Human cytotoxic T lymphocytes stimulated by endogenously processed human papillomavirus type 11 E7 recognize a peptide containing a HLA-A2 (A*0201) motif.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96137
Title:
Human cytotoxic T lymphocytes stimulated by endogenously processed human papillomavirus type 11 E7 recognize a peptide containing a HLA-A2 (A*0201) motif.
Authors:
Tarpey, I; Stacey, Simon N; Hickling, J; Birley, H D; Renton, A; McIndoe, A; Davies, D H
Abstract:
Cytotoxic T lymphocytes (CTL) may play an important role in the control of human papillomavirus (HPV)-induced anogenital neoplasias, but have been difficult to study owing to the difficulty in obtaining sufficient quantities of infectious virus. To address this we have stimulated human HPV-specific CTL in vitro using low-density cells (LDC) from peripheral blood mononuclear cells (PBMC). Low-density cells were used to present synthetic peptides, or endogenously processed peptides expressed from recombinant vaccinia viruses, to high-density PBMC (predominantly lymphocytes) for 6 days. Cytotoxic T lymphocytes stimulated with endogenously processed HPV 11 E7 recognized the synthetic HLA-A2 (A*0201) motif-containing nonamer, 4-12. In reciprocal experiments, CTL stimulated with this peptide in vitro recognized targets expressing endogenously processed E7. The responses in each case were A2 restricted and peptide specific. Two additional A2 motif-containing nonamers from HPV 6b E7 (21-30 and 47-55) also elicited peptide-specific, A2-restricted CTL. The data illustrate the potential that in vitro stimulation with LDC has in understanding CTL responses to experimentally problematic viral systems such as HPV, and may offer a route to specific immunotherapy of HPV-associated lesions.
Affiliation:
Division of Life Sciences, King's College, London, U.K.
Citation:
Human cytotoxic T lymphocytes stimulated by endogenously processed human papillomavirus type 11 E7 recognize a peptide containing a HLA-A2 (A*0201) motif. 1994, 81 (2):222-7 Immunology
Journal:
Immunology
Issue Date:
Feb-1994
URI:
http://hdl.handle.net/10541/96137
PubMed ID:
8157270
Type:
Article
Language:
en
ISSN:
0019-2805
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorTarpey, Ien
dc.contributor.authorStacey, Simon Nen
dc.contributor.authorHickling, Jen
dc.contributor.authorBirley, H Den
dc.contributor.authorRenton, Aen
dc.contributor.authorMcIndoe, Aen
dc.contributor.authorDavies, D Hen
dc.date.accessioned2010-04-09T11:04:14Z-
dc.date.available2010-04-09T11:04:14Z-
dc.date.issued1994-02-
dc.identifier.citationHuman cytotoxic T lymphocytes stimulated by endogenously processed human papillomavirus type 11 E7 recognize a peptide containing a HLA-A2 (A*0201) motif. 1994, 81 (2):222-7 Immunologyen
dc.identifier.issn0019-2805-
dc.identifier.pmid8157270-
dc.identifier.urihttp://hdl.handle.net/10541/96137-
dc.description.abstractCytotoxic T lymphocytes (CTL) may play an important role in the control of human papillomavirus (HPV)-induced anogenital neoplasias, but have been difficult to study owing to the difficulty in obtaining sufficient quantities of infectious virus. To address this we have stimulated human HPV-specific CTL in vitro using low-density cells (LDC) from peripheral blood mononuclear cells (PBMC). Low-density cells were used to present synthetic peptides, or endogenously processed peptides expressed from recombinant vaccinia viruses, to high-density PBMC (predominantly lymphocytes) for 6 days. Cytotoxic T lymphocytes stimulated with endogenously processed HPV 11 E7 recognized the synthetic HLA-A2 (A*0201) motif-containing nonamer, 4-12. In reciprocal experiments, CTL stimulated with this peptide in vitro recognized targets expressing endogenously processed E7. The responses in each case were A2 restricted and peptide specific. Two additional A2 motif-containing nonamers from HPV 6b E7 (21-30 and 47-55) also elicited peptide-specific, A2-restricted CTL. The data illustrate the potential that in vitro stimulation with LDC has in understanding CTL responses to experimentally problematic viral systems such as HPV, and may offer a route to specific immunotherapy of HPV-associated lesions.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAntigens, Viral-
dc.subject.meshBlotting, Northern-
dc.subject.meshCells, Cultured-
dc.subject.meshHLA-A2 Antigen-
dc.subject.meshHumans-
dc.subject.meshLeukocytes, Mononuclear-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPapillomaviridae-
dc.subject.meshPeptides-
dc.subject.meshRecombination, Genetic-
dc.subject.meshT-Lymphocytes, Cytotoxic-
dc.subject.meshVaccinia virus-
dc.titleHuman cytotoxic T lymphocytes stimulated by endogenously processed human papillomavirus type 11 E7 recognize a peptide containing a HLA-A2 (A*0201) motif.en
dc.typeArticleen
dc.contributor.departmentDivision of Life Sciences, King's College, London, U.K.en
dc.identifier.journalImmunologyen

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