Cell migration in the small and large bowel shows a strong circadian rhythm.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96207
Title:
Cell migration in the small and large bowel shows a strong circadian rhythm.
Authors:
Qiu, J M; Roberts, Stephen A; Potten, Christopher S
Abstract:
Migration velocity estimates have been determined at each position along the crypt length for both the small and large intestine of the mouse at 6 different times of the day. Measurements also have been made of crypt circumference and length. Dramatic, and significant (P < 0.001), changes in migration velocity as a function of time of day were observed in the small intestine with a maximum 0.84 cell positions (cp) per hour at 0900 h and a minimum of -0.46 cp/h at 1700 h, although the negative velocity was probably artefactual. The 24-h mean velocity rose smoothly as a function of cell position to a peak of 0.45 cp/h at cell position 17 (around the top of the proliferative zone). Much more modest changes were seen in the percent of 3HTdR labelled cells (minimum 30.8%, maximum 38.3%, P < 0.001) and crypt circumference (minimum 16.9 cells, maximum 17.9 cells, P = 0.003). The migration velocity was rather less well determined in the large intestine with a peak in the 24-h mean velocity (0.26 cp/h) occurring at cell position 10. At this position significant circadian variation was detected (minimum -0.39 cp/h, maximum 0.75 cp/h, P = 0.006). Changes were seen in the percent of labelled cells (minimum 9.4%, maximum 22.3%, P < 0.001) and crypt circumference (minimum 18.3 cells, maximum 19.2 cells, P < 0.001). In both tissues it is suggested that the combination of the modest changes in cell proliferation rates in conjunction with the changes in crypt cell number can account for the large amplitude in variation of crypt output, and that the reservoir effects of changes in crypt geometry are an essential part of the process governing the maintenance of intestinal cell numbers.
Affiliation:
CRC Department of Epithelial Biology, Paterson Institute of Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
Citation:
Cell migration in the small and large bowel shows a strong circadian rhythm. 1994, 3 (4):137-48 Epithelial Cell Biol
Journal:
Epithelial Cell Biology
Issue Date:
1994
URI:
http://hdl.handle.net/10541/96207
PubMed ID:
7550605
Type:
Article
Language:
en
ISSN:
0940-9912
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorQiu, J Men
dc.contributor.authorRoberts, Stephen Aen
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2010-04-09T14:51:34Z-
dc.date.available2010-04-09T14:51:34Z-
dc.date.issued1994-
dc.identifier.citationCell migration in the small and large bowel shows a strong circadian rhythm. 1994, 3 (4):137-48 Epithelial Cell Biolen
dc.identifier.issn0940-9912-
dc.identifier.pmid7550605-
dc.identifier.urihttp://hdl.handle.net/10541/96207-
dc.description.abstractMigration velocity estimates have been determined at each position along the crypt length for both the small and large intestine of the mouse at 6 different times of the day. Measurements also have been made of crypt circumference and length. Dramatic, and significant (P < 0.001), changes in migration velocity as a function of time of day were observed in the small intestine with a maximum 0.84 cell positions (cp) per hour at 0900 h and a minimum of -0.46 cp/h at 1700 h, although the negative velocity was probably artefactual. The 24-h mean velocity rose smoothly as a function of cell position to a peak of 0.45 cp/h at cell position 17 (around the top of the proliferative zone). Much more modest changes were seen in the percent of 3HTdR labelled cells (minimum 30.8%, maximum 38.3%, P < 0.001) and crypt circumference (minimum 16.9 cells, maximum 17.9 cells, P = 0.003). The migration velocity was rather less well determined in the large intestine with a peak in the 24-h mean velocity (0.26 cp/h) occurring at cell position 10. At this position significant circadian variation was detected (minimum -0.39 cp/h, maximum 0.75 cp/h, P = 0.006). Changes were seen in the percent of labelled cells (minimum 9.4%, maximum 22.3%, P < 0.001) and crypt circumference (minimum 18.3 cells, maximum 19.2 cells, P < 0.001). In both tissues it is suggested that the combination of the modest changes in cell proliferation rates in conjunction with the changes in crypt cell number can account for the large amplitude in variation of crypt output, and that the reservoir effects of changes in crypt geometry are an essential part of the process governing the maintenance of intestinal cell numbers.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Movement-
dc.subject.meshCircadian Rhythm-
dc.subject.meshColon-
dc.subject.meshDNA-
dc.subject.meshDarkness-
dc.subject.meshEpithelial Cells-
dc.subject.meshEpithelium-
dc.subject.meshIleum-
dc.subject.meshIntestinal Mucosa-
dc.subject.meshIntestine, Large-
dc.subject.meshIntestine, Small-
dc.subject.meshLight-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred Strains-
dc.subject.meshMitotic Index-
dc.subject.meshThymidine-
dc.titleCell migration in the small and large bowel shows a strong circadian rhythm.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Epithelial Biology, Paterson Institute of Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.en
dc.identifier.journalEpithelial Cell Biologyen
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