Differences in MHC and TAP-1 expression in cervical cancer lymph node metastases as compared with the primary tumours.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97078
Title:
Differences in MHC and TAP-1 expression in cervical cancer lymph node metastases as compared with the primary tumours.
Authors:
Cromme, F V; Walboomers, J M; Gallee, M P; Stern, Peter L; Kenemans, P; Helmerhorst, T J; Stukart, M J; Meijer, C J
Abstract:
In previous studies we have shown down-regulation of class I major histocompatibility complex (MHC) expression in a significant proportion of primary cervical carcinomas, which was found to be strongly correlated with loss of expression of the transporter associated with antigen presentation (TAP). By contrast, class II MHC expression was frequently up-regulated on neoplastic keratinocytes in these malignancies. In order to investigate whether these changes are associated with biological behaviour of the tumours, 20 cervical carcinomas were analyzed for MHC (HLA-A, HLA-B/C, HLA-DR) and TAP-1 expression in the primary tumours and in lymph node metastases by immunohistochemistry. The results showed a significant increase in the prevalence of HLA-A and HLA-B/C down-regulation in metastasised neoplastic cells as compared with the primary tumour (P = 0.01). In all cases this was accompanied by loss of TAP-1 expression. Up-regulated HLA-DR expression was found exclusively in primary tumours and was absent in the corresponding metastases (P = 0.002). These data are consistent with the hypothesis that loss of TAP-1 and the consequent down-regulation of class I MHC expression provides a selective advantage for neoplastic cervical cells during metastasis. Furthermore, the lack of class II MHC expression in metastasised cells either reflects a different local lymphokine production or indicates that these cells may have escaped CD4+ cytotoxic T-lymphocyte (CTL)-mediated killing.
Affiliation:
Institute for Pathology, Free University Hospital, Amsterdam, The Netherlands.
Citation:
Differences in MHC and TAP-1 expression in cervical cancer lymph node metastases as compared with the primary tumours. 1994, 69 (6):1176-81 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Jun-1994
URI:
http://hdl.handle.net/10541/97078
PubMed ID:
8198988
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorCromme, F Ven
dc.contributor.authorWalboomers, J Men
dc.contributor.authorGallee, M Pen
dc.contributor.authorStern, Peter Len
dc.contributor.authorKenemans, Pen
dc.contributor.authorHelmerhorst, T Jen
dc.contributor.authorStukart, M Jen
dc.contributor.authorMeijer, C Jen
dc.date.accessioned2010-04-21T13:54:32Z-
dc.date.available2010-04-21T13:54:32Z-
dc.date.issued1994-06-
dc.identifier.citationDifferences in MHC and TAP-1 expression in cervical cancer lymph node metastases as compared with the primary tumours. 1994, 69 (6):1176-81 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid8198988-
dc.identifier.urihttp://hdl.handle.net/10541/97078-
dc.description.abstractIn previous studies we have shown down-regulation of class I major histocompatibility complex (MHC) expression in a significant proportion of primary cervical carcinomas, which was found to be strongly correlated with loss of expression of the transporter associated with antigen presentation (TAP). By contrast, class II MHC expression was frequently up-regulated on neoplastic keratinocytes in these malignancies. In order to investigate whether these changes are associated with biological behaviour of the tumours, 20 cervical carcinomas were analyzed for MHC (HLA-A, HLA-B/C, HLA-DR) and TAP-1 expression in the primary tumours and in lymph node metastases by immunohistochemistry. The results showed a significant increase in the prevalence of HLA-A and HLA-B/C down-regulation in metastasised neoplastic cells as compared with the primary tumour (P = 0.01). In all cases this was accompanied by loss of TAP-1 expression. Up-regulated HLA-DR expression was found exclusively in primary tumours and was absent in the corresponding metastases (P = 0.002). These data are consistent with the hypothesis that loss of TAP-1 and the consequent down-regulation of class I MHC expression provides a selective advantage for neoplastic cervical cells during metastasis. Furthermore, the lack of class II MHC expression in metastasised cells either reflects a different local lymphokine production or indicates that these cells may have escaped CD4+ cytotoxic T-lymphocyte (CTL)-mediated killing.en
dc.language.isoenen
dc.subjectCancer Stagingen
dc.subjectUterine Cervical Canceren
dc.subject.meshATP-Binding Cassette Transporters-
dc.subject.meshCarrier Proteins-
dc.subject.meshFemale-
dc.subject.meshHLA-A Antigens-
dc.subject.meshHLA-B Antigens-
dc.subject.meshHLA-C Antigens-
dc.subject.meshHLA-DR Antigens-
dc.subject.meshHistocompatibility Antigens Class I-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshLymph Node Excision-
dc.subject.meshLymph Nodes-
dc.subject.meshLymphatic Metastasis-
dc.subject.meshMajor Histocompatibility Complex-
dc.subject.meshNeoplasm Staging-
dc.subject.meshUterine Cervical Neoplasms-
dc.titleDifferences in MHC and TAP-1 expression in cervical cancer lymph node metastases as compared with the primary tumours.en
dc.typeArticleen
dc.contributor.departmentInstitute for Pathology, Free University Hospital, Amsterdam, The Netherlands.en
dc.identifier.journalBritish Journal of Canceren

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