Identification of a naturally processed HLA A0201-restricted viral peptide from cells expressing human papillomavirus type 16 E6 oncoprotein.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96138
Title:
Identification of a naturally processed HLA A0201-restricted viral peptide from cells expressing human papillomavirus type 16 E6 oncoprotein.
Authors:
Bartholomew, Jennifer S; Stacey, Simon N; Coles, B; Burt, Deborah J; Arrand, John R; Stern, Peter L
Abstract:
Human papillomavirus (HPV) DNA encoding the oncogenic proteins E6 and E7 is usually retained in cervical carcinomas, implicating these proteins as potential target antigens for immune recognition in this virally associated tumor. We have characterized endogenously processed peptides eluted from major histocompatibility complex class I molecules in cells infected with a recombinant vaccinia expressing the HPV-16 E6 oncoprotein. The reverse-phase chromatography profile of peptides eluted from isolated HLA-A0201 molecules in cells expressing the E6 oncoprotein differs from that of cells not expressing E6. Sequential Edman degradation of novel peaks found in the peptide profiles from cells expressing HPV-16 E6 led to the identification of a naturally processed HLA-A0201-restricted E6 peptide of sequence KLPQLCTEL. This approach has allowed the identification of a viral peptide which is processed and presented by cells expressing the E6 oncoprotein and is a likely target for cytotoxic T lymphocyte recognition in HLA-A0201-positive patients.
Affiliation:
Department of Immunology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, GB.
Citation:
Identification of a naturally processed HLA A0201-restricted viral peptide from cells expressing human papillomavirus type 16 E6 oncoprotein. 1994, 24 (12):3175-9 Eur. J. Immunol.
Journal:
European Journal of Immunology
Issue Date:
Dec-1994
URI:
http://hdl.handle.net/10541/96138
DOI:
10.1002/eji.1830241239
PubMed ID:
7805746
Type:
Article
Language:
en
ISSN:
0014-2980
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBartholomew, Jennifer Sen
dc.contributor.authorStacey, Simon Nen
dc.contributor.authorColes, Ben
dc.contributor.authorBurt, Deborah Jen
dc.contributor.authorArrand, John Ren
dc.contributor.authorStern, Peter Len
dc.date.accessioned2010-04-09T11:21:28Z-
dc.date.available2010-04-09T11:21:28Z-
dc.date.issued1994-12-
dc.identifier.citationIdentification of a naturally processed HLA A0201-restricted viral peptide from cells expressing human papillomavirus type 16 E6 oncoprotein. 1994, 24 (12):3175-9 Eur. J. Immunol.en
dc.identifier.issn0014-2980-
dc.identifier.pmid7805746-
dc.identifier.doi10.1002/eji.1830241239-
dc.identifier.urihttp://hdl.handle.net/10541/96138-
dc.description.abstractHuman papillomavirus (HPV) DNA encoding the oncogenic proteins E6 and E7 is usually retained in cervical carcinomas, implicating these proteins as potential target antigens for immune recognition in this virally associated tumor. We have characterized endogenously processed peptides eluted from major histocompatibility complex class I molecules in cells infected with a recombinant vaccinia expressing the HPV-16 E6 oncoprotein. The reverse-phase chromatography profile of peptides eluted from isolated HLA-A0201 molecules in cells expressing the E6 oncoprotein differs from that of cells not expressing E6. Sequential Edman degradation of novel peaks found in the peptide profiles from cells expressing HPV-16 E6 led to the identification of a naturally processed HLA-A0201-restricted E6 peptide of sequence KLPQLCTEL. This approach has allowed the identification of a viral peptide which is processed and presented by cells expressing the E6 oncoprotein and is a likely target for cytotoxic T lymphocyte recognition in HLA-A0201-positive patients.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAntigens, Viral-
dc.subject.meshHLA-A Antigens-
dc.subject.meshHumans-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshOncogene Proteins, Viral-
dc.subject.meshPapillomaviridae-
dc.subject.meshPeptides-
dc.subject.meshProtein Binding-
dc.subject.meshRecombinant Proteins-
dc.subject.meshRepressor Proteins-
dc.titleIdentification of a naturally processed HLA A0201-restricted viral peptide from cells expressing human papillomavirus type 16 E6 oncoprotein.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, GB.en
dc.identifier.journalEuropean Journal of Immunologyen

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