Naloxone interferes with granulocytopoiesis in long-term cultures of mouse bone marrow; buffering by the stromal layer.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96116
Title:
Naloxone interferes with granulocytopoiesis in long-term cultures of mouse bone marrow; buffering by the stromal layer.
Authors:
Krizanac-Bengez, L; Boranić, M; Testa, Nydia G; Kardum, I
Abstract:
Long-term cultures of mouse bone marrow cells were treated with naloxone, starting at the time of culture initiation or in the 2nd or 4th week of culture. Cell proliferation was suppressed and the ratio of immature and mature granulocytes to macrophages diminished by naloxone treatment. The effect depended on the timing of naloxone addition to the cultures and on its concentration, with a bell-shaped dose-response curve. High and low concentrations of naloxone (10(-4), 10(-6), 10(-14) M) interfered with hematopoiesis more strongly than the intermediate concentrations (10(-8) to 10(-12) M). Early cultures lacking the stromal layer were more sensitive to naloxone than the cultures with established stroma. The bell-shaped dose-response curve has been attributed to an interplay of specific (opioid-receptor-mediated) and nonspecific mechanisms. Opioidergic mechanisms apparently participate in the regulation of hematopoiesis.
Affiliation:
Ruder Bosković Institute, Zagreb, Croatia.
Citation:
Naloxone interferes with granulocytopoiesis in long-term cultures of mouse bone marrow; buffering by the stromal layer. 1994, 194 (6):375-82 Res Exp Med (Berl)
Journal:
Research in Experimental Medicine
Issue Date:
1994
URI:
http://hdl.handle.net/10541/96116
DOI:
10.1007/BF02576400
PubMed ID:
7716345
Type:
Article
Language:
en
ISSN:
0300-9130
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorKrizanac-Bengez, Len
dc.contributor.authorBoranić, Men
dc.contributor.authorTesta, Nydia Gen
dc.contributor.authorKardum, Ien
dc.date.accessioned2010-04-09T10:46:06Z-
dc.date.available2010-04-09T10:46:06Z-
dc.date.issued1994-
dc.identifier.citationNaloxone interferes with granulocytopoiesis in long-term cultures of mouse bone marrow; buffering by the stromal layer. 1994, 194 (6):375-82 Res Exp Med (Berl)en
dc.identifier.issn0300-9130-
dc.identifier.pmid7716345-
dc.identifier.doi10.1007/BF02576400-
dc.identifier.urihttp://hdl.handle.net/10541/96116-
dc.description.abstractLong-term cultures of mouse bone marrow cells were treated with naloxone, starting at the time of culture initiation or in the 2nd or 4th week of culture. Cell proliferation was suppressed and the ratio of immature and mature granulocytes to macrophages diminished by naloxone treatment. The effect depended on the timing of naloxone addition to the cultures and on its concentration, with a bell-shaped dose-response curve. High and low concentrations of naloxone (10(-4), 10(-6), 10(-14) M) interfered with hematopoiesis more strongly than the intermediate concentrations (10(-8) to 10(-12) M). Early cultures lacking the stromal layer were more sensitive to naloxone than the cultures with established stroma. The bell-shaped dose-response curve has been attributed to an interplay of specific (opioid-receptor-mediated) and nonspecific mechanisms. Opioidergic mechanisms apparently participate in the regulation of hematopoiesis.en
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subject.meshAnimals-
dc.subject.meshBone Marrow Cells-
dc.subject.meshBuffers-
dc.subject.meshCell Division-
dc.subject.meshCells, Cultured-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshFemale-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshGranulocytes-
dc.subject.meshHematopoiesis-
dc.subject.meshLeukocyte Count-
dc.subject.meshLongitudinal Studies-
dc.subject.meshMacrophages-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshMice, Inbred DBA-
dc.subject.meshNaloxone-
dc.titleNaloxone interferes with granulocytopoiesis in long-term cultures of mouse bone marrow; buffering by the stromal layer.en
dc.typeArticleen
dc.contributor.departmentRuder Bosković Institute, Zagreb, Croatia.en
dc.identifier.journalResearch in Experimental Medicineen
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