Analysis of retrotransposon families in genomic DNA by two-dimensional restriction mapping: detection of VL30 insertions in mouse thymic lymphoma.
Affiliation
CRC Department of Gene Regulation, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.Issue Date
1994-10-18
Metadata
Show full item recordAbstract
Reinsertion of mammalian retrotransposable genetic elements is known to be causally associated with tumourigenesis, typically through mechanisms involving insertional deregulation of cellular protooncogene expression. We report here on the application of a two-dimensional restriction mapping-Southern hybridisation approach for analysis of retrotransposon families of low to moderate genetic complexity, which is particularly suited to pairwise comparisons between DNA samples. By using this method, non-constitutional mink-cell-focus-forming type retro-elements were readily detectable in AKR mouse thymic lymphomas against a background of approx. 30 related elements in control DNA. However, in the WEHI 3B myeloid leukaemia cell line, the resolution of two-dimensional mapping permitted detection of only occasional reinsertions of intracisternal A particle retro-elements (genetic complexity: 10(3)). In analysing the VL30 family of retrotransposon (genetic complexity: 150) we developed a strategy for identifying the known transcriptionally active sub-set of these elements in genomic DNA through the generation of an internal, diagnostic restriction fragment. Moreover, in some cases of thymic lymphoma, several candidate re-insertions of VL30 elements were detected, consistent with a suggested role for retrotransposition of this class of element in lymphomagenesis of retroviral aetiology.Citation
Analysis of retrotransposon families in genomic DNA by two-dimensional restriction mapping: detection of VL30 insertions in mouse thymic lymphoma. 1994, 1219 (2):484-92 Biochim. Biophys. ActaJournal
Biochimica et Biophysica ActaPubMed ID
7918646Type
ArticleLanguage
enISSN
0006-3002Collections
Related articles
- Activation and transposition of endogenous retroviral elements in hypomethylation induced tumors in mice.
- Authors: Howard G, Eiges R, Gaudet F, Jaenisch R, Eden A
- Issue date: 2008 Jan 10
- Organization and expression of endogenous virus-like (VL30) DNA sequences in nontransformed and chemically transformed mouse embryo cells in culture.
- Authors: Courtney MG, Schmidt LJ, Getz MJ
- Issue date: 1982 Feb
- Calorie restriction suppresses subgenomic mink cytopathic focus-forming murine leukemia virus transcription and frequency of genomic expression while impairing lymphoma formation.
- Authors: Shields BA, Engelman RW, Fukaura Y, Good RA, Day NK
- Issue date: 1991 Dec 15
- Arsenic induces VL30 retrotransposition: the involvement of oxidative stress and heat-shock protein 70.
- Authors: Markopoulos G, Noutsopoulos D, Mantziou S, Vartholomatos G, Monokrousos N, Angelidis C, Tzavaras T
- Issue date: 2013 Aug
- VL30 retrotransposition signals activation of a caspase-independent and p53-dependent death pathway associated with mitochondrial and lysosomal damage.
- Authors: Noutsopoulos D, Markopoulos G, Vartholomatos G, Kolettas E, Kolaitis N, Tzavaras T
- Issue date: 2010 May