Irradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97133
Title:
Irradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans.
Authors:
Pye, David A; Kumar, Shant; Wang, Ji Min; Hunter, Robin D
Abstract:
The effect of X-irradiation on the synthesis of heparan sulphate (HS) and chondroitin/dermatan sulphate (CS/DS) by bovine aortic endothelial cells (BAEC), was studied by measuring the incorporation of [35S]sulphate and [3H]glucosamine into newly synthesized glycosaminoglycan (GAG) chains. Medium extracts from irradiated cultures (5Gy) were found to contain approx. 130% more HS and 200% more CS/DS than unirradiated controls. Smaller increases were observed in cellular extracts, irradiated cultures (5Gy) containing approx. 60% more HS and 100% more CS/DS than unirradiated controls. Structural studies showed no significant changes occurred upon irradiation in either the amounts or distribution of N- and O-sulphate groups in the HS molecule. Values for N-sulphation of 41.1% control and 41.5% irradiated (5Gy) were obtained, the corresponding values for O-sulphation being 19.9% control and 20.2% irradiated. Isotope incorporation data indicated that sulphation of CS/DS may decrease after irradiation, however, analysis of chondroitin ABC lyase derived disaccharides showed no changes in the proportion of non-sulphated and O-sulphated disaccharides. The present study indicates that X-irradiation stimulates the synthesis and secretion of HS and CS/DS proteoglycans (PGs) by BAEC. This could be relevant to many features which are found to be indicative of radiation-induced damage.
Affiliation:
Department of Clinical Research, Christie Hospital, Manchester, UK.
Citation:
Irradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans. 1994, 1220 (3):266-76 Biochim. Biophys. Acta
Journal:
Biochimica et Biophysica Acta
Issue Date:
17-Feb-1994
URI:
http://hdl.handle.net/10541/97133
PubMed ID:
8305499
Type:
Article
Language:
en
ISSN:
0006-3002
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorPye, David Aen
dc.contributor.authorKumar, Shanten
dc.contributor.authorWang, Ji Minen
dc.contributor.authorHunter, Robin Den
dc.date.accessioned2010-04-22T09:27:33Z-
dc.date.available2010-04-22T09:27:33Z-
dc.date.issued1994-02-17-
dc.identifier.citationIrradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans. 1994, 1220 (3):266-76 Biochim. Biophys. Actaen
dc.identifier.issn0006-3002-
dc.identifier.pmid8305499-
dc.identifier.urihttp://hdl.handle.net/10541/97133-
dc.description.abstractThe effect of X-irradiation on the synthesis of heparan sulphate (HS) and chondroitin/dermatan sulphate (CS/DS) by bovine aortic endothelial cells (BAEC), was studied by measuring the incorporation of [35S]sulphate and [3H]glucosamine into newly synthesized glycosaminoglycan (GAG) chains. Medium extracts from irradiated cultures (5Gy) were found to contain approx. 130% more HS and 200% more CS/DS than unirradiated controls. Smaller increases were observed in cellular extracts, irradiated cultures (5Gy) containing approx. 60% more HS and 100% more CS/DS than unirradiated controls. Structural studies showed no significant changes occurred upon irradiation in either the amounts or distribution of N- and O-sulphate groups in the HS molecule. Values for N-sulphation of 41.1% control and 41.5% irradiated (5Gy) were obtained, the corresponding values for O-sulphation being 19.9% control and 20.2% irradiated. Isotope incorporation data indicated that sulphation of CS/DS may decrease after irradiation, however, analysis of chondroitin ABC lyase derived disaccharides showed no changes in the proportion of non-sulphated and O-sulphated disaccharides. The present study indicates that X-irradiation stimulates the synthesis and secretion of HS and CS/DS proteoglycans (PGs) by BAEC. This could be relevant to many features which are found to be indicative of radiation-induced damage.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshAorta-
dc.subject.meshCattle-
dc.subject.meshCells, Cultured-
dc.subject.meshChromatography, Gel-
dc.subject.meshChromatography, Ion Exchange-
dc.subject.meshDose-Response Relationship, Radiation-
dc.subject.meshElectrophoresis, Polyacrylamide Gel-
dc.subject.meshEndothelium, Vascular-
dc.subject.meshGlucosamine-
dc.subject.meshGlycosaminoglycans-
dc.subject.meshMolecular Weight-
dc.subject.meshScintillation Counting-
dc.subject.meshSulfates-
dc.subject.meshSulfur Radioisotopes-
dc.subject.meshTritium-
dc.subject.meshX-Rays-
dc.titleIrradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans.en
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalBiochimica et Biophysica Actaen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.