Effects of interleukin-6 on mobilization of primitive haemopoietic cells into the circulation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97483
Title:
Effects of interleukin-6 on mobilization of primitive haemopoietic cells into the circulation.
Authors:
Pettengell, Ruth; Luft, Thomas; De Wynter, Erika A; Coutinho, Lucia H; Young, Rosemary; Fitzsimmons, Lesley; Scarffe, J Howard; Testa, Nydia G
Abstract:
Twenty-seven patients with advanced adenocarcinoma were studied. Groups of three patients received interleukin-6 (IL-6) in doses ranging from 0.5 to 20 micrograms/kg by daily subcutaneous injection on days 1-7 and 22-49. Four patients received IL-6 2.5 micrograms/kg/d with GM-CSF 5 micrograms/kg/d and three patients received IL-6 2.5 micrograms/kg/d with IL-3 5 micrograms/kg/d. Circulating platelet numbers increased 1.65-fold during IL-6 treatment, in a dose-dependent fashion (P = 0.01). This increase is inferior to that expected from laboratory studies. No significant change in total WBC was seen after IL-6 alone. After treatment with IL-6, significant increases in numbers of circulating mononuclear cells (2.2-fold, P = 0.006) and GM-CFC numbers (3.2-fold, P = 0.01) were seen, but there were no changes in circulating megakaryocyte-CFC numbers. In contrast, after treatment with IL-6 and GM-CSF, larger increases in both circulating GM-CFC (20-fold, P = 0.04) and megakaryocyte-CFC numbers (18-fold, P = 0.03) were seen. Increases in blood progenitors after treatment with IL-6 and IL-3 did not achieve statistical significance. The ability of peripheral blood mononuclear cells to generate and sustain long-term haemopoiesis in vitro was similar in IL-6-treated patients to that in untreated control subjects. No significant changes in the incidence of bone marrow progenitors or their cycling status (assessed by thymidine suicide) were seen. These data suggest that IL-6 alone will not be clinically useful to mobilize blood progenitor cells in cancer patients.
Affiliation:
CRC Department of Medical Oncology, Christie Hospital, Manchester.
Citation:
Effects of interleukin-6 on mobilization of primitive haemopoietic cells into the circulation. 1995, 89 (2):237-42 Br. J. Haematol.
Journal:
British Journal of Haematology
Issue Date:
Feb-1995
URI:
http://hdl.handle.net/10541/97483
DOI:
10.1111/j.1365-2141.1995.tb03295.x
PubMed ID:
7873372
Type:
Article
Language:
en
ISSN:
0007-1048
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPettengell, Ruthen
dc.contributor.authorLuft, Thomasen
dc.contributor.authorDe Wynter, Erika Aen
dc.contributor.authorCoutinho, Lucia Hen
dc.contributor.authorYoung, Rosemaryen
dc.contributor.authorFitzsimmons, Lesleyen
dc.contributor.authorScarffe, J Howarden
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2010-04-27T13:41:22Z-
dc.date.available2010-04-27T13:41:22Z-
dc.date.issued1995-02-
dc.identifier.citationEffects of interleukin-6 on mobilization of primitive haemopoietic cells into the circulation. 1995, 89 (2):237-42 Br. J. Haematol.en
dc.identifier.issn0007-1048-
dc.identifier.pmid7873372-
dc.identifier.doi10.1111/j.1365-2141.1995.tb03295.x-
dc.identifier.urihttp://hdl.handle.net/10541/97483-
dc.description.abstractTwenty-seven patients with advanced adenocarcinoma were studied. Groups of three patients received interleukin-6 (IL-6) in doses ranging from 0.5 to 20 micrograms/kg by daily subcutaneous injection on days 1-7 and 22-49. Four patients received IL-6 2.5 micrograms/kg/d with GM-CSF 5 micrograms/kg/d and three patients received IL-6 2.5 micrograms/kg/d with IL-3 5 micrograms/kg/d. Circulating platelet numbers increased 1.65-fold during IL-6 treatment, in a dose-dependent fashion (P = 0.01). This increase is inferior to that expected from laboratory studies. No significant change in total WBC was seen after IL-6 alone. After treatment with IL-6, significant increases in numbers of circulating mononuclear cells (2.2-fold, P = 0.006) and GM-CFC numbers (3.2-fold, P = 0.01) were seen, but there were no changes in circulating megakaryocyte-CFC numbers. In contrast, after treatment with IL-6 and GM-CSF, larger increases in both circulating GM-CFC (20-fold, P = 0.04) and megakaryocyte-CFC numbers (18-fold, P = 0.03) were seen. Increases in blood progenitors after treatment with IL-6 and IL-3 did not achieve statistical significance. The ability of peripheral blood mononuclear cells to generate and sustain long-term haemopoiesis in vitro was similar in IL-6-treated patients to that in untreated control subjects. No significant changes in the incidence of bone marrow progenitors or their cycling status (assessed by thymidine suicide) were seen. These data suggest that IL-6 alone will not be clinically useful to mobilize blood progenitor cells in cancer patients.en
dc.language.isoenen
dc.subjectColorectal Canceren
dc.subjectHaematopoietic Stem Cellsen
dc.subjectUnknown Primary Canceren
dc.subjectPancreatic Canceren
dc.subjectTumour Stem Cell Assayen
dc.subject.meshAdenocarcinoma-
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshBlood Platelets-
dc.subject.meshColorectal Neoplasms-
dc.subject.meshDrug Combinations-
dc.subject.meshFemale-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshInterleukin-3-
dc.subject.meshInterleukin-6-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshNeoplasms, Unknown Primary-
dc.subject.meshPancreatic Neoplasms-
dc.subject.meshTumor Stem Cell Assay-
dc.titleEffects of interleukin-6 on mobilization of primitive haemopoietic cells into the circulation.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Medical Oncology, Christie Hospital, Manchester.en
dc.identifier.journalBritish Journal of Haematologyen

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