Fluorescent location of cells of cytological interest in cervical smears prestained with thionin.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96026
Title:
Fluorescent location of cells of cytological interest in cervical smears prestained with thionin.
Authors:
Steven, F S; Desai, Mina; Sin, J; Palcic, B
Abstract:
Cervical cells of cytological interest were located in conventional smears, treated with thionin for quantitative DNA staining by subsequent treatment with fluorescent probes for a cell surface protease. Normal mature cervical epithelial cells failed to bind these fluorescent probes whilst metaplastic cells, glandular cells, and dyskaryotic cells were readily located. By this means, the nuclear staining of these fluorescent cells of cyotological interest enabled them to be classified by a cytologist.
Affiliation:
Division of Biochemistry, School of Biological Sciences, University of Manchester, U.K.
Citation:
Fluorescent location of cells of cytological interest in cervical smears prestained with thionin., 16 (3A):1193-6 Anticancer Res.
Journal:
Anticancer Research
Issue Date:
8-Apr-2010
URI:
http://hdl.handle.net/10541/96026
PubMed ID:
8702235
Type:
Article
Language:
en
ISSN:
0250-7005
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorSteven, F Sen
dc.contributor.authorDesai, Minaen
dc.contributor.authorSin, Jen
dc.contributor.authorPalcic, Ben
dc.date.accessioned2010-04-08T16:04:28Z-
dc.date.available2010-04-08T16:04:28Z-
dc.date.issued2010-04-08T16:04:28Z-
dc.identifier.citationFluorescent location of cells of cytological interest in cervical smears prestained with thionin., 16 (3A):1193-6 Anticancer Res.en
dc.identifier.issn0250-7005-
dc.identifier.pmid8702235-
dc.identifier.urihttp://hdl.handle.net/10541/96026-
dc.description.abstractCervical cells of cytological interest were located in conventional smears, treated with thionin for quantitative DNA staining by subsequent treatment with fluorescent probes for a cell surface protease. Normal mature cervical epithelial cells failed to bind these fluorescent probes whilst metaplastic cells, glandular cells, and dyskaryotic cells were readily located. By this means, the nuclear staining of these fluorescent cells of cyotological interest enabled them to be classified by a cytologist.en
dc.language.isoenen
dc.subject.meshCarboxylic Ester Hydrolases-
dc.subject.meshCell Nucleus-
dc.subject.meshCervix Uteri-
dc.subject.meshDNA-
dc.subject.meshEndopeptidases-
dc.subject.meshFemale-
dc.subject.meshFluorescent Dyes-
dc.subject.meshHumans-
dc.subject.meshMicroscopy, Fluorescence-
dc.subject.meshPhenothiazines-
dc.subject.meshStaining and Labeling-
dc.titleFluorescent location of cells of cytological interest in cervical smears prestained with thionin.en
dc.typeArticleen
dc.contributor.departmentDivision of Biochemistry, School of Biological Sciences, University of Manchester, U.K.en
dc.identifier.journalAnticancer Researchen
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