Early gene signalling-dependent and -independent induction of apoptosis in Ramos human B cells can be inhibited by over-expression of Bcl-2.
Affiliation
Division of Life Sciences, King's College London, UK.Issue Date
1995-10-04
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Show full item recordAbstract
We have previously shown that calcium ionophore-induced apoptosis of Ramos human B cells is preceded by the induced expression of early response genes, implying a requirement for new gene expression in this mode of programmed cell death. We have found in the present studies that inhibitors of macromolecular synthesis, cycloheximide and actinomycin D, are also potent inducers of apoptosis in the same Ramos cell model. These drugs trigger apoptosis through apparently early gene signalling-independent pathways. Although different mechanisms for induction of apoptosis exist in Ramos cells, enforced over-expression of Bcl-2 protects cells from apoptosis induced in response to different agents, demonstrating that Bcl-2 blocks a final common pathway for programmed cell death in the Ramos cell model.Citation
Early gene signalling-dependent and -independent induction of apoptosis in Ramos human B cells can be inhibited by over-expression of Bcl-2. 1995, 215 (1):23-9 Biochem. Biophys. Res. Commun.Journal
Biochemical and Biophysical Research CommunicationsDOI
10.1006/bbrc.1995.2429PubMed ID
7575596Type
ArticleLanguage
enISSN
0006-291Xae974a485f413a2113503eed53cd6c53
10.1006/bbrc.1995.2429