Naloxone behaves as opioid agonist/antagonist in clonal cultures of mouse bone marrow cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97464
Title:
Naloxone behaves as opioid agonist/antagonist in clonal cultures of mouse bone marrow cells.
Authors:
Krizanac-Bengez, L; Boranić, M; Testa, Nydia G
Abstract:
The opioid peptide methionine (Met)-enkephalin and the opioid-receptor blocking agent naloxone were added to unseparated or to progenitor-enriched cell suspensions of mouse bone marrow before assay in clonal cultures. Bone marrow samples harvested at 18:00 hours produced more granulocyte-macrophage (GM) colonies than the 06:00 hour samples, and were more sensitive to the proliferation inhibition by both agents. Additive inhibitory effects of naloxone with the enkephalin were occasionally seen. Thus, in this experimental system, naloxone could behave as an opioid agonist. However, there were examples of naloxone diminishing (blocking) the suppressive effect of the enkephalin, as a true opioid antagonist. Significant naloxone/enkephalin interactions occurred in opioid-sensitive (18:00 h) samples of unseparated bone marrow. The interactions were virtually absent in progenitor cell-enriched populations, indicating a significant role of accessory cells in opioidergic regulation of hematopoietic progenitors.
Affiliation:
Ruder Bosković Institute, Zagreb, Croatia.
Citation:
Naloxone behaves as opioid agonist/antagonist in clonal cultures of mouse bone marrow cells. 1995, 49 (1):27-31 Biomed. Pharmacother.
Journal:
Biomedicine & Pharmacotherapy
Issue Date:
1995
URI:
http://hdl.handle.net/10541/97464
DOI:
10.1016/0753-3322(96)82574-1
PubMed ID:
7749076
Type:
Article
Language:
en
ISSN:
0753-3322
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorKrizanac-Bengez, Len
dc.contributor.authorBoranić, Men
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2010-04-27T14:36:26Z-
dc.date.available2010-04-27T14:36:26Z-
dc.date.issued1995-
dc.identifier.citationNaloxone behaves as opioid agonist/antagonist in clonal cultures of mouse bone marrow cells. 1995, 49 (1):27-31 Biomed. Pharmacother.en
dc.identifier.issn0753-3322-
dc.identifier.pmid7749076-
dc.identifier.doi10.1016/0753-3322(96)82574-1-
dc.identifier.urihttp://hdl.handle.net/10541/97464-
dc.description.abstractThe opioid peptide methionine (Met)-enkephalin and the opioid-receptor blocking agent naloxone were added to unseparated or to progenitor-enriched cell suspensions of mouse bone marrow before assay in clonal cultures. Bone marrow samples harvested at 18:00 hours produced more granulocyte-macrophage (GM) colonies than the 06:00 hour samples, and were more sensitive to the proliferation inhibition by both agents. Additive inhibitory effects of naloxone with the enkephalin were occasionally seen. Thus, in this experimental system, naloxone could behave as an opioid agonist. However, there were examples of naloxone diminishing (blocking) the suppressive effect of the enkephalin, as a true opioid antagonist. Significant naloxone/enkephalin interactions occurred in opioid-sensitive (18:00 h) samples of unseparated bone marrow. The interactions were virtually absent in progenitor cell-enriched populations, indicating a significant role of accessory cells in opioidergic regulation of hematopoietic progenitors.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBone Marrow Cells-
dc.subject.meshCell Division-
dc.subject.meshCells, Cultured-
dc.subject.meshClone Cells-
dc.subject.meshDrug Therapy, Combination-
dc.subject.meshEnkephalin, Methionine-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshMice, Inbred DBA-
dc.subject.meshNaloxone-
dc.subject.meshStem Cells-
dc.titleNaloxone behaves as opioid agonist/antagonist in clonal cultures of mouse bone marrow cells.en
dc.typeArticleen
dc.contributor.departmentRuder Bosković Institute, Zagreb, Croatia.en
dc.identifier.journalBiomedicine & Pharmacotherapyen

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