Biological consequences of p160v-abl protein tyrosine kinase activity in a primitive, multipotent haemopoietic cell line.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96921
Title:
Biological consequences of p160v-abl protein tyrosine kinase activity in a primitive, multipotent haemopoietic cell line.
Authors:
Spooncer, Elaine; Fairbairn, Leslie J; Cowling, Graham J; Dexter, T Michael; Whetton, Anthony D; Owen-Lynch, P Jane
Abstract:
A temperature sensitive abl protein tyrosine kinase gene was transferred into a multipotent haemopoietic stem cell line, and the primary biological effects of expression of the gene were examined at the permissive and non-permissive temperatures. Unlike previous studies in factor-dependent cell lines, we found that expression of the functional abl protein tyrosine kinase did not lead to growth autonomy. Furthermore, the cells were still able to undergo terminal myeloid differentiation. However, expression of the functional gene did lead to a delay in maturation with a concomitant increase in cell production, had a modest effect in terms of delayed apoptosis particularly when the cells were maintained at a high cell density, and slightly increased the response to sub-optimal concentrations of IL-3. In many respects, therefore, the effects of abl protein tyrosine kinase in these cells mimics the effect of bcr/abl in primary haemopoietic cells where growth factor independence and an aberrant differentiation profile are relatively late events in clonal evolution and are not intermediate consequences of activation of the abl gene.
Affiliation:
Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
Biological consequences of p160v-abl protein tyrosine kinase activity in a primitive, multipotent haemopoietic cell line. 1994, 8 (4):620-30 Leukemia
Journal:
Leukemia
Issue Date:
Apr-1994
URI:
http://hdl.handle.net/10541/96921
PubMed ID:
8152257
Type:
Article
Language:
en
ISSN:
0887-6924
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSpooncer, Elaineen
dc.contributor.authorFairbairn, Leslie Jen
dc.contributor.authorCowling, Graham Jen
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorWhetton, Anthony Den
dc.contributor.authorOwen-Lynch, P Janeen
dc.date.accessioned2010-04-20T15:33:02Z-
dc.date.available2010-04-20T15:33:02Z-
dc.date.issued1994-04-
dc.identifier.citationBiological consequences of p160v-abl protein tyrosine kinase activity in a primitive, multipotent haemopoietic cell line. 1994, 8 (4):620-30 Leukemiaen
dc.identifier.issn0887-6924-
dc.identifier.pmid8152257-
dc.identifier.urihttp://hdl.handle.net/10541/96921-
dc.description.abstractA temperature sensitive abl protein tyrosine kinase gene was transferred into a multipotent haemopoietic stem cell line, and the primary biological effects of expression of the gene were examined at the permissive and non-permissive temperatures. Unlike previous studies in factor-dependent cell lines, we found that expression of the functional abl protein tyrosine kinase did not lead to growth autonomy. Furthermore, the cells were still able to undergo terminal myeloid differentiation. However, expression of the functional gene did lead to a delay in maturation with a concomitant increase in cell production, had a modest effect in terms of delayed apoptosis particularly when the cells were maintained at a high cell density, and slightly increased the response to sub-optimal concentrations of IL-3. In many respects, therefore, the effects of abl protein tyrosine kinase in these cells mimics the effect of bcr/abl in primary haemopoietic cells where growth factor independence and an aberrant differentiation profile are relatively late events in clonal evolution and are not intermediate consequences of activation of the abl gene.en
dc.language.isoenen
dc.subject.meshApoptosis-
dc.subject.meshCell Count-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Line-
dc.subject.meshDiglycerides-
dc.subject.meshDrug Resistance-
dc.subject.meshGenes, abl-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshInterleukin-3-
dc.subject.meshNeomycin-
dc.subject.meshOncogene Proteins v-abl-
dc.subject.meshPhosphorylation-
dc.subject.meshProtein-Tyrosine Kinases-
dc.subject.meshRetroviridae-
dc.subject.meshTemperature-
dc.titleBiological consequences of p160v-abl protein tyrosine kinase activity in a primitive, multipotent haemopoietic cell line.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalLeukemiaen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.