, the mouse and human proteins show greater than 90% similarity in the proposed functional domains of the proteins. Despite its role in the etiology of APL, PML expression is not detectably altered during granulocytic differentiation in a murine in vitro system. Chromosomal localization of the Pml locus by somatic cell hybrids and by linkage analysis indicates that the gene maps to a region of mouse Chr 9 with known linkage homology to the region on human Chr 15q to which PML has been localized.en
dc.language.isoenen
dc.subjectCancer Proteinsen
dc.subjectTumour Suppressor Proteinsen
dc.subject.mesh3T3 Cells-
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshCells, Cultured-
dc.subject.meshChromosome Mapping-
dc.subject.meshCloning, Molecular-
dc.subject.meshConserved Sequence-
dc.subject.meshDNA, Complementary-
dc.subject.meshHumans-
dc.subject.meshMice-
dc.subject.meshMice, Inbred BALB C-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshNeoplasm Proteins-
dc.subject.meshNuclear Proteins-
dc.subject.meshSequence Homology, Amino Acid-
dc.subject.meshTranscription Factors-
dc.subject.meshTumor Suppressor Proteins-
dc.titleCloning of the murine homolog of the leukemia-associated PML gene.en
dc.typeArticleen
dc.contributor.departmentImperial Cancer Research Fund, London, UK.en
dc.identifier.journalMammalian Genomeen

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