Expression of lineage restricted transcription factors precedes lineage specific differentiation in a multipotent haemopoietic progenitor cell line.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96098
Title:
Expression of lineage restricted transcription factors precedes lineage specific differentiation in a multipotent haemopoietic progenitor cell line.
Authors:
Cross, Michael A; Heyworth, Clare M; Murrell, A M; Bockamp, E O; Dexter, T Michael; Green, Anthony R
Abstract:
Lineage commitment and differentiation are likely to be coordinated by the combined effects of multiple transcription factors acting on numerous different target genes. The mechanisms by which lineage-restricted patterns of transcription factor expression are established are therefore of particular relevance to our understanding of the role of transcription factors both in normal development and in oncogenesis. Here, we report that the genes for the lineage-restricted transcription factors SCL, GATA-1 and GATA-2 are expressed in all multipotent, IL-3-dependent, haemopoietic progenitor cell lines tested. Moreover, a liquid differentiation assay has been used to demonstrate down regulation of SCL, GATA-1, GATA-2 and PU-1 during differentiation into non-expressing lineages. These data support the concept that multiple lineage-restricted transcription factors are expressed prior to lineage commitment.
Affiliation:
CRC Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
Expression of lineage restricted transcription factors precedes lineage specific differentiation in a multipotent haemopoietic progenitor cell line. 1994, 9 (10):3013-6 Oncogene
Journal:
Oncogene
Issue Date:
Oct-1994
URI:
http://hdl.handle.net/10541/96098
PubMed ID:
8084606
Type:
Article
Language:
en
ISSN:
0950-9232
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorCross, Michael Aen
dc.contributor.authorHeyworth, Clare Men
dc.contributor.authorMurrell, A Men
dc.contributor.authorBockamp, E Oen
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorGreen, Anthony Ren
dc.date.accessioned2010-04-09T08:45:06Z-
dc.date.available2010-04-09T08:45:06Z-
dc.date.issued1994-10-
dc.identifier.citationExpression of lineage restricted transcription factors precedes lineage specific differentiation in a multipotent haemopoietic progenitor cell line. 1994, 9 (10):3013-6 Oncogeneen
dc.identifier.issn0950-9232-
dc.identifier.pmid8084606-
dc.identifier.urihttp://hdl.handle.net/10541/96098-
dc.description.abstractLineage commitment and differentiation are likely to be coordinated by the combined effects of multiple transcription factors acting on numerous different target genes. The mechanisms by which lineage-restricted patterns of transcription factor expression are established are therefore of particular relevance to our understanding of the role of transcription factors both in normal development and in oncogenesis. Here, we report that the genes for the lineage-restricted transcription factors SCL, GATA-1 and GATA-2 are expressed in all multipotent, IL-3-dependent, haemopoietic progenitor cell lines tested. Moreover, a liquid differentiation assay has been used to demonstrate down regulation of SCL, GATA-1, GATA-2 and PU-1 during differentiation into non-expressing lineages. These data support the concept that multiple lineage-restricted transcription factors are expressed prior to lineage commitment.en
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshCell Line-
dc.subject.meshCells, Cultured-
dc.subject.meshHematopoiesis-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshRNA, Messenger-
dc.subject.meshTranscription Factors-
dc.titleExpression of lineage restricted transcription factors precedes lineage specific differentiation in a multipotent haemopoietic progenitor cell line.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalOncogeneen

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