Murine gammaherpesvirus-68 encodes homologues of thymidine kinase and glycoprotein H: sequence, expression, and characterization of pyrimidine kinase activity.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95846
Title:
Murine gammaherpesvirus-68 encodes homologues of thymidine kinase and glycoprotein H: sequence, expression, and characterization of pyrimidine kinase activity.
Authors:
Pepper, Stuart D; Stewart, J Philip; Arrand, John R; Mackett, Mike
Abstract:
We have sequenced a 4.5-kb fragment of DNA spanning the junction of the BamHI D and E fragments of murine gammaherpesvirus-68 (MHV-68). This sequence was found to code for two major open reading frames (orfs) of 1934 and 2192 bp which showed significant homology to the thymidine kinase (TK) and glycoprotein H (gH) sequences of other gammaherpesviruses. Upstream from the TK gene another orf was found which showed amino acid sequence homology to the HSV1 UL24 gene. Analysis of the 1934-bp orf revealed the presence of all six of the recognized sites that are conserved between herpesvirus TKs although, uniquely among sequenced herpesvirus TK enzymes, MHV-68 lacks the consensus nucleotide binding site GXXGXGK, the second glycine being replaced by alanine. The MHV-68 TK has a predicted M(r) of 68,443, while the gH is predicted to have a M(r) of 82,890. Northern blot analysis showed an early TK message of 2.6 kb and a late gH-specific message of 2.5 kb. Both TK and gH probes detected a 4.3-kb late message, implying that this late message spans gH and TK. The TK coding sequence was expressed using an in vitro transcription translation system and was shown to encode functional TK activity.
Affiliation:
CRC Department of Molecular Biology, Paterson Institute for Cancer Research, Christie CRC Research Centre, Christie Hospit, Withington, Manchester, United Kingdom.
Citation:
Murine gammaherpesvirus-68 encodes homologues of thymidine kinase and glycoprotein H: sequence, expression, and characterization of pyrimidine kinase activity. 1996, 219 (2):475-9 Virology
Journal:
Virology
Issue Date:
15-May-1996
URI:
http://hdl.handle.net/10541/95846
DOI:
10.1006/viro.1996.0274
PubMed ID:
8638414
Type:
Article
Language:
en
ISSN:
0042-6822
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPepper, Stuart Den
dc.contributor.authorStewart, J Philipen
dc.contributor.authorArrand, John Ren
dc.contributor.authorMackett, Mikeen
dc.date.accessioned2010-04-07T10:41:04Z-
dc.date.available2010-04-07T10:41:04Z-
dc.date.issued1996-05-15-
dc.identifier.citationMurine gammaherpesvirus-68 encodes homologues of thymidine kinase and glycoprotein H: sequence, expression, and characterization of pyrimidine kinase activity. 1996, 219 (2):475-9 Virologyen
dc.identifier.issn0042-6822-
dc.identifier.pmid8638414-
dc.identifier.doi10.1006/viro.1996.0274-
dc.identifier.urihttp://hdl.handle.net/10541/95846-
dc.description.abstractWe have sequenced a 4.5-kb fragment of DNA spanning the junction of the BamHI D and E fragments of murine gammaherpesvirus-68 (MHV-68). This sequence was found to code for two major open reading frames (orfs) of 1934 and 2192 bp which showed significant homology to the thymidine kinase (TK) and glycoprotein H (gH) sequences of other gammaherpesviruses. Upstream from the TK gene another orf was found which showed amino acid sequence homology to the HSV1 UL24 gene. Analysis of the 1934-bp orf revealed the presence of all six of the recognized sites that are conserved between herpesvirus TKs although, uniquely among sequenced herpesvirus TK enzymes, MHV-68 lacks the consensus nucleotide binding site GXXGXGK, the second glycine being replaced by alanine. The MHV-68 TK has a predicted M(r) of 68,443, while the gH is predicted to have a M(r) of 82,890. Northern blot analysis showed an early TK message of 2.6 kb and a late gH-specific message of 2.5 kb. Both TK and gH probes detected a 4.3-kb late message, implying that this late message spans gH and TK. The TK coding sequence was expressed using an in vitro transcription translation system and was shown to encode functional TK activity.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshGammaherpesvirinae-
dc.subject.meshGene Expression-
dc.subject.meshGlycoproteins-
dc.subject.meshMice-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshOpen Reading Frames-
dc.subject.meshRNA, Viral-
dc.subject.meshThymidine Kinase-
dc.subject.meshViral Envelope Proteins-
dc.titleMurine gammaherpesvirus-68 encodes homologues of thymidine kinase and glycoprotein H: sequence, expression, and characterization of pyrimidine kinase activity.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Molecular Biology, Paterson Institute for Cancer Research, Christie CRC Research Centre, Christie Hospit, Withington, Manchester, United Kingdom.en
dc.identifier.journalVirologyen

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