Metastasis-associated 5T4 antigen disrupts cell-cell contacts and induces cellular motility in epithelial cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/95843
Title:
Metastasis-associated 5T4 antigen disrupts cell-cell contacts and induces cellular motility in epithelial cells.
Authors:
Carsberg, Catherine J; Myers, Kevin A; Stern, Peter L
Abstract:
The 5T4 antigen is defined by a monoclonal antibody (MAb) specific for human trophoblast. It is also expressed by many types of tumour cell and has been associated with metastasis and poor clinical outcome in a number of cancers. This pattern of expression is consistent with a mechanistic involvement of 5T4 molecules in the spread of cancer cells. The 5T4 antigen is a transmembrane glycoprotein with a 310 amino acid extracellular domain and a 44 amino acid cytoplasmic domain. Transfection of full-length 5T4 cDNA into epithelial cells alters cell-cell contacts and cellular motility. Thus, in 5T4-transfected CL-S1 murine mammary cells, 5T4 expression is associated with dendritic morphology, accompanied by abrogation of actin/cadherin-containing contacts and increased motility. In transfected MDCK canine kidney epithelial cells, 5T4 over-expression also results in increased motility, but disruption of cell-cell contacts, either by culturing cells in low calcium medium or by addition of HGF/SF, is needed. The effects of 5T4 expression on morphology and motility are separable since cells transfected with a truncated form of 5T4 cDNA in which the cytoplasmic domain is deleted reveal that the latter is necessary to abrogate actin/cadherin-containing contacts but does not influence the effects on motility. Thus, 5T4 molecules can deliver signals through both the extracellular and intracellular domains, and the resultant effects are consistent with a role for 5T4 molecules in invasion processes.
Affiliation:
CRC Department of Immunology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
Metastasis-associated 5T4 antigen disrupts cell-cell contacts and induces cellular motility in epithelial cells. 1996, 68 (1):84-92 Int. J. Cancer
Journal:
International Journal of Cancer
Issue Date:
27-Sep-1996
URI:
http://hdl.handle.net/10541/95843
DOI:
10.1002/(SICI)1097-0215(19960927)68:1<84::AID-IJC15>3.0.CO;2-6
PubMed ID:
8895545
Type:
Article
Language:
en
ISSN:
0020-7136
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorCarsberg, Catherine Jen
dc.contributor.authorMyers, Kevin Aen
dc.contributor.authorStern, Peter Len
dc.date.accessioned2010-04-07T10:35:42Z-
dc.date.available2010-04-07T10:35:42Z-
dc.date.issued1996-09-27-
dc.identifier.citationMetastasis-associated 5T4 antigen disrupts cell-cell contacts and induces cellular motility in epithelial cells. 1996, 68 (1):84-92 Int. J. Canceren
dc.identifier.issn0020-7136-
dc.identifier.pmid8895545-
dc.identifier.doi10.1002/(SICI)1097-0215(19960927)68:1<84::AID-IJC15>3.0.CO;2-6-
dc.identifier.urihttp://hdl.handle.net/10541/95843-
dc.description.abstractThe 5T4 antigen is defined by a monoclonal antibody (MAb) specific for human trophoblast. It is also expressed by many types of tumour cell and has been associated with metastasis and poor clinical outcome in a number of cancers. This pattern of expression is consistent with a mechanistic involvement of 5T4 molecules in the spread of cancer cells. The 5T4 antigen is a transmembrane glycoprotein with a 310 amino acid extracellular domain and a 44 amino acid cytoplasmic domain. Transfection of full-length 5T4 cDNA into epithelial cells alters cell-cell contacts and cellular motility. Thus, in 5T4-transfected CL-S1 murine mammary cells, 5T4 expression is associated with dendritic morphology, accompanied by abrogation of actin/cadherin-containing contacts and increased motility. In transfected MDCK canine kidney epithelial cells, 5T4 over-expression also results in increased motility, but disruption of cell-cell contacts, either by culturing cells in low calcium medium or by addition of HGF/SF, is needed. The effects of 5T4 expression on morphology and motility are separable since cells transfected with a truncated form of 5T4 cDNA in which the cytoplasmic domain is deleted reveal that the latter is necessary to abrogate actin/cadherin-containing contacts but does not influence the effects on motility. Thus, 5T4 molecules can deliver signals through both the extracellular and intracellular domains, and the resultant effects are consistent with a role for 5T4 molecules in invasion processes.en
dc.language.isoenen
dc.subjectCancer Antigensen
dc.subjectCancer Metastasisen
dc.subject.meshAnimals-
dc.subject.meshAntigens, Neoplasm-
dc.subject.meshBlotting, Western-
dc.subject.meshCell Communication-
dc.subject.meshCell Line-
dc.subject.meshCell Movement-
dc.subject.meshCytoskeleton-
dc.subject.meshDogs-
dc.subject.meshEpithelium-
dc.subject.meshFlow Cytometry-
dc.subject.meshFluorescent Antibody Technique-
dc.subject.meshHumans-
dc.subject.meshKidney-
dc.subject.meshMembrane Glycoproteins-
dc.subject.meshMice-
dc.subject.meshMice, Inbred BALB C-
dc.subject.meshNeoplasm Metastasis-
dc.subject.meshTransfection-
dc.titleMetastasis-associated 5T4 antigen disrupts cell-cell contacts and induces cellular motility in epithelial cells.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Immunology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalInternational Journal of Canceren

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.