Delayed-type hypersensitivity response to human papillomavirus type 16 E6 protein in a mouse model.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96200
Title:
Delayed-type hypersensitivity response to human papillomavirus type 16 E6 protein in a mouse model.
Authors:
Chambers, M A; Stacey, Simon N; Arrand, John R; Stanley, M A
Abstract:
A mouse model incorporating the epitheliotropic nature of human papillomavirus (HPV) infections has been used to study an immune response to HPV type 16 (HPV-16) E6 protein in vivo. Using a transplantation technique, a novel immortal keratinocyte cell line expressing the E6 protein has been grafted onto syngeneic mice to re-form a differentiated epithelium overlying a granulation tissue bed. By this approach the presentation of viral antigens to the immune system can be modelled in a way analogous to the natural infection. Here we report a delayed-type hypersensitivity (DTH) reaction in grafted mice challenged intradermally with a recombinant vaccinia virus expressing the HPV-16 E6 protein. The specificity of the response was confirmed by the absence of a DTH reaction to challenge with virus expressing either HPV-16 E7 or L1 protein.
Affiliation:
Department of Pathology, University of Cambridge, U.K.
Citation:
Delayed-type hypersensitivity response to human papillomavirus type 16 E6 protein in a mouse model. 1994, 75 ( Pt 1):165-9 J. Gen. Virol.
Journal:
The Journal of General Virology
Issue Date:
Jan-1994
URI:
http://hdl.handle.net/10541/96200
DOI:
10.1099/0022-1317-75-1-165
PubMed ID:
7906707
Type:
Article
Language:
en
ISSN:
0022-1317
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorChambers, M Aen
dc.contributor.authorStacey, Simon Nen
dc.contributor.authorArrand, John Ren
dc.contributor.authorStanley, M Aen
dc.date.accessioned2010-04-09T13:52:43Z-
dc.date.available2010-04-09T13:52:43Z-
dc.date.issued1994-01-
dc.identifier.citationDelayed-type hypersensitivity response to human papillomavirus type 16 E6 protein in a mouse model. 1994, 75 ( Pt 1):165-9 J. Gen. Virol.en
dc.identifier.issn0022-1317-
dc.identifier.pmid7906707-
dc.identifier.doi10.1099/0022-1317-75-1-165-
dc.identifier.urihttp://hdl.handle.net/10541/96200-
dc.description.abstractA mouse model incorporating the epitheliotropic nature of human papillomavirus (HPV) infections has been used to study an immune response to HPV type 16 (HPV-16) E6 protein in vivo. Using a transplantation technique, a novel immortal keratinocyte cell line expressing the E6 protein has been grafted onto syngeneic mice to re-form a differentiated epithelium overlying a granulation tissue bed. By this approach the presentation of viral antigens to the immune system can be modelled in a way analogous to the natural infection. Here we report a delayed-type hypersensitivity (DTH) reaction in grafted mice challenged intradermally with a recombinant vaccinia virus expressing the HPV-16 E6 protein. The specificity of the response was confirmed by the absence of a DTH reaction to challenge with virus expressing either HPV-16 E7 or L1 protein.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshAntigens, Viral-
dc.subject.meshCD4-Positive T-Lymphocytes-
dc.subject.meshCell Line-
dc.subject.meshHypersensitivity, Delayed-
dc.subject.meshKeratinocytes-
dc.subject.meshMice-
dc.subject.meshMice, Inbred BALB C-
dc.subject.meshOncogene Proteins, Viral-
dc.subject.meshPapillomaviridae-
dc.subject.meshRecombinant Proteins-
dc.subject.meshRepressor Proteins-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshVaccinia virus-
dc.titleDelayed-type hypersensitivity response to human papillomavirus type 16 E6 protein in a mouse model.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathology, University of Cambridge, U.K.en
dc.identifier.journalThe Journal of General Virologyen

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