Cross-resistance studies on two K562 sublines resistant to diaziridinylbenzoquinones.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97489
Title:
Cross-resistance studies on two K562 sublines resistant to diaziridinylbenzoquinones.
Authors:
Ward, Timothy H; Haran, M Sally; Whittaker, Dee; Watson, Amanda J; Howard, Tina D; Butler, John
Abstract:
Two resistant K562 sublines have been developed by treatment with AZQ (2,5-bis(carboethoxyamino)-3,6-diaziridinyl-1,4-benzoquinone) and BZQ (2,5-bis(2-hydroxyethylamino)-3,6-diaziridinyl-1,4-benzoquinone). The ID50 values of for AZQ on K562, the AZQ-resistant sublines (AZQR) and the BZQ-resistant sublines (BZQR) were 0.063, 1.47 and 0.244 microM, respectively. The relative ID50 values for BZQ on the same cell lines were 0.2, 0.67 and 0.83 microM, respectively. Although it is generally believed that these two quinones function by different mechanisms, the two sublines have similar decreased levels of cytochrome P-450 reductase and DT-diaphorase and increased levels of glutathione and superoxide dismutase, compared to the parent cell line. The sublines are also cross-resistant to adriamycin, mitozolamide, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and mitomycin C. This work indicates the potential multifactorial mechanisms by which drug resistance can be induced in cell lines in the absence of conventional 'P'-glycoprotein multidrug resistance.
Affiliation:
CRC Department of Cell Culture, Paterson Institute for Cancer Research, Christie Hospital, Manchester, U.K.
Citation:
Cross-resistance studies on two K562 sublines resistant to diaziridinylbenzoquinones. 1995, 50 (4):459-64 Biochem. Pharmacol.
Journal:
Biochemical Pharmacology
Issue Date:
8-Aug-1995
URI:
http://hdl.handle.net/10541/97489
DOI:
10.1016/0006-2952(95)00155-S
PubMed ID:
7646550
Type:
Article
Language:
en
ISSN:
0006-2952
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWard, Timothy Hen
dc.contributor.authorHaran, M Sallyen
dc.contributor.authorWhittaker, Deeen
dc.contributor.authorWatson, Amanda Jen
dc.contributor.authorHoward, Tina Den
dc.contributor.authorButler, Johnen
dc.date.accessioned2010-04-27T14:50:42Z-
dc.date.available2010-04-27T14:50:42Z-
dc.date.issued1995-08-08-
dc.identifier.citationCross-resistance studies on two K562 sublines resistant to diaziridinylbenzoquinones. 1995, 50 (4):459-64 Biochem. Pharmacol.en
dc.identifier.issn0006-2952-
dc.identifier.pmid7646550-
dc.identifier.doi10.1016/0006-2952(95)00155-S-
dc.identifier.urihttp://hdl.handle.net/10541/97489-
dc.description.abstractTwo resistant K562 sublines have been developed by treatment with AZQ (2,5-bis(carboethoxyamino)-3,6-diaziridinyl-1,4-benzoquinone) and BZQ (2,5-bis(2-hydroxyethylamino)-3,6-diaziridinyl-1,4-benzoquinone). The ID50 values of for AZQ on K562, the AZQ-resistant sublines (AZQR) and the BZQ-resistant sublines (BZQR) were 0.063, 1.47 and 0.244 microM, respectively. The relative ID50 values for BZQ on the same cell lines were 0.2, 0.67 and 0.83 microM, respectively. Although it is generally believed that these two quinones function by different mechanisms, the two sublines have similar decreased levels of cytochrome P-450 reductase and DT-diaphorase and increased levels of glutathione and superoxide dismutase, compared to the parent cell line. The sublines are also cross-resistant to adriamycin, mitozolamide, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and mitomycin C. This work indicates the potential multifactorial mechanisms by which drug resistance can be induced in cell lines in the absence of conventional 'P'-glycoprotein multidrug resistance.en
dc.language.isoenen
dc.subject.meshAziridines-
dc.subject.meshBenzoquinones-
dc.subject.meshCell Division-
dc.subject.meshCell Line-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshDrug Resistance-
dc.subject.meshGlutathione-
dc.subject.meshHumans-
dc.subject.meshNAD(P)H Dehydrogenase (Quinone)-
dc.subject.meshNADPH-Ferrihemoprotein Reductase-
dc.subject.meshOxidoreductases-
dc.subject.meshP-Glycoprotein-
dc.subject.meshSuperoxide Dismutase-
dc.titleCross-resistance studies on two K562 sublines resistant to diaziridinylbenzoquinones.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Cell Culture, Paterson Institute for Cancer Research, Christie Hospital, Manchester, U.K.en
dc.identifier.journalBiochemical Pharmacologyen

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