MIP-1 alpha increases the self-renewal capacity of the hemopoietic spleen-colony-forming cells following hydroxyurea treatment in vivo.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97480
Title:
MIP-1 alpha increases the self-renewal capacity of the hemopoietic spleen-colony-forming cells following hydroxyurea treatment in vivo.
Authors:
Lord, Brian I
Abstract:
In this report, the effect of macrophage inflammatory protein (MIP-1alpha) on the self-renewal, in vivo, of haemopoietic spleen colony-forming units (CFU-S) following cytotoxic damage, has been investigated. CFU-S recovery following injection of hydroxyurea, HU (1 g/kg at 0 and 7 hers) with or without MIP-1alpha intervention was measured over the following 5 days. In addition to the initial protection conferred by MIP-1alpha, the CFU-S population recovered about 1.7 times faster than in the unprotected controls. Direct measurement of the self-renewal capacity of the CFU-S population was made at 2 days after HU + MIP-1alpha treatment by measuring the number of CFU-S/spleen colony in a secondary transplant assay. CFU-S following HU treatment alone generated 60 CFU-S/colony. Additional MIP-1alpha treatment increased this to 90 CFU-S/colony. It is concluded that MIP-1alpha modifies the generation age structure of a regenerating CFU-S population such that recovery is initiated from the more primitive cells of the population's age spectrum and that this observation should extend the range of cytotoxic agents from which MIP-1alpha can give protection.
Affiliation:
CRC Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
MIP-1 alpha increases the self-renewal capacity of the hemopoietic spleen-colony-forming cells following hydroxyurea treatment in vivo. 1995, 12 (2):145-9 Growth Factors
Journal:
Growth Factors
Issue Date:
1995
URI:
http://hdl.handle.net/10541/97480
DOI:
10.3109/08977199509028960
PubMed ID:
8679248
Type:
Article
Language:
en
ISSN:
0897-7194
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLord, Brian Ien
dc.date.accessioned2010-04-27T13:17:09Z-
dc.date.available2010-04-27T13:17:09Z-
dc.date.issued1995-
dc.identifier.citationMIP-1 alpha increases the self-renewal capacity of the hemopoietic spleen-colony-forming cells following hydroxyurea treatment in vivo. 1995, 12 (2):145-9 Growth Factorsen
dc.identifier.issn0897-7194-
dc.identifier.pmid8679248-
dc.identifier.doi10.3109/08977199509028960-
dc.identifier.urihttp://hdl.handle.net/10541/97480-
dc.description.abstractIn this report, the effect of macrophage inflammatory protein (MIP-1alpha) on the self-renewal, in vivo, of haemopoietic spleen colony-forming units (CFU-S) following cytotoxic damage, has been investigated. CFU-S recovery following injection of hydroxyurea, HU (1 g/kg at 0 and 7 hers) with or without MIP-1alpha intervention was measured over the following 5 days. In addition to the initial protection conferred by MIP-1alpha, the CFU-S population recovered about 1.7 times faster than in the unprotected controls. Direct measurement of the self-renewal capacity of the CFU-S population was made at 2 days after HU + MIP-1alpha treatment by measuring the number of CFU-S/spleen colony in a secondary transplant assay. CFU-S following HU treatment alone generated 60 CFU-S/colony. Additional MIP-1alpha treatment increased this to 90 CFU-S/colony. It is concluded that MIP-1alpha modifies the generation age structure of a regenerating CFU-S population such that recovery is initiated from the more primitive cells of the population's age spectrum and that this observation should extend the range of cytotoxic agents from which MIP-1alpha can give protection.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshChemokine CCL3-
dc.subject.meshChemokine CCL4-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHydroxyurea-
dc.subject.meshMacrophage Inflammatory Proteins-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshMice, Inbred DBA-
dc.subject.meshMonokines-
dc.subject.meshSpleen-
dc.titleMIP-1 alpha increases the self-renewal capacity of the hemopoietic spleen-colony-forming cells following hydroxyurea treatment in vivo.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalGrowth Factorsen

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