Isolation of a cDNA encoding 5T4 oncofetal trophoblast glycoprotein. An antigen associated with metastasis contains leucine-rich repeats.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96136
Title:
Isolation of a cDNA encoding 5T4 oncofetal trophoblast glycoprotein. An antigen associated with metastasis contains leucine-rich repeats.
Authors:
Myers, Kevin A; Rahi-Saund, Veena; Davison, M D; Young, J A; Cheater, Amanada J; Stern, Peter L
Abstract:
The monoclonal antibody 5T4 defines a human oncotrophoblast antigen expressed by a variety of carcinomas but with a restricted pattern of expression in normal adult tissues. The 5T4 antigen has been isolated from term placenta as a 72-kDa glycoprotein consisting of a 42-kDa core protein with extensive N-linked glycosylation. A cDNA has been isolated from a human placental library using pools of oligonucleotides based on amino acid sequence obtained from purified 5T4 molecules. The predicted open reading frame encodes a protein of 420 amino acids with a molecular mass of 46 kDa and 8 potential N-glycosylation sites. There are N- and C-terminal hydrophobic segments corresponding to putative signal and membrane anchorage sequences, respectively. Northern analysis has demonstrated a major 2.5-kilobase mRNA present in cell lines serologically reactive with the monoclonal antibody 5T4. Comparison of the 5T4 protein sequence with current sequence data bases has identified the presence of leucine-rich repeats, which are found in a variety of proteins from yeast, insects, and mammals. The 5T4 antigen expression is strongly associated with metastasis in colorectal and gastric cancer, and, hence, the possible functions of the gene product and its relationship to tumor growth and progression are discussed.
Affiliation:
Cancer Research Campaign Department of Immunology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.
Citation:
Isolation of a cDNA encoding 5T4 oncofetal trophoblast glycoprotein. An antigen associated with metastasis contains leucine-rich repeats. 1994, 269 (12):9319-24 J. Biol. Chem.
Journal:
The Journal of Biological Chemistry
Issue Date:
25-Mar-1994
URI:
http://hdl.handle.net/10541/96136
PubMed ID:
8132670
Type:
Article
Language:
en
ISSN:
0021-9258
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMyers, Kevin Aen
dc.contributor.authorRahi-Saund, Veenaen
dc.contributor.authorDavison, M Den
dc.contributor.authorYoung, J Aen
dc.contributor.authorCheater, Amanada Jen
dc.contributor.authorStern, Peter Len
dc.date.accessioned2010-04-09T11:02:29Z-
dc.date.available2010-04-09T11:02:29Z-
dc.date.issued1994-03-25-
dc.identifier.citationIsolation of a cDNA encoding 5T4 oncofetal trophoblast glycoprotein. An antigen associated with metastasis contains leucine-rich repeats. 1994, 269 (12):9319-24 J. Biol. Chem.en
dc.identifier.issn0021-9258-
dc.identifier.pmid8132670-
dc.identifier.urihttp://hdl.handle.net/10541/96136-
dc.description.abstractThe monoclonal antibody 5T4 defines a human oncotrophoblast antigen expressed by a variety of carcinomas but with a restricted pattern of expression in normal adult tissues. The 5T4 antigen has been isolated from term placenta as a 72-kDa glycoprotein consisting of a 42-kDa core protein with extensive N-linked glycosylation. A cDNA has been isolated from a human placental library using pools of oligonucleotides based on amino acid sequence obtained from purified 5T4 molecules. The predicted open reading frame encodes a protein of 420 amino acids with a molecular mass of 46 kDa and 8 potential N-glycosylation sites. There are N- and C-terminal hydrophobic segments corresponding to putative signal and membrane anchorage sequences, respectively. Northern analysis has demonstrated a major 2.5-kilobase mRNA present in cell lines serologically reactive with the monoclonal antibody 5T4. Comparison of the 5T4 protein sequence with current sequence data bases has identified the presence of leucine-rich repeats, which are found in a variety of proteins from yeast, insects, and mammals. The 5T4 antigen expression is strongly associated with metastasis in colorectal and gastric cancer, and, hence, the possible functions of the gene product and its relationship to tumor growth and progression are discussed.en
dc.language.isoenen
dc.subjectCancer Antigensen
dc.subjectCancer Metastasisen
dc.subjectBiological Tumour Markersen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAntigens, Neoplasm-
dc.subject.meshBase Sequence-
dc.subject.meshChromosome Mapping-
dc.subject.meshChromosomes, Human, Pair 6-
dc.subject.meshCloning, Molecular-
dc.subject.meshConsensus Sequence-
dc.subject.meshDNA Primers-
dc.subject.meshDNA, Complementary-
dc.subject.meshGene Expression-
dc.subject.meshHumans-
dc.subject.meshLeucine-
dc.subject.meshMembrane Glycoproteins-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshNeoplasm Metastasis-
dc.subject.meshRNA, Messenger-
dc.subject.meshRepetitive Sequences, Nucleic Acid-
dc.subject.meshSequence Alignment-
dc.subject.meshSequence Homology, Amino Acid-
dc.subject.meshTrophoblasts-
dc.subject.meshTumor Markers, Biological-
dc.titleIsolation of a cDNA encoding 5T4 oncofetal trophoblast glycoprotein. An antigen associated with metastasis contains leucine-rich repeats.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Immunology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.en
dc.identifier.journalThe Journal of Biological Chemistryen
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