Early gene signalling-dependent and -independent induction of apoptosis in Ramos human B cells can be inhibited by over-expression of Bcl-2.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97497
Title:
Early gene signalling-dependent and -independent induction of apoptosis in Ramos human B cells can be inhibited by over-expression of Bcl-2.
Authors:
Ning, Z Q; Norton, John D; Johnson, Diane; Murphy, J J
Abstract:
We have previously shown that calcium ionophore-induced apoptosis of Ramos human B cells is preceded by the induced expression of early response genes, implying a requirement for new gene expression in this mode of programmed cell death. We have found in the present studies that inhibitors of macromolecular synthesis, cycloheximide and actinomycin D, are also potent inducers of apoptosis in the same Ramos cell model. These drugs trigger apoptosis through apparently early gene signalling-independent pathways. Although different mechanisms for induction of apoptosis exist in Ramos cells, enforced over-expression of Bcl-2 protects cells from apoptosis induced in response to different agents, demonstrating that Bcl-2 blocks a final common pathway for programmed cell death in the Ramos cell model.
Affiliation:
Division of Life Sciences, King's College London, UK.
Citation:
Early gene signalling-dependent and -independent induction of apoptosis in Ramos human B cells can be inhibited by over-expression of Bcl-2. 1995, 215 (1):23-9 Biochem. Biophys. Res. Commun.
Journal:
Biochemical and Biophysical Research Communications
Issue Date:
4-Oct-1995
URI:
http://hdl.handle.net/10541/97497
DOI:
10.1006/bbrc.1995.2429
PubMed ID:
7575596
Type:
Article
Language:
en
ISSN:
0006-291X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorNing, Z Qen
dc.contributor.authorNorton, John Den
dc.contributor.authorJohnson, Dianeen
dc.contributor.authorMurphy, J Jen
dc.date.accessioned2010-04-27T15:05:33Z-
dc.date.available2010-04-27T15:05:33Z-
dc.date.issued1995-10-04-
dc.identifier.citationEarly gene signalling-dependent and -independent induction of apoptosis in Ramos human B cells can be inhibited by over-expression of Bcl-2. 1995, 215 (1):23-9 Biochem. Biophys. Res. Commun.en
dc.identifier.issn0006-291X-
dc.identifier.pmid7575596-
dc.identifier.doi10.1006/bbrc.1995.2429-
dc.identifier.urihttp://hdl.handle.net/10541/97497-
dc.description.abstractWe have previously shown that calcium ionophore-induced apoptosis of Ramos human B cells is preceded by the induced expression of early response genes, implying a requirement for new gene expression in this mode of programmed cell death. We have found in the present studies that inhibitors of macromolecular synthesis, cycloheximide and actinomycin D, are also potent inducers of apoptosis in the same Ramos cell model. These drugs trigger apoptosis through apparently early gene signalling-independent pathways. Although different mechanisms for induction of apoptosis exist in Ramos cells, enforced over-expression of Bcl-2 protects cells from apoptosis induced in response to different agents, demonstrating that Bcl-2 blocks a final common pathway for programmed cell death in the Ramos cell model.en
dc.language.isoenen
dc.subject.meshApoptosis-
dc.subject.meshB-Lymphocytes-
dc.subject.meshCalcimycin-
dc.subject.meshCell Line-
dc.subject.meshCycloheximide-
dc.subject.meshDactinomycin-
dc.subject.meshGene Expression-
dc.subject.meshHumans-
dc.subject.meshKinetics-
dc.subject.meshProtein Synthesis Inhibitors-
dc.subject.meshProto-Oncogene Proteins-
dc.subject.meshProto-Oncogene Proteins c-bcl-2-
dc.subject.meshSignal Transduction-
dc.subject.meshTransfection-
dc.titleEarly gene signalling-dependent and -independent induction of apoptosis in Ramos human B cells can be inhibited by over-expression of Bcl-2.en
dc.typeArticleen
dc.contributor.departmentDivision of Life Sciences, King's College London, UK.en
dc.identifier.journalBiochemical and Biophysical Research Communicationsen
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