O6-alkylguanine-DNA alkyltransferase activity in schistosomiasis-associated human bladder cancer.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96111
Title:
O6-alkylguanine-DNA alkyltransferase activity in schistosomiasis-associated human bladder cancer.
Authors:
Badawi, A F; Cooper, Donald P; Mostafa, M H; Aboul-Azm, T; Barnard, R J; Margison, Geoffrey P; O'Connor, Peter J
Abstract:
O6-Alkylguanine-DNA-alkyltransferase (ATase) activity was measured in extracts of 55 bladder tissue samples (46 tumour and nine uninvolved mucosal tissue) from Egyptian patients with schistosome-associated bladder carcinoma. Activity varied from 2.0 to 16.2 fmole ATase/microgram DNA (mean +/- S.D.; 5.6 +/- 4.0) or from 28 to 351 fmole ATase/mg (117 +/- 71). ATase levels in schistosome-associated bladder cancer tissues (5.6 +/- 4.0 fmole ATase/microgram DNA) tended to be lower than those observed in normal human bladder mucosal tissue (8.5 +/- 4.4 fmole ATase/microgram DNA). In a previous study (Badawi et al., Carcinogenesis, 1992, 13, 877-881) DNA-alkylation damage (O6-methyldeoxyguanosine) was found in 44/46 of these schistosome-associated bladder cancer samples at levels ranging from 0.012 to 0.485 mumole O6-MedG/mole deoxyguanosine. We now report an inverse correlation between the levels of methylation damage and ATase activity (r = -0.67; P < 0.001). These observations encourage further investigations of the possible role of environmental alkylating agents in the aetiology of early bladder cancer associated with schistosomiasis.
Affiliation:
National Center for Toxicology, Jefferson, Arkansas.
Citation:
O6-alkylguanine-DNA alkyltransferase activity in schistosomiasis-associated human bladder cancer. 1994, 30A (9):1314-9 Eur. J. Cancer
Journal:
European Journal of Cancer
Issue Date:
1994
URI:
http://hdl.handle.net/10541/96111
DOI:
10.1016/0959-8049(94)90180-5
PubMed ID:
7999420
Type:
Article
Language:
en
ISSN:
0959-8049
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBadawi, A Fen
dc.contributor.authorCooper, Donald Pen
dc.contributor.authorMostafa, M Hen
dc.contributor.authorAboul-Azm, Ten
dc.contributor.authorBarnard, R Jen
dc.contributor.authorMargison, Geoffrey Pen
dc.contributor.authorO'Connor, Peter Jen
dc.date.accessioned2010-04-09T10:36:46Z-
dc.date.available2010-04-09T10:36:46Z-
dc.date.issued1994-
dc.identifier.citationO6-alkylguanine-DNA alkyltransferase activity in schistosomiasis-associated human bladder cancer. 1994, 30A (9):1314-9 Eur. J. Canceren
dc.identifier.issn0959-8049-
dc.identifier.pmid7999420-
dc.identifier.doi10.1016/0959-8049(94)90180-5-
dc.identifier.urihttp://hdl.handle.net/10541/96111-
dc.description.abstractO6-Alkylguanine-DNA-alkyltransferase (ATase) activity was measured in extracts of 55 bladder tissue samples (46 tumour and nine uninvolved mucosal tissue) from Egyptian patients with schistosome-associated bladder carcinoma. Activity varied from 2.0 to 16.2 fmole ATase/microgram DNA (mean +/- S.D.; 5.6 +/- 4.0) or from 28 to 351 fmole ATase/mg (117 +/- 71). ATase levels in schistosome-associated bladder cancer tissues (5.6 +/- 4.0 fmole ATase/microgram DNA) tended to be lower than those observed in normal human bladder mucosal tissue (8.5 +/- 4.4 fmole ATase/microgram DNA). In a previous study (Badawi et al., Carcinogenesis, 1992, 13, 877-881) DNA-alkylation damage (O6-methyldeoxyguanosine) was found in 44/46 of these schistosome-associated bladder cancer samples at levels ranging from 0.012 to 0.485 mumole O6-MedG/mole deoxyguanosine. We now report an inverse correlation between the levels of methylation damage and ATase activity (r = -0.67; P < 0.001). These observations encourage further investigations of the possible role of environmental alkylating agents in the aetiology of early bladder cancer associated with schistosomiasis.en
dc.language.isoenen
dc.subjectCancer DNAen
dc.subjectCancer Proteinsen
dc.subjectUrinary Bladder Canceren
dc.subject.meshDNA, Neoplasm-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMethyltransferases-
dc.subject.meshNeoplasm Proteins-
dc.subject.meshO(6)-Methylguanine-DNA Methyltransferase-
dc.subject.meshSchistosomiasis haematobia-
dc.subject.meshUrinary Bladder-
dc.subject.meshUrinary Bladder Neoplasms-
dc.titleO6-alkylguanine-DNA alkyltransferase activity in schistosomiasis-associated human bladder cancer.en
dc.typeArticleen
dc.contributor.departmentNational Center for Toxicology, Jefferson, Arkansas.en
dc.identifier.journalEuropean Journal of Canceren
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