Distinct mechanisms for rescue from apoptosis in Ramos human B cells by signaling through CD40 and interleukin-4 receptor: role for inhibition of an early response gene, Berg36.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95934
Title:
Distinct mechanisms for rescue from apoptosis in Ramos human B cells by signaling through CD40 and interleukin-4 receptor: role for inhibition of an early response gene, Berg36.
Authors:
Ning, Z Q; Norton, John D; Li, J; Murphy, J J
Abstract:
The role of interleukin-4 (IL-4) and CD40 signaling in negative regulation of apoptosis in human Ramos B cells induced in response to different agents was investigated. CD40 ligation protected cells from apoptosis induced by calcium ionophore through an initial, rapid and apparently Bcl-2-independent mechanism, associated with up-regulation of Bcl-XL. However, rescue from apoptosis induced by inhibition of macromolecular synthesis required several hours of prior stimulation with CD40 ligand/antibody and was accompanied by up-regulation of Bcl-2. In contrast, IL-4 did not up-regulate Bcl-2 or Bcl-XL and did not inhibit apoptosis induced by inhibitors of macromolecular synthesis. However, IL-4 did protect Ramos cells from apoptosis induced by calcium ionophore and this effect was accompanied by inhibition of ionophore-induced expression of an immediate early gene encoding a 36-kDa zinc-finger protein, Berg36. Antisense blockade of Berg36 expression partially inhibited ionophore-induced apoptosis to an extent commensurate with the level of IL-4 protection, implicating Berg36 function as a requirement for apoptosis induced through calcium signaling and as a target for IL-4 through which this cytokine inhibits apoptosis in Ramos B cells. These distinct mechanisms for rescue from apoptosis by CD40 and IL-4 may help explain the co-operative roles of these T cell-derived signals for B cell survival.
Affiliation:
Infection and Immunity Research group, King's College London, GB.
Citation:
Distinct mechanisms for rescue from apoptosis in Ramos human B cells by signaling through CD40 and interleukin-4 receptor: role for inhibition of an early response gene, Berg36. 1996, 26 (10):2356-63 Eur. J. Immunol.
Journal:
European Journal of Immunology
Issue Date:
Oct-1996
URI:
http://hdl.handle.net/10541/95934
DOI:
10.1002/eji.1830261013
PubMed ID:
8898945
Type:
Article
Language:
en
ISSN:
0014-2980
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorNing, Z Qen
dc.contributor.authorNorton, John Den
dc.contributor.authorLi, Jen
dc.contributor.authorMurphy, J Jen
dc.date.accessioned2010-04-07T15:33:19Z-
dc.date.available2010-04-07T15:33:19Z-
dc.date.issued1996-10-
dc.identifier.citationDistinct mechanisms for rescue from apoptosis in Ramos human B cells by signaling through CD40 and interleukin-4 receptor: role for inhibition of an early response gene, Berg36. 1996, 26 (10):2356-63 Eur. J. Immunol.en
dc.identifier.issn0014-2980-
dc.identifier.pmid8898945-
dc.identifier.doi10.1002/eji.1830261013-
dc.identifier.urihttp://hdl.handle.net/10541/95934-
dc.description.abstractThe role of interleukin-4 (IL-4) and CD40 signaling in negative regulation of apoptosis in human Ramos B cells induced in response to different agents was investigated. CD40 ligation protected cells from apoptosis induced by calcium ionophore through an initial, rapid and apparently Bcl-2-independent mechanism, associated with up-regulation of Bcl-XL. However, rescue from apoptosis induced by inhibition of macromolecular synthesis required several hours of prior stimulation with CD40 ligand/antibody and was accompanied by up-regulation of Bcl-2. In contrast, IL-4 did not up-regulate Bcl-2 or Bcl-XL and did not inhibit apoptosis induced by inhibitors of macromolecular synthesis. However, IL-4 did protect Ramos cells from apoptosis induced by calcium ionophore and this effect was accompanied by inhibition of ionophore-induced expression of an immediate early gene encoding a 36-kDa zinc-finger protein, Berg36. Antisense blockade of Berg36 expression partially inhibited ionophore-induced apoptosis to an extent commensurate with the level of IL-4 protection, implicating Berg36 function as a requirement for apoptosis induced through calcium signaling and as a target for IL-4 through which this cytokine inhibits apoptosis in Ramos B cells. These distinct mechanisms for rescue from apoptosis by CD40 and IL-4 may help explain the co-operative roles of these T cell-derived signals for B cell survival.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAntigens, CD-
dc.subject.meshAntigens, CD40-
dc.subject.meshApoptosis-
dc.subject.meshB-Lymphocytes-
dc.subject.meshBase Sequence-
dc.subject.meshBurkitt Lymphoma-
dc.subject.meshButyrate Response Factor 1-
dc.subject.meshCalcium-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshGene Expression-
dc.subject.meshGenes, Immediate-Early-
dc.subject.meshHumans-
dc.subject.meshImmediate-Early Proteins-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshProto-Oncogene Proteins c-bcl-2-
dc.subject.meshRNA, Messenger-
dc.subject.meshReceptors, Interleukin-
dc.subject.meshReceptors, Interleukin-4-
dc.subject.meshSignal Transduction-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshZinc Fingers-
dc.titleDistinct mechanisms for rescue from apoptosis in Ramos human B cells by signaling through CD40 and interleukin-4 receptor: role for inhibition of an early response gene, Berg36.en
dc.typeArticleen
dc.contributor.departmentInfection and Immunity Research group, King's College London, GB.en
dc.identifier.journalEuropean Journal of Immunologyen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.