Slowly cycling (label-retaining) epidermal cells behave like clonogenic stem cells in vitro.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96085
Title:
Slowly cycling (label-retaining) epidermal cells behave like clonogenic stem cells in vitro.
Authors:
Morris, R J; Potten, Christopher S
Abstract:
Slowly cycling label-retaining epidermal cells were identified by light microscopic autoradiography in the dorsal epidermis and hair follicles of adult mice 8-10 weeks after twice daily injection of [3H]dT on days three through five after birth. Pulse-labelled epidermal cells were identified in the epidermis and hair follicles of 7-8 week old mice 1 h after a single injection of [3H]dT at 8.00 a.m. For mice of both groups, epidermal cells including those from the hair follicles were harvested by trypsinization and were cultured from low density on feeder layers of irradiated Swiss mouse 3T3. On days 2, 4, 5, 7, 10 and 12, the cultures were fixed and processed for light microscopic autoradiography, and the distribution of labelled nuclei was quantified. On day 2 of culture, both label-retaining cells (LRC) and pulse labelled cells (PLC) were found primarily as single cells. After five days, LRC were found as pairs and clusters having silver grain counts consistent with their division. In contrast, PLC remained primarily as single cells. These results suggest that LRC may divide to form colonies (are clonogenic) whereas PLC are rarely clonogenic. The significance of this experiment is that it suggests that the LRC may not only be persistent in the epidermis, but that they may also be cells with relatively greater proliferative potential than the PLC and are thus likely to be stem cells.
Affiliation:
The University of Texas M.D. Anderson Cancer Center, Smithville, USA.
Citation:
Slowly cycling (label-retaining) epidermal cells behave like clonogenic stem cells in vitro. 1994, 27 (5):279-89 Cell Prolif.
Journal:
Cell Proliferation
Issue Date:
May-1994
URI:
http://hdl.handle.net/10541/96085
DOI:
10.1111/j.1365-2184.1994.tb01425.x
PubMed ID:
10465012
Type:
Article
Language:
en
ISSN:
0960-7722
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMorris, R Jen
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2010-04-09T09:21:17Z-
dc.date.available2010-04-09T09:21:17Z-
dc.date.issued1994-05-
dc.identifier.citationSlowly cycling (label-retaining) epidermal cells behave like clonogenic stem cells in vitro. 1994, 27 (5):279-89 Cell Prolif.en
dc.identifier.issn0960-7722-
dc.identifier.pmid10465012-
dc.identifier.doi10.1111/j.1365-2184.1994.tb01425.x-
dc.identifier.urihttp://hdl.handle.net/10541/96085-
dc.description.abstractSlowly cycling label-retaining epidermal cells were identified by light microscopic autoradiography in the dorsal epidermis and hair follicles of adult mice 8-10 weeks after twice daily injection of [3H]dT on days three through five after birth. Pulse-labelled epidermal cells were identified in the epidermis and hair follicles of 7-8 week old mice 1 h after a single injection of [3H]dT at 8.00 a.m. For mice of both groups, epidermal cells including those from the hair follicles were harvested by trypsinization and were cultured from low density on feeder layers of irradiated Swiss mouse 3T3. On days 2, 4, 5, 7, 10 and 12, the cultures were fixed and processed for light microscopic autoradiography, and the distribution of labelled nuclei was quantified. On day 2 of culture, both label-retaining cells (LRC) and pulse labelled cells (PLC) were found primarily as single cells. After five days, LRC were found as pairs and clusters having silver grain counts consistent with their division. In contrast, PLC remained primarily as single cells. These results suggest that LRC may divide to form colonies (are clonogenic) whereas PLC are rarely clonogenic. The significance of this experiment is that it suggests that the LRC may not only be persistent in the epidermis, but that they may also be cells with relatively greater proliferative potential than the PLC and are thus likely to be stem cells.en
dc.language.isoenen
dc.subject.meshAging-
dc.subject.meshAnimals-
dc.subject.meshAnimals, Newborn-
dc.subject.meshAutoradiography-
dc.subject.meshCell Cycle-
dc.subject.meshCell Division-
dc.subject.meshCells, Cultured-
dc.subject.meshClone Cells-
dc.subject.meshEpidermis-
dc.subject.meshFemale-
dc.subject.meshHair Follicle-
dc.subject.meshKeratinocytes-
dc.subject.meshMice-
dc.subject.meshMice, Inbred Strains-
dc.subject.meshStem Cells-
dc.subject.meshTime Factors-
dc.titleSlowly cycling (label-retaining) epidermal cells behave like clonogenic stem cells in vitro.en
dc.typeArticleen
dc.contributor.departmentThe University of Texas M.D. Anderson Cancer Center, Smithville, USA.en
dc.identifier.journalCell Proliferationen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.