Role of interleukin-3 in the regulation of intracellular K+ homeostasis in cultured murine haemopoietic cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97495
Title:
Role of interleukin-3 in the regulation of intracellular K+ homeostasis in cultured murine haemopoietic cells.
Authors:
Laskay, Gabor; Várhelyi, T; Dale, Robert E; Dexter, T Michael
Abstract:
Comparative fluorimetric, flow cytofluorimetric and fluorescence ratiometric determinations of intracellular K+ concentrations in murine haemopoietic cells (FDCP-Mix clone A4) cultured in the presence and absence of the specific growth factor Interleukin-3 were carried out with the fluorescent potassium-binding benzofuran-isophthalate acetoxymethyl ester probe. Cell suspensions kept in the absence of Interleukin-3 for 5 hours exhibited lower fluorescence intensities and smaller fluorescence ratios than their growth-factor-replete counterparts, an effect found to be reversible by readdition of the growth factor. It is concluded that Interleukin-3 deprivation of these cells leads to loss of intracellular K+. It is tentatively suggested that this deprivation-induced K+ loss might be associated with an early event in apoptotic cell death.
Affiliation:
Department of Physics and Instrumentation, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.
Citation:
Role of interleukin-3 in the regulation of intracellular K+ homeostasis in cultured murine haemopoietic cells. 1995, 214 (2):348-53 Biochem. Biophys. Res. Commun.
Journal:
Biochemical and Biophysical Research Communications
Issue Date:
14-Sep-1995
URI:
http://hdl.handle.net/10541/97495
DOI:
10.1006/bbrc.1995.2294
PubMed ID:
7677740
Type:
Article
Language:
en
ISSN:
0006-291X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLaskay, Gaboren
dc.contributor.authorVárhelyi, Ten
dc.contributor.authorDale, Robert Een
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2010-04-27T15:02:29Z-
dc.date.available2010-04-27T15:02:29Z-
dc.date.issued1995-09-14-
dc.identifier.citationRole of interleukin-3 in the regulation of intracellular K+ homeostasis in cultured murine haemopoietic cells. 1995, 214 (2):348-53 Biochem. Biophys. Res. Commun.en
dc.identifier.issn0006-291X-
dc.identifier.pmid7677740-
dc.identifier.doi10.1006/bbrc.1995.2294-
dc.identifier.urihttp://hdl.handle.net/10541/97495-
dc.description.abstractComparative fluorimetric, flow cytofluorimetric and fluorescence ratiometric determinations of intracellular K+ concentrations in murine haemopoietic cells (FDCP-Mix clone A4) cultured in the presence and absence of the specific growth factor Interleukin-3 were carried out with the fluorescent potassium-binding benzofuran-isophthalate acetoxymethyl ester probe. Cell suspensions kept in the absence of Interleukin-3 for 5 hours exhibited lower fluorescence intensities and smaller fluorescence ratios than their growth-factor-replete counterparts, an effect found to be reversible by readdition of the growth factor. It is concluded that Interleukin-3 deprivation of these cells leads to loss of intracellular K+. It is tentatively suggested that this deprivation-induced K+ loss might be associated with an early event in apoptotic cell death.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshBenzofurans-
dc.subject.meshCell Line-
dc.subject.meshCulture Media, Conditioned-
dc.subject.meshEthers, Cyclic-
dc.subject.meshFlow Cytometry-
dc.subject.meshFluorescent Dyes-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHomeostasis-
dc.subject.meshInterleukin-3-
dc.subject.meshMice-
dc.subject.meshPotassium-
dc.subject.meshSpectrometry, Fluorescence-
dc.titleRole of interleukin-3 in the regulation of intracellular K+ homeostasis in cultured murine haemopoietic cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Physics and Instrumentation, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.en
dc.identifier.journalBiochemical and Biophysical Research Communicationsen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.