2.50
Hdl Handle:
http://hdl.handle.net/10541/97476
Title:
Loss of heterozygosity on the X chromosome in human breast cancer.
Authors:
Loupart, M L; Adams, S; Armour, J A; Walker, R; Brammar, W; Varley, Jennifer
Abstract:
The analysis of loss of heterozygosity (LOH) in tumours can be a powerful tool for mapping the sites of tumour suppressor genes in the human genome. A panel of breast cancer patients was assembled as pairs of tumour and lymphocyte DNA samples and LOH studies carried out by Southern hybridisation with polymorphic loci mapping to the X chromosome with appropriate controls. Deletion mapping revealed a high frequency of small regionalised deletions, defining at least three independent regions, one of which is particularly well mapped to a 500 kb stretch of DNA in the distal portion of the pseudoautosomal region of Xp. A second region has been identified within the pseudoautosomal region close to the pseudoautosomal boundary, and there is a third discrete site of loss on distal Xq. Perturbations of sequences at these regions represent independent events in a number of patients. This study represents the first detailed analysis of LOH on the X chromosome in human breast tumours, the results of which indicate that at least three regions of this chromosome are involved in the disease.
Affiliation:
ICI/University Joint Laboratory, University of Leicester, United Kingdom.
Citation:
Loss of heterozygosity on the X chromosome in human breast cancer. 1995, 13 (4):229-38 Genes Chromosomes Cancer
Journal:
Genes, Chromosomes & Cancer
Issue Date:
Aug-1995
URI:
http://hdl.handle.net/10541/97476
DOI:
10.1002/gcc.2870130402
PubMed ID:
7547630
Type:
Article
Language:
en
ISSN:
1045-2257
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLoupart, M Len
dc.contributor.authorAdams, Sen
dc.contributor.authorArmour, J Aen
dc.contributor.authorWalker, Ren
dc.contributor.authorBrammar, Wen
dc.contributor.authorVarley, Jenniferen
dc.date.accessioned2010-04-27T12:43:10Z-
dc.date.available2010-04-27T12:43:10Z-
dc.date.issued1995-08-
dc.identifier.citationLoss of heterozygosity on the X chromosome in human breast cancer. 1995, 13 (4):229-38 Genes Chromosomes Canceren
dc.identifier.issn1045-2257-
dc.identifier.pmid7547630-
dc.identifier.doi10.1002/gcc.2870130402-
dc.identifier.urihttp://hdl.handle.net/10541/97476-
dc.description.abstractThe analysis of loss of heterozygosity (LOH) in tumours can be a powerful tool for mapping the sites of tumour suppressor genes in the human genome. A panel of breast cancer patients was assembled as pairs of tumour and lymphocyte DNA samples and LOH studies carried out by Southern hybridisation with polymorphic loci mapping to the X chromosome with appropriate controls. Deletion mapping revealed a high frequency of small regionalised deletions, defining at least three independent regions, one of which is particularly well mapped to a 500 kb stretch of DNA in the distal portion of the pseudoautosomal region of Xp. A second region has been identified within the pseudoautosomal region close to the pseudoautosomal boundary, and there is a third discrete site of loss on distal Xq. Perturbations of sequences at these regions represent independent events in a number of patients. This study represents the first detailed analysis of LOH on the X chromosome in human breast tumours, the results of which indicate that at least three regions of this chromosome are involved in the disease.en
dc.language.isoenen
dc.subjectBreast Canceren
dc.subject.meshAlleles-
dc.subject.meshBreast Neoplasms-
dc.subject.meshChromosome Deletion-
dc.subject.meshChromosome Mapping-
dc.subject.meshFemale-
dc.subject.meshGenetic Markers-
dc.subject.meshHeterozygote-
dc.subject.meshHumans-
dc.subject.meshPolymorphism, Genetic-
dc.subject.meshX Chromosome-
dc.titleLoss of heterozygosity on the X chromosome in human breast cancer.en
dc.typeArticleen
dc.contributor.departmentICI/University Joint Laboratory, University of Leicester, United Kingdom.en
dc.identifier.journalGenes, Chromosomes & Canceren

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