Growth of fibroblasts as a potential confounding factor in soft agar clonogenic assays for tumour cell radiosensitivity.

2.50
Hdl Handle:
http://hdl.handle.net/10541/97065
Title:
Growth of fibroblasts as a potential confounding factor in soft agar clonogenic assays for tumour cell radiosensitivity.
Authors:
Lawton, P A; Hodgkiss, R J; Eyden, Brian P; Joiner, M C
Abstract:
Soft agar clonogenic assays are considered to be a standard method for measuring tumour cell radiosensitivity and it has been widely reported that fibroblast contamination does not occur. We report here that human fibroblasts can proliferate to form colonies in a modified form of the Courtenay-Mills soft agar clonogenic assay. It was observed that early passage skin fibroblasts could form colonies in soft agar, although the plating efficiencies were reduced compared with growth on plastic. It was demonstrated that normal lung could proliferate in agar with similar plating efficiencies to fresh tumours and that fibroblastic cells were present in these cultures. Characterisation of primary lung tumour cultures also showed that fibroblastic cells were present in these cultures. Characterisation of primary lung tumour cultures also showed that fibroblastic cells were present which lacked epithelial features and which resembled closely the cells found in cultures of normal lung. This is an important finding for workers using soft agar assays to culture human tumour cells and is of interest in understanding the processes of normal growth control of human fibroblasts.
Affiliation:
CRC Gray Laboratory, Mount Vernon Hospital, Northwood, Middlesex, UK.
Citation:
Growth of fibroblasts as a potential confounding factor in soft agar clonogenic assays for tumour cell radiosensitivity. 1994, 32 (3):218-25 Radiother Oncol
Journal:
Radiotherapy and Oncology
Issue Date:
Sep-1994
URI:
http://hdl.handle.net/10541/97065
DOI:
10.1016/0167-8140(94)90021-3
PubMed ID:
7529415
Type:
Article
Language:
en
ISSN:
0167-8140
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorLawton, P Aen
dc.contributor.authorHodgkiss, R Jen
dc.contributor.authorEyden, Brian Pen
dc.contributor.authorJoiner, M Cen
dc.date.accessioned2010-04-21T14:26:32Z-
dc.date.available2010-04-21T14:26:32Z-
dc.date.issued1994-09-
dc.identifier.citationGrowth of fibroblasts as a potential confounding factor in soft agar clonogenic assays for tumour cell radiosensitivity. 1994, 32 (3):218-25 Radiother Oncolen
dc.identifier.issn0167-8140-
dc.identifier.pmid7529415-
dc.identifier.doi10.1016/0167-8140(94)90021-3-
dc.identifier.urihttp://hdl.handle.net/10541/97065-
dc.description.abstractSoft agar clonogenic assays are considered to be a standard method for measuring tumour cell radiosensitivity and it has been widely reported that fibroblast contamination does not occur. We report here that human fibroblasts can proliferate to form colonies in a modified form of the Courtenay-Mills soft agar clonogenic assay. It was observed that early passage skin fibroblasts could form colonies in soft agar, although the plating efficiencies were reduced compared with growth on plastic. It was demonstrated that normal lung could proliferate in agar with similar plating efficiencies to fresh tumours and that fibroblastic cells were present in these cultures. Characterisation of primary lung tumour cultures also showed that fibroblastic cells were present in these cultures. Characterisation of primary lung tumour cultures also showed that fibroblastic cells were present which lacked epithelial features and which resembled closely the cells found in cultures of normal lung. This is an important finding for workers using soft agar assays to culture human tumour cells and is of interest in understanding the processes of normal growth control of human fibroblasts.en
dc.language.isoenen
dc.subjectLung Canceren
dc.subjectCultured Tumour Cellsen
dc.subjectTumour Stem Cell Assayen
dc.subject.meshAgar-
dc.subject.meshCell Division-
dc.subject.meshCells, Cultured-
dc.subject.meshCulture Media-
dc.subject.meshEpithelial Cells-
dc.subject.meshEpithelium-
dc.subject.meshFibroblasts-
dc.subject.meshHumans-
dc.subject.meshKeratins-
dc.subject.meshLung-
dc.subject.meshLung Neoplasms-
dc.subject.meshMicroscopy, Electron-
dc.subject.meshRadiation Tolerance-
dc.subject.meshSkin-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshTumor Stem Cell Assay-
dc.titleGrowth of fibroblasts as a potential confounding factor in soft agar clonogenic assays for tumour cell radiosensitivity.en
dc.typeArticleen
dc.contributor.departmentCRC Gray Laboratory, Mount Vernon Hospital, Northwood, Middlesex, UK.en
dc.identifier.journalRadiotherapy and Oncologyen

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