An E. coli ada transgenic clone of Nicotiana tabacum var. Xanthi has increased sensitivity to the mutagenic action of alkylating agents, maleic hydrazide and gamma-rays.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96973
Title:
An E. coli ada transgenic clone of Nicotiana tabacum var. Xanthi has increased sensitivity to the mutagenic action of alkylating agents, maleic hydrazide and gamma-rays.
Authors:
Velemínský, J; Angelis, K; Babůrek, I; Gichner, T; Satava, J; Bríza, J; Margison, Geoffrey P
Abstract:
Two transgenic clones X3 and X15 of Nicotiana tabacum var. Xanthi, heterozygous in two genes (a1 and a2) for chloroplast differentiation and transformed with the E. coli DNA repair gene ada cloned downstream from the 1' direction of the dual mas promoter, differed in the expression of the ada gene, in the number of copies of integrated T-DNA and in the response to the mutagenic action of alkylating and non-alkylating agents. The X3 genome contained four copies and the X15 genome one copy of T-DNA, nevertheless the expression of the ada gene, measured by the activity of O6-alkylguanine DNA alkyltransferase (ATase), was about six times higher in X15 than in X3. ATase activity in both clones was highest in extracts from callus whereas very low (X15) or no (X3) activity was detected in leaf extracts. This may explain the lack of difference between X15 and non-transformed tobacco (NTX) in the frequency of N-methyl-N-nitrosourea (MNU)-induced somatic mutations in leaves. In contrast, the frequency of somatic mutations in X3 was about 2-5 times higher than in NTX and X15 after the same doses of MNU, methyl methanesulfonate, maleic hydrazide and gamma-rays. Alteration of plant gene(s) essential in mutation pathway(s) by insertion of T-DNA or by somaclonal variation may explain the higher sensitivity of the X3 clone.
Affiliation:
Institute of Experimental Botany, Academy of Sciences, Prague, Czech Republic.
Citation:
An E. coli ada transgenic clone of Nicotiana tabacum var. Xanthi has increased sensitivity to the mutagenic action of alkylating agents, maleic hydrazide and gamma-rays. 1994, 307 (1):193-200 Mutat. Res.
Journal:
Mutation Research
Issue Date:
1-May-1994
URI:
http://hdl.handle.net/10541/96973
PubMed ID:
7513797
Type:
Article
Language:
en
ISSN:
0027-5107
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorVelemínský, Jen
dc.contributor.authorAngelis, Ken
dc.contributor.authorBabůrek, Ien
dc.contributor.authorGichner, Ten
dc.contributor.authorSatava, Jen
dc.contributor.authorBríza, Jen
dc.contributor.authorMargison, Geoffrey Pen
dc.date.accessioned2010-04-21T08:44:07Z-
dc.date.available2010-04-21T08:44:07Z-
dc.date.issued1994-05-01-
dc.identifier.citationAn E. coli ada transgenic clone of Nicotiana tabacum var. Xanthi has increased sensitivity to the mutagenic action of alkylating agents, maleic hydrazide and gamma-rays. 1994, 307 (1):193-200 Mutat. Res.en
dc.identifier.issn0027-5107-
dc.identifier.pmid7513797-
dc.identifier.urihttp://hdl.handle.net/10541/96973-
dc.description.abstractTwo transgenic clones X3 and X15 of Nicotiana tabacum var. Xanthi, heterozygous in two genes (a1 and a2) for chloroplast differentiation and transformed with the E. coli DNA repair gene ada cloned downstream from the 1' direction of the dual mas promoter, differed in the expression of the ada gene, in the number of copies of integrated T-DNA and in the response to the mutagenic action of alkylating and non-alkylating agents. The X3 genome contained four copies and the X15 genome one copy of T-DNA, nevertheless the expression of the ada gene, measured by the activity of O6-alkylguanine DNA alkyltransferase (ATase), was about six times higher in X15 than in X3. ATase activity in both clones was highest in extracts from callus whereas very low (X15) or no (X3) activity was detected in leaf extracts. This may explain the lack of difference between X15 and non-transformed tobacco (NTX) in the frequency of N-methyl-N-nitrosourea (MNU)-induced somatic mutations in leaves. In contrast, the frequency of somatic mutations in X3 was about 2-5 times higher than in NTX and X15 after the same doses of MNU, methyl methanesulfonate, maleic hydrazide and gamma-rays. Alteration of plant gene(s) essential in mutation pathway(s) by insertion of T-DNA or by somaclonal variation may explain the higher sensitivity of the X3 clone.en
dc.language.isoenen
dc.subject.meshAlkylating Agents-
dc.subject.meshBacterial Proteins-
dc.subject.meshBase Sequence-
dc.subject.meshCells, Cultured-
dc.subject.meshCloning, Molecular-
dc.subject.meshDNA, Recombinant-
dc.subject.meshEscherichia coli-
dc.subject.meshEscherichia coli Proteins-
dc.subject.meshGamma Rays-
dc.subject.meshGenes, Bacterial-
dc.subject.meshMaleic Hydrazide-
dc.subject.meshMethyltransferases-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMutagens-
dc.subject.meshO(6)-Methylguanine-DNA Methyltransferase-
dc.subject.meshPlants, Genetically Modified-
dc.subject.meshPlants, Toxic-
dc.subject.meshTobacco-
dc.subject.meshTranscription Factors-
dc.subject.meshTransformation, Genetic-
dc.titleAn E. coli ada transgenic clone of Nicotiana tabacum var. Xanthi has increased sensitivity to the mutagenic action of alkylating agents, maleic hydrazide and gamma-rays.en
dc.typeArticleen
dc.contributor.departmentInstitute of Experimental Botany, Academy of Sciences, Prague, Czech Republic.en
dc.identifier.journalMutation Researchen
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