2.50
Hdl Handle:
http://hdl.handle.net/10541/97457
Title:
Proximal 6q, a region showing allele loss in primary breast cancer.
Authors:
Orphanos, Vassilis; McGown, Gail; Hey, Yvonne; Boyle, John M; Santibanez-Koref, Mauro F
Abstract:
To define regions of deletion of chromosome 6q in breast cancer, we scored 18 (CA)n microsatellites for allelic imbalance (AI) in 42 paired blood/tumour samples. Heterozygosity frequencies of the markers in the sample population ranged from 31% to 92% (mean 68%). Two regions of the chromosome arm showed AI values greater than the background range of 10-22% (mean 17%) of informative cases that was observed with five markers spanning 6q21-q25.2. Firstly, seven markers gave AI values that averaged 35% in a region flanked by D6S313 (AI = 10%) at 6q13 and D6S283 (AI = 17%) at 6q16.3-21. The second region showed marginally increased AI at 6q25.2-q27 and included D6S193, previously shown to be close to a tumour-suppressor gene involved in ovarian carcinoma. Since AI of 6q in breast cancer was shown previously to be due predominantly to loss of heterozygosity, our results suggest the presence of at least two tumour-suppressor genes on 6q that are involved in breast cancer. The proximal region has not been recognised in breast cancer before and is involved in a higher frequency of tumours than the distal region.
Affiliation:
Cancer Research Campaign Department of Cancer Genetics, Christie CRC Research Centre, Manchester, UK.
Citation:
Proximal 6q, a region showing allele loss in primary breast cancer. 1995, 71 (2):290-3 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Feb-1995
URI:
http://hdl.handle.net/10541/97457
PubMed ID:
7841042
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorOrphanos, Vassilisen
dc.contributor.authorMcGown, Gailen
dc.contributor.authorHey, Yvonneen
dc.contributor.authorBoyle, John Men
dc.contributor.authorSantibanez-Koref, Mauro Fen
dc.date.accessioned2010-04-27T13:23:59Z-
dc.date.available2010-04-27T13:23:59Z-
dc.date.issued1995-02-
dc.identifier.citationProximal 6q, a region showing allele loss in primary breast cancer. 1995, 71 (2):290-3 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid7841042-
dc.identifier.urihttp://hdl.handle.net/10541/97457-
dc.description.abstractTo define regions of deletion of chromosome 6q in breast cancer, we scored 18 (CA)n microsatellites for allelic imbalance (AI) in 42 paired blood/tumour samples. Heterozygosity frequencies of the markers in the sample population ranged from 31% to 92% (mean 68%). Two regions of the chromosome arm showed AI values greater than the background range of 10-22% (mean 17%) of informative cases that was observed with five markers spanning 6q21-q25.2. Firstly, seven markers gave AI values that averaged 35% in a region flanked by D6S313 (AI = 10%) at 6q13 and D6S283 (AI = 17%) at 6q16.3-21. The second region showed marginally increased AI at 6q25.2-q27 and included D6S193, previously shown to be close to a tumour-suppressor gene involved in ovarian carcinoma. Since AI of 6q in breast cancer was shown previously to be due predominantly to loss of heterozygosity, our results suggest the presence of at least two tumour-suppressor genes on 6q that are involved in breast cancer. The proximal region has not been recognised in breast cancer before and is involved in a higher frequency of tumours than the distal region.en
dc.language.isoenen
dc.subjectBreast Canceren
dc.subjectCancer DNAen
dc.subjectTumour Suppressor Genesen
dc.subject.meshAlleles-
dc.subject.meshBase Sequence-
dc.subject.meshBreast Neoplasms-
dc.subject.meshCarcinoma, Ductal, Breast-
dc.subject.meshChromosome Mapping-
dc.subject.meshChromosomes, Human, Pair 6-
dc.subject.meshDNA Mutational Analysis-
dc.subject.meshDNA, Neoplasm-
dc.subject.meshDNA, Satellite-
dc.subject.meshGene Deletion-
dc.subject.meshGenes, Tumor Suppressor-
dc.subject.meshHumans-
dc.subject.meshMolecular Sequence Data-
dc.titleProximal 6q, a region showing allele loss in primary breast cancer.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Cancer Genetics, Christie CRC Research Centre, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

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