Cross-resistance studies on two K562 sublines resistant to diaziridinylbenzoquinones.
Affiliation
CRC Department of Cell Culture, Paterson Institute for Cancer Research, Christie Hospital, Manchester, U.K.Issue Date
1995-08-08
Metadata
Show full item recordAbstract
Two resistant K562 sublines have been developed by treatment with AZQ (2,5-bis(carboethoxyamino)-3,6-diaziridinyl-1,4-benzoquinone) and BZQ (2,5-bis(2-hydroxyethylamino)-3,6-diaziridinyl-1,4-benzoquinone). The ID50 values of for AZQ on K562, the AZQ-resistant sublines (AZQR) and the BZQ-resistant sublines (BZQR) were 0.063, 1.47 and 0.244 microM, respectively. The relative ID50 values for BZQ on the same cell lines were 0.2, 0.67 and 0.83 microM, respectively. Although it is generally believed that these two quinones function by different mechanisms, the two sublines have similar decreased levels of cytochrome P-450 reductase and DT-diaphorase and increased levels of glutathione and superoxide dismutase, compared to the parent cell line. The sublines are also cross-resistant to adriamycin, mitozolamide, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and mitomycin C. This work indicates the potential multifactorial mechanisms by which drug resistance can be induced in cell lines in the absence of conventional 'P'-glycoprotein multidrug resistance.Citation
Cross-resistance studies on two K562 sublines resistant to diaziridinylbenzoquinones. 1995, 50 (4):459-64 Biochem. Pharmacol.Journal
Biochemical PharmacologyDOI
10.1016/0006-2952(95)00155-SPubMed ID
7646550Type
ArticleLanguage
enISSN
0006-2952ae974a485f413a2113503eed53cd6c53
10.1016/0006-2952(95)00155-S
Scopus Count
Collections
Related articles
- Comet assay studies on the activation of two diaziridinylbenzoquinones in K562 cells.
- Authors: Ward TH, Butler J, Shahbakhti H, Richards JT
- Issue date: 1997 Apr 25
- The lack of correlation between toxicity and free radical formation of two diaziridinyl benzoquinones.
- Authors: Lea JS, Garner HJ, Butler J, Hoey BM, Ward TH
- Issue date: 1988 May 15
- Relationship between DT-diaphorase-mediated metabolism of a series of aziridinylbenzoquinones and DNA damage and cytotoxicity.
- Authors: Gibson NW, Hartley JA, Butler J, Siegel D, Ross D
- Issue date: 1992 Sep
- Metabolism of diaziquone by NAD(P)H:(quinone acceptor) oxidoreductase (DT-diaphorase): role in diaziquone-induced DNA damage and cytotoxicity in human colon carcinoma cells.
- Authors: Siegel D, Gibson NW, Preusch PC, Ross D
- Issue date: 1990 Nov 15
- In vitro multidrug resistance of P388 murine leukemia selected for resistance to diaziquone.
- Authors: Gutierrez PL, Wilder PJ, Biswal N
- Issue date: 1989