2.50
Hdl Handle:
http://hdl.handle.net/10541/96144
Title:
Expression of pokeweed lectin binding in murine intestinal Paneth cells.
Authors:
Evans, Gareth S; Chwalinski, S; Owen, Gary; Booth, Catherine; Singh, A; Potten, Christopher S
Abstract:
Pokeweed (Phytolacca americana) lectin was found to stain the secretory granules in epithelial Paneth cells of small intestine in mice and rats. The distribution of Paneth cells stained with this lectin was identical to that obtained by another immunohistochemical marker for lysozyme. However, in comparison with other immunohistochemical markers, Pokeweed lectin is a more robust method for identifying Paneth cells in histological sections and for studying their secretory granules. Co-expression of the Pokeweed lectin binding sites in some oligomucous cells within the crypts suggested a close developmental link between these two cell types. Only one other non-epithelial cell type was stained by this lectin, and these were migratory lymphocytes found within the villus epithelium and lamina propria. Approximately 20% of these lymphocyte cells were also positive for the expression of CD3+. Pokeweed lectin was therefore used to study changes in the frequency of Paneth cells and intra-epithelial lymphocytes in normal and immunologically compromised animals (following infection with a parasite worm Trichuris muris and in a model of graft-versus-host rejection). This study confirmed that the population of Paneth cells turns over slowly even during conditions of inflammation.
Affiliation:
CRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
Citation:
Expression of pokeweed lectin binding in murine intestinal Paneth cells. 1994, 3 (1):7-15 Epithelial Cell Biol
Journal:
Epithelial Cell Biology
Issue Date:
Jan-1994
URI:
http://hdl.handle.net/10541/96144
PubMed ID:
7514935
Type:
Article
Language:
en
ISSN:
0940-9912
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorEvans, Gareth Sen
dc.contributor.authorChwalinski, Sen
dc.contributor.authorOwen, Garyen
dc.contributor.authorBooth, Catherineen
dc.contributor.authorSingh, Aen
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2010-04-09T12:11:59Z-
dc.date.available2010-04-09T12:11:59Z-
dc.date.issued1994-01-
dc.identifier.citationExpression of pokeweed lectin binding in murine intestinal Paneth cells. 1994, 3 (1):7-15 Epithelial Cell Biolen
dc.identifier.issn0940-9912-
dc.identifier.pmid7514935-
dc.identifier.urihttp://hdl.handle.net/10541/96144-
dc.description.abstractPokeweed (Phytolacca americana) lectin was found to stain the secretory granules in epithelial Paneth cells of small intestine in mice and rats. The distribution of Paneth cells stained with this lectin was identical to that obtained by another immunohistochemical marker for lysozyme. However, in comparison with other immunohistochemical markers, Pokeweed lectin is a more robust method for identifying Paneth cells in histological sections and for studying their secretory granules. Co-expression of the Pokeweed lectin binding sites in some oligomucous cells within the crypts suggested a close developmental link between these two cell types. Only one other non-epithelial cell type was stained by this lectin, and these were migratory lymphocytes found within the villus epithelium and lamina propria. Approximately 20% of these lymphocyte cells were also positive for the expression of CD3+. Pokeweed lectin was therefore used to study changes in the frequency of Paneth cells and intra-epithelial lymphocytes in normal and immunologically compromised animals (following infection with a parasite worm Trichuris muris and in a model of graft-versus-host rejection). This study confirmed that the population of Paneth cells turns over slowly even during conditions of inflammation.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCarbohydrate Metabolism-
dc.subject.meshCell Differentiation-
dc.subject.meshCytoplasmic Granules-
dc.subject.meshFemale-
dc.subject.meshGraft vs Host Disease-
dc.subject.meshHistocytochemistry-
dc.subject.meshHumans-
dc.subject.meshIntestinal Mucosa-
dc.subject.meshIntestine, Small-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshMice, Inbred DBA-
dc.subject.meshPokeweed Mitogens-
dc.subject.meshRats-
dc.subject.meshRats, Wistar-
dc.subject.meshStaining and Labeling-
dc.subject.meshTrichuriasis-
dc.titleExpression of pokeweed lectin binding in murine intestinal Paneth cells.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.en
dc.identifier.journalEpithelial Cell Biologyen
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