Heparin stimulates the proliferation of intestinal epithelial cells in primary culture.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96139
Title:
Heparin stimulates the proliferation of intestinal epithelial cells in primary culture.
Authors:
Flint, Neil; Cove, F L; Evans, Gareth S
Abstract:
Heparin is a sulphated glycosaminoglycan derived from mast cells and has a number of functions including the inhibition of proliferation in several cell types and interactions with a range of heparin-binding growth factors. We report that heparin is a trophic factor in primary cultures of rat small intestinal epithelium. Heparin elicits a dose-dependent increase in epithelial proliferation and inhibits the growth of associated mesenchyme. The trophic effect of this molecule is not reproduced by other glycosaminoglycans including heparan sulphate but is dependent upon extensive molecular sulphation. Highly sulphated polysaccharides that are structurally unrelated to heparin (e.g. dextran sulphate and pentosan polysulphate) also stimulate epithelial proliferation in primary cultures. Heparin may act by the potentiation of mesenchyme-derived heparin-binding growth factors and these data suggest an in vivo role for mast cell-derived heparin in mucosal wound regeneration.
Affiliation:
Department of Epithelial Biology, Paterson Institute for Cancer Research, Withington, Manchester, UK.
Citation:
Heparin stimulates the proliferation of intestinal epithelial cells in primary culture. 1994, 107 ( Pt 2):401-11 J. Cell. Sci.
Journal:
Journal of Cell Science
Issue Date:
Feb-1994
URI:
http://hdl.handle.net/10541/96139
PubMed ID:
8207071
Type:
Article
Language:
en
ISSN:
0021-9533
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorFlint, Neilen
dc.contributor.authorCove, F Len
dc.contributor.authorEvans, Gareth Sen
dc.date.accessioned2010-04-09T11:27:24Z-
dc.date.available2010-04-09T11:27:24Z-
dc.date.issued1994-02-
dc.identifier.citationHeparin stimulates the proliferation of intestinal epithelial cells in primary culture. 1994, 107 ( Pt 2):401-11 J. Cell. Sci.en
dc.identifier.issn0021-9533-
dc.identifier.pmid8207071-
dc.identifier.urihttp://hdl.handle.net/10541/96139-
dc.description.abstractHeparin is a sulphated glycosaminoglycan derived from mast cells and has a number of functions including the inhibition of proliferation in several cell types and interactions with a range of heparin-binding growth factors. We report that heparin is a trophic factor in primary cultures of rat small intestinal epithelium. Heparin elicits a dose-dependent increase in epithelial proliferation and inhibits the growth of associated mesenchyme. The trophic effect of this molecule is not reproduced by other glycosaminoglycans including heparan sulphate but is dependent upon extensive molecular sulphation. Highly sulphated polysaccharides that are structurally unrelated to heparin (e.g. dextran sulphate and pentosan polysulphate) also stimulate epithelial proliferation in primary cultures. Heparin may act by the potentiation of mesenchyme-derived heparin-binding growth factors and these data suggest an in vivo role for mast cell-derived heparin in mucosal wound regeneration.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBinding Sites-
dc.subject.meshBiological Transport, Active-
dc.subject.meshCell Division-
dc.subject.meshCells, Cultured-
dc.subject.meshEpithelial Cells-
dc.subject.meshEpithelium-
dc.subject.meshHeparin-
dc.subject.meshIntestine, Small-
dc.subject.meshMesoderm-
dc.subject.meshMolecular Structure-
dc.subject.meshProteoglycans-
dc.subject.meshRats-
dc.titleHeparin stimulates the proliferation of intestinal epithelial cells in primary culture.en
dc.typeArticleen
dc.contributor.departmentDepartment of Epithelial Biology, Paterson Institute for Cancer Research, Withington, Manchester, UK.en
dc.identifier.journalJournal of Cell Scienceen
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