Influence of cell interactions in a novel model of postnatal mucosal regeneration.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95886
Title:
Influence of cell interactions in a novel model of postnatal mucosal regeneration.
Authors:
Patel, H R; Tait, I S; Evans, Gareth S; Campbell, F C
Abstract:
BACKGROUND AND AIMS: Conventional models of postnatal mucosal regeneration are cumbersome and limited: a novel model is described here. In addition, the influence of cell interactions on mucosal regeneration is examined within the model. METHODS: Postnatal rat small intestinal mucosa was digested by enzymes to yield heterotypic cell aggregates (CA). CA colony forming ability, growth, and limited cytodifferentiation were assessed in vitro. CA were transplanted subcutaneously and retrieved for histological examination at staggered intervals to assess neomucosal morphogenesis and cytodifferentiation in vivo. Cell interactions in CA were disrupted by enzymes, thus producing cell suspensions (CS). Regeneration by CA and CS were compared. RESULTS: CA produced proliferative colonies in vitro and showed a temporal sequence of neomucosal morphogenesis and differentiation in vivo. CA colonies were more numerous within 24 hours of primary culture and had greater cellularity by 96 hours than CS colonies. Alkaline phosphatase was expressed only by 258 of 696 CA colonies (37%). CA subcutaneous grafts (48 of 56 (87%)) regenerated small intestinal neomucosa while CS were unsuccessful. CONCLUSION: These methods provide a model of mucosal regeneration which includes constituent processes of colony formation, growth, neomucosal morphogenesis, and cytodifferentiation. Preservation of cell interactions within CA seems advantageous to regeneration within the model.
Affiliation:
University Department of Surgery, Ninewells Hospital and Medical School, Dundee.
Citation:
Influence of cell interactions in a novel model of postnatal mucosal regeneration. 1996, 38 (5):679-86 Gut
Journal:
Gut
Issue Date:
May-1996
URI:
http://hdl.handle.net/10541/95886
DOI:
10.1136/gut.38.5.679
PubMed ID:
8707112
Type:
Article
Language:
en
ISSN:
0017-5749
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPatel, H Ren
dc.contributor.authorTait, I Sen
dc.contributor.authorEvans, Gareth Sen
dc.contributor.authorCampbell, F Cen
dc.date.accessioned2010-04-07T13:58:03Z-
dc.date.available2010-04-07T13:58:03Z-
dc.date.issued1996-05-
dc.identifier.citationInfluence of cell interactions in a novel model of postnatal mucosal regeneration. 1996, 38 (5):679-86 Guten
dc.identifier.issn0017-5749-
dc.identifier.pmid8707112-
dc.identifier.doi10.1136/gut.38.5.679-
dc.identifier.urihttp://hdl.handle.net/10541/95886-
dc.description.abstractBACKGROUND AND AIMS: Conventional models of postnatal mucosal regeneration are cumbersome and limited: a novel model is described here. In addition, the influence of cell interactions on mucosal regeneration is examined within the model. METHODS: Postnatal rat small intestinal mucosa was digested by enzymes to yield heterotypic cell aggregates (CA). CA colony forming ability, growth, and limited cytodifferentiation were assessed in vitro. CA were transplanted subcutaneously and retrieved for histological examination at staggered intervals to assess neomucosal morphogenesis and cytodifferentiation in vivo. Cell interactions in CA were disrupted by enzymes, thus producing cell suspensions (CS). Regeneration by CA and CS were compared. RESULTS: CA produced proliferative colonies in vitro and showed a temporal sequence of neomucosal morphogenesis and differentiation in vivo. CA colonies were more numerous within 24 hours of primary culture and had greater cellularity by 96 hours than CS colonies. Alkaline phosphatase was expressed only by 258 of 696 CA colonies (37%). CA subcutaneous grafts (48 of 56 (87%)) regenerated small intestinal neomucosa while CS were unsuccessful. CONCLUSION: These methods provide a model of mucosal regeneration which includes constituent processes of colony formation, growth, neomucosal morphogenesis, and cytodifferentiation. Preservation of cell interactions within CA seems advantageous to regeneration within the model.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Communication-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Division-
dc.subject.meshCells, Cultured-
dc.subject.meshIntestinal Mucosa-
dc.subject.meshIntestine, Small-
dc.subject.meshRats-
dc.subject.meshRats, Inbred Strains-
dc.subject.meshRegeneration-
dc.subject.meshTissue Transplantation-
dc.titleInfluence of cell interactions in a novel model of postnatal mucosal regeneration.en
dc.typeArticleen
dc.contributor.departmentUniversity Department of Surgery, Ninewells Hospital and Medical School, Dundee.en
dc.identifier.journalGuten

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