Association of thrombospondin-1 with osteogenic differentiation of retinal pericytes in vitro.

2.50
Hdl Handle:
http://hdl.handle.net/10541/96007
Title:
Association of thrombospondin-1 with osteogenic differentiation of retinal pericytes in vitro.
Authors:
Canfield, A E; Sutton, Andrew B; Hoyland, J A; Schor, Ana M
Abstract:
Vascular pericytes can differentiate into osteoblast-like cells in vitro, suggesting that these cells may represent a potential source of osteoprogenitor cells in the adult. Pericyte differentiation is associated with a characteristic pattern of nodule formation and mineralisation. Nodules are formed in post-confluent cultures by the retraction of multilayered areas. Crystals of hydroxyapatite are deposited on the extracellular matrix of these nodules which then becomes mineralised. We now demonstrate that thrombospondin-1 (TSP-1) gene expression is modulated during pericyte differentiation in vitro. That is, the relative levels of TSP-1 (protein and mRNA) increased markedly during nodule formation and then decreased when mineralisation of the nodules had taken place. TSP-1 was localised throughout non-mineralised nodules but it was largely excluded from the inner mass of mineralised nodules. The production of a mineralised matrix by vascular pericytes was promoted by the presence of antibodies to TSP-1 in the culture medium and was inhibited by exogenous TSP-1. These effects did not appear to be mediated through the activation of latent TGF-beta, since neither exogenous TGF-beta nor neutralising antibodies to TGF-beta had any effect on the rate or extent of mineralisation seen in the pericyte cultures. Taken together these results suggest that high levels of TSP-1 inhibit pericyte mineralisation, supporting the view that this protein plays a role in pericyte differentiation and bone formation.
Affiliation:
University of Manchester, School of Biological Sciences, UK.
Citation:
Association of thrombospondin-1 with osteogenic differentiation of retinal pericytes in vitro. 1996, 109 ( Pt 2):343-53 J. Cell. Sci.
Journal:
Journal of Cell Science
Issue Date:
Feb-1996
URI:
http://hdl.handle.net/10541/96007
PubMed ID:
8838658
Type:
Article
Language:
en
ISSN:
0021-9533
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorCanfield, A Een
dc.contributor.authorSutton, Andrew Ben
dc.contributor.authorHoyland, J Aen
dc.contributor.authorSchor, Ana Men
dc.date.accessioned2010-04-08T14:16:38Z-
dc.date.available2010-04-08T14:16:38Z-
dc.date.issued1996-02-
dc.identifier.citationAssociation of thrombospondin-1 with osteogenic differentiation of retinal pericytes in vitro. 1996, 109 ( Pt 2):343-53 J. Cell. Sci.en
dc.identifier.issn0021-9533-
dc.identifier.pmid8838658-
dc.identifier.urihttp://hdl.handle.net/10541/96007-
dc.description.abstractVascular pericytes can differentiate into osteoblast-like cells in vitro, suggesting that these cells may represent a potential source of osteoprogenitor cells in the adult. Pericyte differentiation is associated with a characteristic pattern of nodule formation and mineralisation. Nodules are formed in post-confluent cultures by the retraction of multilayered areas. Crystals of hydroxyapatite are deposited on the extracellular matrix of these nodules which then becomes mineralised. We now demonstrate that thrombospondin-1 (TSP-1) gene expression is modulated during pericyte differentiation in vitro. That is, the relative levels of TSP-1 (protein and mRNA) increased markedly during nodule formation and then decreased when mineralisation of the nodules had taken place. TSP-1 was localised throughout non-mineralised nodules but it was largely excluded from the inner mass of mineralised nodules. The production of a mineralised matrix by vascular pericytes was promoted by the presence of antibodies to TSP-1 in the culture medium and was inhibited by exogenous TSP-1. These effects did not appear to be mediated through the activation of latent TGF-beta, since neither exogenous TGF-beta nor neutralising antibodies to TGF-beta had any effect on the rate or extent of mineralisation seen in the pericyte cultures. Taken together these results suggest that high levels of TSP-1 inhibit pericyte mineralisation, supporting the view that this protein plays a role in pericyte differentiation and bone formation.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCattle-
dc.subject.meshCell Differentiation-
dc.subject.meshCells, Cultured-
dc.subject.meshDNA-
dc.subject.meshFemale-
dc.subject.meshMembrane Glycoproteins-
dc.subject.meshOsteogenesis-
dc.subject.meshProtein Biosynthesis-
dc.subject.meshRetina-
dc.subject.meshThrombospondins-
dc.subject.meshTransforming Growth Factor beta-
dc.titleAssociation of thrombospondin-1 with osteogenic differentiation of retinal pericytes in vitro.en
dc.typeArticleen
dc.contributor.departmentUniversity of Manchester, School of Biological Sciences, UK.en
dc.identifier.journalJournal of Cell Scienceen

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