The role of p53 in spontaneous and radiation-induced apoptosis in the gastrointestinal tract of normal and p53-deficient mice.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96965
Title:
The role of p53 in spontaneous and radiation-induced apoptosis in the gastrointestinal tract of normal and p53-deficient mice.
Authors:
Merritt, Anita J; Potten, Christopher S; Kemp, C J; Hickman, John A; Balmain, A; Lane, D P; Hall, P A
Abstract:
Three h after whole-body irradiation (8 Gy) of C57BL x DBA/2 F1 mice, p53 protein was expressed strongly in the stem cell compartment of the small intestine but at lower levels in the colon. At this time, apoptotic cells were also observed in the stem cell position of the small intestine, with fewer in the colon. In mice without copies of the p53 gene (nulls), the levels of spontaneous apoptosis, in both the small intestine and the colon, were not different from wild-type. Irradiation of the nulls with 8 Gy of gamma-rays failed to induce any further apoptosis: the loss of p53 essentially rendered the epithelial cells, from both the small intestine and the colon, radioresistant. The response of the epithelial stem cells of the small intestine suggests that p53 may play a role in the deletion of damaged cells with carcinogenic potential, whereas this process is limited in the colon.
Affiliation:
CRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, United Kingdom.
Citation:
The role of p53 in spontaneous and radiation-induced apoptosis in the gastrointestinal tract of normal and p53-deficient mice. 1994, 54 (3):614-7 Cancer Res.
Journal:
Cancer Research
Issue Date:
1-Feb-1994
URI:
http://hdl.handle.net/10541/96965
PubMed ID:
8306319
Type:
Article
Language:
en
ISSN:
0008-5472
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMerritt, Anita Jen
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorKemp, C Jen
dc.contributor.authorHickman, John Aen
dc.contributor.authorBalmain, Aen
dc.contributor.authorLane, D Pen
dc.contributor.authorHall, P Aen
dc.date.accessioned2010-04-21T09:29:21Z-
dc.date.available2010-04-21T09:29:21Z-
dc.date.issued1994-02-01-
dc.identifier.citationThe role of p53 in spontaneous and radiation-induced apoptosis in the gastrointestinal tract of normal and p53-deficient mice. 1994, 54 (3):614-7 Cancer Res.en
dc.identifier.issn0008-5472-
dc.identifier.pmid8306319-
dc.identifier.urihttp://hdl.handle.net/10541/96965-
dc.description.abstractThree h after whole-body irradiation (8 Gy) of C57BL x DBA/2 F1 mice, p53 protein was expressed strongly in the stem cell compartment of the small intestine but at lower levels in the colon. At this time, apoptotic cells were also observed in the stem cell position of the small intestine, with fewer in the colon. In mice without copies of the p53 gene (nulls), the levels of spontaneous apoptosis, in both the small intestine and the colon, were not different from wild-type. Irradiation of the nulls with 8 Gy of gamma-rays failed to induce any further apoptosis: the loss of p53 essentially rendered the epithelial cells, from both the small intestine and the colon, radioresistant. The response of the epithelial stem cells of the small intestine suggests that p53 may play a role in the deletion of damaged cells with carcinogenic potential, whereas this process is limited in the colon.en
dc.language.isoenen
dc.subjectColonic Canceren
dc.subjectIntestinal Canceren
dc.subjectTumour Suppressor Protein p53en
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshColon-
dc.subject.meshColonic Neoplasms-
dc.subject.meshDigestive System-
dc.subject.meshDigestive System Physiological Phenomena-
dc.subject.meshEpithelium-
dc.subject.meshIncidence-
dc.subject.meshIntestinal Neoplasms-
dc.subject.meshIntestine, Large-
dc.subject.meshIntestine, Small-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshMice, Inbred DBA-
dc.subject.meshNeoplasms, Radiation-Induced-
dc.subject.meshTime Factors-
dc.subject.meshTumor Suppressor Protein p53-
dc.subject.meshWhole-Body Irradiation-
dc.titleThe role of p53 in spontaneous and radiation-induced apoptosis in the gastrointestinal tract of normal and p53-deficient mice.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, United Kingdom.en
dc.identifier.journalCancer Researchen
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