Evidence for the simultaneous expression of alternatively spliced alkylpurine N-glycosylase transcripts in human tissues and cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96196
Title:
Evidence for the simultaneous expression of alternatively spliced alkylpurine N-glycosylase transcripts in human tissues and cells.
Authors:
Pendlebury, Alizon; Frayling, Ian M; Santibanez-Koref, Mauro F; Margison, Geoffrey P; Rafferty, Joseph A
Abstract:
We have isolated a novel human alkylpurine N-glycosylase (APNG) cDNA from a placental library by screening with an oligonucleotide based on the published sequence of the human liver cDNA encoding this protein. The nucleotide sequences of the two cDNAs were essentially identical, but the 5' untranslated region of the new sequence was truncated and the 5'-terminal 92 nucleotides of the novel cDNA were different, indicating the possibility of alternative transcripts. This region included a portion of the open reading frame, so that the predicted protein was truncated and the seven N-terminal amino acids differed from the published sequence for APNG. PCR amplification of reverse transcribed mRNA, using 5' primers unique to the two cDNAs and a common 3' primer showed that the alternative transcripts can be co-expressed in the same cells and tissues.
Affiliation:
CRC Department of Carcinogenesis, Paterson Institute for Cancer Research, Christie Hospital-NHS Trust, Manchester, UK.
Citation:
Evidence for the simultaneous expression of alternatively spliced alkylpurine N-glycosylase transcripts in human tissues and cells. 1994, 15 (12):2957-60 Carcinogenesis
Journal:
Carcinogenesis
Issue Date:
Dec-1994
URI:
http://hdl.handle.net/10541/96196
DOI:
10.1093/carcin/15.12.2957
PubMed ID:
8001263
Type:
Article
Language:
en
ISSN:
0143-3334
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPendlebury, Alizonen
dc.contributor.authorFrayling, Ian Men
dc.contributor.authorSantibanez-Koref, Mauro Fen
dc.contributor.authorMargison, Geoffrey Pen
dc.contributor.authorRafferty, Joseph Aen
dc.date.accessioned2010-04-09T13:49:39Z-
dc.date.available2010-04-09T13:49:39Z-
dc.date.issued1994-12-
dc.identifier.citationEvidence for the simultaneous expression of alternatively spliced alkylpurine N-glycosylase transcripts in human tissues and cells. 1994, 15 (12):2957-60 Carcinogenesisen
dc.identifier.issn0143-3334-
dc.identifier.pmid8001263-
dc.identifier.doi10.1093/carcin/15.12.2957-
dc.identifier.urihttp://hdl.handle.net/10541/96196-
dc.description.abstractWe have isolated a novel human alkylpurine N-glycosylase (APNG) cDNA from a placental library by screening with an oligonucleotide based on the published sequence of the human liver cDNA encoding this protein. The nucleotide sequences of the two cDNAs were essentially identical, but the 5' untranslated region of the new sequence was truncated and the 5'-terminal 92 nucleotides of the novel cDNA were different, indicating the possibility of alternative transcripts. This region included a portion of the open reading frame, so that the predicted protein was truncated and the seven N-terminal amino acids differed from the published sequence for APNG. PCR amplification of reverse transcribed mRNA, using 5' primers unique to the two cDNAs and a common 3' primer showed that the alternative transcripts can be co-expressed in the same cells and tissues.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshBase Sequence-
dc.subject.meshCell Line-
dc.subject.meshDNA, Complementary-
dc.subject.meshGene Expression Regulation, Enzymologic-
dc.subject.meshHumans-
dc.subject.meshIsoenzymes-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshOrgan Specificity-
dc.subject.meshRNA Splicing-
dc.subject.meshRNA, Heterogeneous Nuclear-
dc.subject.meshRNA, Messenger-
dc.subject.meshSequence Alignment-
dc.subject.meshSequence Homology, Amino Acid-
dc.subject.meshSubstrate Specificity-
dc.titleEvidence for the simultaneous expression of alternatively spliced alkylpurine N-glycosylase transcripts in human tissues and cells.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Carcinogenesis, Paterson Institute for Cancer Research, Christie Hospital-NHS Trust, Manchester, UK.en
dc.identifier.journalCarcinogenesisen
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