Mutants of a multipotent hematopoietic cell line blocked in GM-CSF-induced differentiation are leukemogenic in vivo.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96117
Title:
Mutants of a multipotent hematopoietic cell line blocked in GM-CSF-induced differentiation are leukemogenic in vivo.
Authors:
Just, Ursula; Spooncer, Elaine; Löhler, J; Stocking, C; Ostertag, W; Dexter, T Michael
Abstract:
FDCP-Mix cells infected with a retroviral vector expressing the GM-CSF gene (GMV-FDCP-Mix) self-renew in the presence of interleukin-3 (IL-3), are multipotent, and undergo differentiation into granulocytes and macrophages coupled with clonal extinction after removal of IL-3. Mutants of GMV-FDCP-Mix were isolated that escape clonal extinction after differentiation induction by the autocrine secreted GM-CSF. Some of these mutant clones have a blast cell morphology and are blocked in differentiation, whereas others exhibit all stages of granulocyte and macrophage differentiation without clonal extinction. In contrast to the parental GMV-FDCP-Mix cells, all the mutants tested were leukemogenic when injected into syngeneic mice. Depending on the in vitro differentiation capacity of the transplanted mutant cell lines, the animals developed undifferentiated blast cell leukemias or CML-like syndromes. Thus, these mutant cell lines can be used to define the cooperating steps in autocrine myeloid leukemia.
Affiliation:
Paterson Institute for Cancer Research, Manchester, UK.
Citation:
Mutants of a multipotent hematopoietic cell line blocked in GM-CSF-induced differentiation are leukemogenic in vivo. 1994, 22 (9):933-40 Exp. Hematol.
Journal:
Experimental Hematology
Issue Date:
Aug-1994
URI:
http://hdl.handle.net/10541/96117
PubMed ID:
8062891
Type:
Article
Language:
en
ISSN:
0301-472X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorJust, Ursulaen
dc.contributor.authorSpooncer, Elaineen
dc.contributor.authorLöhler, Jen
dc.contributor.authorStocking, Cen
dc.contributor.authorOstertag, Wen
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2010-04-09T10:49:31Z-
dc.date.available2010-04-09T10:49:31Z-
dc.date.issued1994-08-
dc.identifier.citationMutants of a multipotent hematopoietic cell line blocked in GM-CSF-induced differentiation are leukemogenic in vivo. 1994, 22 (9):933-40 Exp. Hematol.en
dc.identifier.issn0301-472X-
dc.identifier.pmid8062891-
dc.identifier.urihttp://hdl.handle.net/10541/96117-
dc.description.abstractFDCP-Mix cells infected with a retroviral vector expressing the GM-CSF gene (GMV-FDCP-Mix) self-renew in the presence of interleukin-3 (IL-3), are multipotent, and undergo differentiation into granulocytes and macrophages coupled with clonal extinction after removal of IL-3. Mutants of GMV-FDCP-Mix were isolated that escape clonal extinction after differentiation induction by the autocrine secreted GM-CSF. Some of these mutant clones have a blast cell morphology and are blocked in differentiation, whereas others exhibit all stages of granulocyte and macrophage differentiation without clonal extinction. In contrast to the parental GMV-FDCP-Mix cells, all the mutants tested were leukemogenic when injected into syngeneic mice. Depending on the in vitro differentiation capacity of the transplanted mutant cell lines, the animals developed undifferentiated blast cell leukemias or CML-like syndromes. Thus, these mutant cell lines can be used to define the cooperating steps in autocrine myeloid leukemia.en
dc.language.isoenen
dc.subjectCancerous Cell Transformationen
dc.subjectCancer DNAen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subjectLeukaemiaen
dc.subject.meshAnimals-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Line-
dc.subject.meshCell Transformation, Neoplastic-
dc.subject.meshDNA, Neoplasm-
dc.subject.meshGene Expression Regulation-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshGranulocytes-
dc.subject.meshHematopoiesis-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshInterleukin-3-
dc.subject.meshLeukemia, Myelogenous, Chronic, BCR-ABL Positive-
dc.subject.meshMacrophages-
dc.subject.meshMice-
dc.subject.meshMutation-
dc.titleMutants of a multipotent hematopoietic cell line blocked in GM-CSF-induced differentiation are leukemogenic in vivo.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalExperimental Hematologyen
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